Yu tink se kansa sɛl dɛn de na yu blɔd? Wetin di immunophenotyping test detekt?

Yu dɔktɔ luk yu Kɔmplit Blɔd Kɔnt (CBC) ɛn se yu wayt blɔd sɛl kɔnt bɔku smɔl ɔ i luk abnɔmal? Ɛn afta dat i tɔk bɔt wan tɛst wit wan strenj nem we dɛn kɔl `Immunophenotyping`? We yu yɛri da nem de, sɔntɛm yu bin de fred smɔl, ɛn yu bin de wɔnda us kayn tɛst dis. Na tin we rili kɔmɔn. So tide, lɛ wi tɔk bɔt dis tɛst we dɛn kɔl `Immunophenotyping` insay wan rili simpul we, insay wan we we yu go ɔndastand.

Wetin na immunophenotyping simpul wan?

Fɔ tɔk am simpul wan, `Immunophenotyping` na spɛshal lab tɛst we de luk di sɛl dɛn na wi bɔdi, mɔ di wayt blɔd sɛl dɛn, fɔ si if dɛn gɛt kansa ɔ nɔ gɛt kansa. Dis kin ɛp mɔ fɔ no di kansa dɛn we gɛt fɔ du wit blɔd lɛk lukimiya ɛn limfoma.

insay dis tεst, wan patכlayz, we na dכkta we spεshal pan tisu dεm εn sεl dεm, de luk fכ spεsifi k protin dεm we dεn kכl ``antijen'' na di sεf fכ yu wayt bכdi sεl dεm. di prεsεns fכ dεn ``antijen'' dεm, εn di kayn ``antijen'' dεm we de de, kin εp fכ no if di sεl we dεn de kכwεshכn hεlty כ kεnsar.

If wi luk di minin fɔ di wɔd `Immunophenotyping`, wi kin ɛksplen dis mɔ:

• Imyun: Dis min sɔntin we gɛt fɔ du wit wi imyun sistɛm . insay dis tεst, dεn de yuz di protin dεm we dεn kכl ``Antigens'' we a bin tכk bכt fכ no כda kayn protin we dεn kכl ``Antibodies'' we de insay wi imyun sistεm bak. Dis tan lɛk ki ɛn lɔk. na wan patikyula ki (Antibody) we dεn mek fכ am nכmכ fit wan spεsifi k lכk (Antigen).

• Fεnotayp: Dis de tכk bכt di kכntribyushכn dεm we de na do, we yu kin si fכ sכmtin. So we dɛn tay da ki de (Antibody) to di lɔk (Antigen), wi kin si am wan patikyula we. we di patכlayz si dat, i no se di rilevεnt lכk, dat na di rilevεnt `(Antigen)`, de pan da sεl de.

dis `(Antigens)` (lכk) dεm we de fכm na di kεnsar sεl dεm difrεn frכm di wan dεm we de insay hεlty sεl dεm. So bay we yu no di kayn `(Antigens)` dεm εksεkt wan, dis tεst de εp fכ no wit sכri if dis na kεnsar sεl, εn if na so, us kayn kεnsar i bi.

Ustɛm dɛn fɔ du dis tɛst?

Bɔku tɛm yu dɔktɔ go tɛl yu fɔ du dis tɛst if yu kɔmplit blɔd kɔnt (CBC) sho se di wayt blɔd sɛl dɛn nɔ bɔku. Dis kin bi sayn fɔ sɔm blɔd kansa. so, di ``Immunophenotyping`` tεst de εp fכ kכnfכm da sכspεshכn de.

Dis we ya impɔtant bak fɔ du risach bɔt kansa. dis tεknכlכji dεn kin yus bak fכ stכdi aw kεnsar sεl dεm wit sכm ``Antigens`` de rεspכnd to difrεn anti-kansa dεm, spεshal wan ``Immunotherapy`` tritmεnt dεm.

Aw dɛn kin du di tɛst? Wetin kin apin insay di lab?

Insay dis tɛst, yu jɔs gɛt fɔ du wan rili smɔl tin. Dat na fɔ gi di sampul we dɛn nid fɔ du di tɛst. Afta dat, ɔl ɔda tin kin apin insay di lab. Yu nɔ nid fɔ wɔri bɔt am. Bɔku tɛm, dɛn kin yuz tu men we dɛn fɔ du dis tɛst.

1. Flɔ Saytometri

2. Imyunohistokemistri (IHC) .

Lɛ wi si wetin kin apin pan dɛn tu we ya.

Flɔ Saytometri Mɛtɔd

Dis na di we aw dɛn kin yuz mɔ. Dis we ya de mek dɛn ebul fɔ tɛst bɔku bɔku sɛl dɛn we dɛn tek frɔm blɔd ɔ tisu wan tɛm. Na dis na wetin de apin insay di lab:

• Fɔ tek di sampul: Dɛn kin du dis bay we dɛn tek smɔl sampul pan yu bon mɛro (bon mɛro bayɔpsi). Ɔ, mɔ, na bay we dɛn tek smɔl blɔd. sכmtεm, dεn kin tek sεmpl fכ spεnal fכluid כ abdominal fכluid bak.
• Fɔ kip di sampul: Insay di lab, dɛn kin pripia di sampul bay we dɛn ad spɛshal kemikal dɛn fɔ mek di sɛl dɛn nɔ pwɛl ɛn nɔ day.
• Add Antibody: Dɔn di pat we impɔtant pas ɔl de kam. dεn de ad wan ``Antibodi'' (ki) we dεn mek fכ mach di ``Antigen'' we wi de luk fכ to dis sεmpl. Bɔt sɔntin spɛshal de bɔt dis ``Antibody''. I gɛt smɔl kɔmpawnd we de mek layt, we na ``Fluorescent Compound'' we dɛn tay pan am. So we da ki de kɔnɛkt to di lɔk, da sɛl de bigin fɔ mek layt.
• insay di Flɔ Saytomita: afta dat dεn kin put dis sεmpl we dεn pripia insay wan spεshal mashin we dεn kכl ``Flow Saytomita.`` As di sεl dεm de muv tru dis mashin wan bay wan, di sεns dεm we de insay di mashin de dεtekt εn kכnt di sεl dεm we de mek brayt layt.

wit dis mכt, i posbul fכ rili akכrd fכ no aw mכl sεl dεm gεt di rilevεnt `(Antigen)`.

Imyunohistochεmistry (IHC) mεtכd

dis mεtכd dεn kin yus fכ fכn di `(Antigens)` we de insay wan spεsifi k tisu sεmpl `(Tissue Sample). Na ya, sɔntin we fiba `(Flow Cytometry)` de apin. bכt di big difrεns na dat ya, di `(Antibody)` nכ de atak fכ fכrosεnt kכmpawnd. insted, wan enzym `(Enzyme)` de atak.

biכs fכ dis εnzym, we di `(Antibody)` de tay to di rilevεnt `(Antigen)`, dat sεl de tכn wan spεsifi k kכla. Dɔn, we di dɔktɔ we de mɛn sik dɛn luk am wit maykroskɔp, i kin si dɛn sɛl dɛn de we gɛt kɔlɔ klia wan. da we de, i kin kכnfכm se di rilevεnt `(Antigen)` de insay da tisu de.

Ɛnitin de we pɔsin nɔ ebul fɔ du ɔ we i nɔ ebul fɔ du dis tɛst?

Yɛs, lɛk ɔl di tɛst dɛn, dis wan gɛt tin dɛn we i nɔ ebul fɔ du. Di big chalenj na dat nɔto ɔl lab we de du dis tɛst de fala di sem standad mɛtodɔlɔji. Dis min se smɔl difrɛns kin de pan di rizɔlt frɔm wan lab to ɔda wan.

Us kayn tin dɛn de kɔmɔt na di ripɔt? Wetin i se?

di ripot fכ `Immunophenotyping` tεst nכ simpul lεk rεgulεr blכd ripot. I gɛt data ɛn grafik dɛn we dɛn gɛt frɔm di `(Flow Cytometer)` mashin. Na pɔsin we tren fɔ mɛn sik dɛn nɔmɔ go ebul fɔ ɔndastand dɛn tin ya. I kin analayz da data de ɛn pripia di las ripɔt.

Frɔm da ripɔt de, yu dɔktɔ go gɛt infɔmeshɔn lɛk dis:

• Us kayn wayt blɔd sɛl de na di sampul?
• Us stej fכ divεlכpmεnt dεn sεl dεm de insay?
• כl di sεl dεm gεt di `(Antigen)` we dεn tεst?

Di patɔlɔjis kin kɔmpia di data frɔm yu sampul wit di data frɔm pɔsin we gɛt wɛlbɔdi, ɛn bak wit di data patɛn frɔm pipul dɛn we gɛt wan patikyula kayn lukimiya ɔ limfoma. Bay dis kɔmpiashɔn, wit di rizɔlt fɔ ɔda tɛst dɛm, i kin mek wan kɔnklushɔn bɔt di chans fɔ mek yu gɛt di kansa we i sɔspɛkt.

Ustɛm a fɔ tɔk to di dɔktɔ?

We yu wet fɔ di tɛst rizɔlt kin mek yu strɛs. Yu gɛt wan tawzin kwɛstyɔn dɛn na yu maynd. Na nɔmal tin fɔ fil se yu nɔr shɔ ustɛm di rizɔlt go kam ɛn wetin dɛn go tɔk.

Sɔntɛm yu nɔ de gɛt ɛni prɔblɛm, sɔntɛm yu sɔprayz fɔ si di `CBC` ripɔt. Sɔntɛm yu dɔn de fil taya smɔl ɛn nɔ wɛl fɔ sɔm tɛm. Bɔt, dis na tɛm fɔ peshɛnt. Dis na jɔs wan step pan di joyn fɔ no wetin de apin insay yu bɔdi.

Yu dɔktɔ na yu bɛst gayd dis tɛm.

• If yu nɔ shɔ ustɛm yu go gɛt di rizɔlt, aks yu dɔktɔ.
• Afta yu dɔn gɛt yu rizɔlt, if yu nɔ klia bɔt wetin de insay dɛn, aks kwɛstyɔn te yu ɔndastand. Dat na yu rayt.
• Nɔ kɔnfyus wit wetin de insay di ripɔt. Na di dɔktɔ in wok fɔ ɛksplen wetin i min fɔ yu.

Yu sɛl dɛn gɛt bɔku tin dɛn bɔt yu wɛlbɔdi. Immunophenotyping na jɔs wan tin wae kin ɛp yu fɔ no da infɔmeshɔn de ɛn gɛt dip ɔndastandin bɔt yu wɛl bɔdi. Na nɔmal tin fɔ fil fɔ fred ɛn wɔri na tɛm lɛk dis. If yu nid sɔpɔt, tɔk to yu dɔktɔ bɔt am.