Ðe kansa lãmenugbagbeviwo le wò ʋu mea? Nukae dɔlélenutsiŋutete ƒe dodokpɔa dea dzesii?

Ðe wò ɖɔkta lé ŋku ɖe wò Ʋu Xexlẽme Blibo (CBC) ŋu hegblɔ be wò ʋumenugbagbevi ɣiwo ƒe xexlẽme sɔ gbɔ vie alo edze abe ɖe wòsɔ enea? Eye emegbe eƒo nu tso dodokpɔ aɖe si ŋu ŋkɔ wɔnuku aɖe le si woyɔna be `Immunophenotyping` ŋua? Esi nèse ŋkɔ ma la, ɖewohĩ vɔvɔ̃ ɖo wò vie, henɔ dodokpɔ ka ƒomevie nye esia ŋu bum. Enye nusi bɔ ŋutɔ. Eyata egbea, mina míaƒo nu tso dodokpɔ sia si woyɔna be `Immunophenotyping` ŋu le mɔ bɔbɔe aɖe ŋutɔ nu, le mɔ si gɔme nàte ŋu ase nu.

Nukae nye dɔlélenutsiŋutete ƒe nɔnɔme dzro ko?

Kpuie ko la, `Immunophenotyping` nye dodokpɔ tɔxɛ aɖe si wowɔna le dodokpɔxɔ me si léa ŋku ɖe lãmenugbagbevi siwo le míaƒe ŋutilã me ŋu, vevietɔ ʋumenugbagbevi ɣiwo, be woakpɔe ɖa be wonye kansa alo menyee o. Esia kpena ɖe ame ŋu vevietɔ le kansa siwo do ƒome kple ʋu abe ʋumenugbagbeviɣidɔ kple ʋumenugbagbeviɣidɔ ene didi me.

Le dodokpɔ sia me la, dɔléleŋutinunyala, ɖɔkta si bi ɖe lãkusiwo kple lãmenugbagbeviwo me, dia protein tɔxɛ siwo woyɔna be ``antigens'' le wò ʋumenugbagbevi ɣiwo ƒe anyime. ``Antigens'' siawo ƒe anyinɔnɔ, kple ``antigens'' ƒomevi si le eme, ateŋu akpeɖe ame ŋu wòanya nenye be lãmenugbagbevi si ŋu nya ku ɖo la le lãmesẽ me alo kansa le eŋu.

Ne míelé ŋku ɖe gɔmesese si le nya `Immunophenotyping` ŋu la, míate ŋu aɖe esia me ayi ŋgɔe:

• Dɔlélenutsiŋutete: Esia fia nane si do ƒome kple míaƒe dɔlélenutsiŋutete . Le dodokpɔ sia me la, wozãa protein siwo woyɔna be ``Antigens'' siwo meyɔ va yi tsɔ dea dzesi protein ƒomevi bubu si woyɔna be ``Antibodies'' si hã le míaƒe dɔlélenutsiŋutete me. Esia le abe safui kple gaƒoɖokui ene. Safui aɖe koŋ (Antibody) si wowɔ nɛ koe sɔ na gaƒoɖokui aɖe koŋ (Antigen).

• Phenotype: Esia fia nane ƒe gotagome nɔnɔme siwo woate ŋu akpɔ . Eyata ne wotsɔ safui ma (Antibody) kpe ɖe gaƒoɖokui (Antigen) ŋu la, míate ŋu akpɔe le mɔ aɖe nu. Ne dɔléleŋutinunyala kpɔ ema la, enya be gavi si sɔ, si nye `(Antigen)` si sɔ la, le lãmenugbagbevi ma dzi.

`(Antigens)` (locks) siawo siwo le kansa lãmenugbagbeviwo me la to vovo tso esiwo le lãmenugbagbevi siwo le lãmesẽ me gbɔ. Eyata to dzesidede `(Antigens)` ƒomevi siawo tututu me la, dodokpɔ sia kpena ɖe ame ŋu be woade dzesii kple kakaɖedzi nenye be kansa lãmenugbagbevie nye esia, eye ne ele nenema la, kansa ƒomevi ka ƒomevie wònye.

Ɣekaɣie wòle be woawɔ dodokpɔ sia?

Zi geɖe la, wò ɖɔkta aɖo aɖaŋu na wò dodokpɔ sia ne wò ʋu ƒe xexlẽme bliboa (CBC) ɖee fia be ʋumenugbagbevi ɣiwo ƒe xexlẽme sɔ gbɔ le mɔ si mesɔ o nu. Esia ate ŋu anye ʋumenugbagbeviɣidɔ aɖewo ƒe dzesi. Eyata, ``Immunophenotyping`` dodokpɔa kpena ɖe ame ŋu be woaɖo kpe ɖikeke ma dzi.

Mɔnu sia le vevie ŋutɔ le kansa ŋuti numekuku hã me. Wozãa mɔ̃ɖaŋununya sia hã tsɔ srɔ̃a nu tso alesi kansa lãmenugbagbevi siwo si ``Antigens`` aɖewo le wɔa nu ɖe ​​kansa ƒe atike vovovowo ŋu, vevietɔ ``Immunotherapy`` ƒe atikewɔwɔwo.

Aleke wowɔa dodokpɔae? Nukae dzɔna le dodokpɔxɔa me?

Le dodokpɔ sia me la, nu sue aɖe koe wòle be nàwɔ. Emae nye be woana kpɔɖeŋu si hiã na dodokpɔa. Le ema megbe la, nu bubu ɖesiaɖe dzɔna le dodokpɔxɔa me. Mehiã be nàtsi dzi ɖe eŋu o. Zi geɖe la, wozãa mɔnu vevi eve tsɔ wɔa dodokpɔ sia.

1. Sisi ƒe Lãmetsiŋusẽŋutinunya

2. Dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe dɔwɔwɔ (IHC) .

Na míakpɔ nusi dzɔna le mɔnu eve siawo me ɖa.

Flow Cytometry Mɔnu

Esiae nye mɔnu si wozãna wu. Mɔnu sia wɔnɛ be woate ŋu ado lãmenugbagbevi gbogbo aɖewo siwo woɖe tso ʋu alo lãkusi me kpɔ zi ɖeka. Nusiwo dzɔna le dodokpɔxɔa me enye si:

• Kpɔɖeŋua ɖeɖe: Zi geɖe la, wowɔa esia to wò ƒumeŋuɖui ƒe kpɔɖeŋu sue aɖe ɖeɖe me (ƒumeŋuɖui ƒe lãmenugbagbeviwo ɖeɖe). Alo, zi geɖe la, to ʋu sue aɖe nono me. Ɣeaɖewoɣi la, woate ŋu axɔ aklã me tsi alo ƒodo me tsi hã.
• Kpɔɖeŋua takpɔkpɔ: Le dodokpɔxɔa me la, wodzraa kpɔɖeŋua ɖo to atike tɔxɛwo dede eme be lãmenugbagbeviawo nanɔ anyi eye woagaku o.
• Dɔlékuiwutikewo Tsɔtsɔ De Eme: Emegbe akpa vevitɔ kekeake va. Wotsɔa ``Antibody'' (key) si wowɔ be wòasɔ kple ``Antigen'' si dim míele la kpena ɖe kpɔɖeŋu sia ŋu. Gake nu tɔxɛ aɖe le ``Antibody'' sia ŋu. Nu sue aɖe le esi si doa kekeli, si nye ``Fluorescent Compound'' si wotsɔ kpe ɖe eŋu. Eyata ne safui ma do ka kple gaƒoɖokui la, lãmenugbagbevi ma dzea kekeli dodo gɔme.
• Dede Flow Cytometer me: Emegbe wotsɔa kpɔɖeŋu sia si wodzra ɖo la dea mɔ̃ tɔxɛ aɖe si woyɔna be ``Flow Cytometer.`` Esi lãmenugbagbeviawo le zɔzɔm to mɔ̃ sia me ɖekaɖeka la, sensor ``Detectors`` siwo le mɔ̃a me dea dzesi lãmenugbagbevi siwo doa kekeli si klẽna eye woxlẽa wo.

To mɔnu sia dzi la, woate ŋu anya lãmenugbagbevi agbɔsɔsɔme si me `(Antigen)` si sɔ la le pɛpɛpɛ ŋutɔ.

Dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe dɔwɔwɔ (IHC) mɔnu

Zi geɖe la, wozãa mɔnu sia tsɔ dia `(Antigens)` siwo le lãkusi ƒe kpɔɖeŋu tɔxɛ aɖe me `(Tissue Sample). Le afisia la, nane si sɔ kple `(Flow Cytometry)` dzɔna. Gake vovototo gãtɔ enye be le afisia la, `(Antibody)` la meku ɖe fluorescent compound ŋu o. Ke boŋ wotsɔa enzyme `(Enzyme)` kpena ɖe eŋu.

Le enzyme sia ta la, ne `(Antibody)` la bla ɖe `(Antigen)` si sɔ ŋu la, lãmenugbagbevi ma trɔa amadede aɖe koŋ. Emegbe ne dɔléleŋutinunyala la lé ŋku ɖe eŋu to nusuekpɔmɔ̃ dzi la, ate ŋu akpɔ lãmenugbagbevi mawo siwo le amadede vovovowo me la nyuie. To mɔ sia dzi la, ate ŋu aɖo kpe edzi be `(Antigen)` si sɔ la le lãkusi ma me.

Ðe seɖoƒe alo gbɔdzɔgbɔdzɔ aɖewo le dodokpɔ sia ŋua?

Ẽ, abe dodokpɔwo katã ene la, seɖoƒe li na esiawo. Kuxi gãtɔe nye be menye dodokpɔxɔ ɖesiaɖe si wɔa dodokpɔ siae zɔna ɖe mɔnu ɖeka ma ke si wozãna ɖaa dzi o. Esia fia be vovototo sue aɖe ate ŋu anɔ emetsonuwo me tso dodokpɔxɔ ɖeka me yi bubu me.

Nu ka ƒomevie dona le nyatakakaa me? Nya kae wògblɔ?

`Immunophenotyping` dodokpɔ ƒe nyatakaka mele bɔbɔe abe ʋu ƒe nyatakaka si wogblɔna edziedzi ene o. Nyatakaka kple nɔnɔmetata siwo woxɔ tso `(Flow Cytometer)` mɔ̃a me le eme. Dɔléleŋutinunyala si xɔ hehe koe ate ŋu ase nusiawo gɔme. Edzroa nyatakaka mawo me eye wòdzraa nyatakaka mamlɛtɔ ɖo.

Tso nyatakaka ma me la, wò ɖɔkta axɔ nyatakaka siawo tɔgbe:

• Ʋumenugbagbevi ɣi ka ƒomevie le kpɔɖeŋua me?
• Tsitsi ƒe akpa ka mee lãmenugbagbevi mawo le?
• Lãmenugbagbevi nenie me `(Antigen)` si wodo kpɔ la le?

Dɔléleŋutinunyala la tsɔa nyatakaka siwo tso wò kpɔɖeŋua me sɔna kple nyatakaka siwo tso ame aɖe si le lãmesẽ me gbɔ, kpakple nyatakaka siwo tso amesiwo ŋu ʋumenugbagbeviɣidɔ alo ʋumenugbagbeviɣidɔ ƒomevi aɖe koŋ le gbɔ. Enɔa te ɖe nusiwo wotsɔ sɔ kple wo nɔewo sia dzi, tsɔ kpe ɖe dodokpɔ bubuwo me tsonu ŋu, ƒo nya ta tso alesi wòanya wɔ be kansa si wòsusu be yeaxɔ wò ŋu.

Ɣekaɣie wòle be maƒo nu kple ɖɔktaa?

Dodokpɔ me tsonu lala ate ŋu anye nuteɖeamedzi. Nyabiase akpe ɖeka le susu me na wò. Enye nusi sɔ be nàse le ɖokuiwò me be kakaɖedzi mele ɣeyiɣi si me emetsonuwo ava kple nya si woagblɔ ŋu o.

Ðewohĩ kuxi aɖeke mele ŋuwò o, ɖewohĩ ewɔ nuku na wò be nèkpɔ `CBC` ƒe nyatakakaa. Ðewohĩ ɖeɖi te ŋuwò vie eye yeƒe lãme mekɔ o hena ɣeyiɣi aɖe. Ke hã, ɣeyiɣi sia nye esi wòle be míagbɔ dzi ɖi. Esia nye afɔɖeɖe ɖeka ko le mɔzɔzɔa me be nànya nusi le edzi yim le wò ŋutilã me.

Wò ɖɔktae nye wò mɔfiala nyuitɔ kekeake le ɣeyiɣi sia me.

• Ne mèka ɖe ɣeyiɣi si me nàkpɔ emetsonuwo dzi o la, bia wò ɖɔkta.
• Ne èxɔ wò numekukua me tsonu vɔ la, ne mènya nusi le wo me nyuie o la, bia nyawo vaseɖe esime nàse egɔme. Emae nye wò gomenɔamesi.
• Mègatɔtɔ le nusiwo le nyatakakaa me ta o. Enye ɖɔkta ƒe agbanɔamedzi be wòaɖe nusi wòfia na wò la me.

Nyatakaka geɖe le wò lãmenugbagbeviwo me ku ɖe wò lãmesẽ ŋu. Dɔlékuiwutikewo ƒe dɔwɔwɔ nye dɔwɔnu ɖeka ko si ate ŋu akpe ɖe ŋuwò nàke ɖe nyatakaka mawo ŋu eye nàse wò lãmesẽ gɔme nyuie wu. Enye nusi sɔ be vɔvɔ̃ kple dzimaɖitsitsi le ɣeyiɣi sia tɔgbe me. Ne èhiã kpekpeɖeŋu la, ƒo nu tso eŋu na wò ɖɔkta.