An ka dɔn dɔrɔn Polyclonal Anticorps kan - kɛlɛbolo fitinin min bɛ an farikolo la

Ni farigan walima farigan donna an na, a bɛ kɛnɛya tile damadɔ kɔnɔ, tiɲɛ tɛ? Yala i delila k’i yɛrɛ ɲininka o bɛ kɛ cogo min na wa? Kɛlɛboloba dɔ fana b’an farikolo kɔnɔ, min bɛ banakisɛw kɛlɛ minnu bɛ bɔ kɛnɛma. Sɔrɔdasi kɛrɛnkɛrɛnnen minnu bɛ o kɛlɛbolo in na, olu dɔ bɛ Weele ko ‘anticorps’. Bi an bɛna kuma banakisɛfagalan suguya kɛrɛnkɛrɛnnen dɔ kan, n’o ye ‘Polyclonal Anticorps’ ye. Hali n’a y’a sɔrɔ nin tɔgɔ in mankan bɛ dɔnniya siratigɛ la dɔɔnin, maana in ka nɔgɔn kosɛbɛ. An ka nin farikolo tangalan ninnu kunnafoni kunbabaw lajɛ.

N’an y’a fɔ cogo nɔgɔman na, nin banakisɛfagalan poliklɔniw ye mun ye?

Banakisɛfagalanw (polyclonal anticorps) ye farikolojɔlan misɛnninw ye minnu bɛ an ka farikolo tangacogo dɔ la. Kɛrɛnkɛrɛnnenya la, u bɛ Bɔ farikolokisɛ kulu dɔ fɛ min bɛ Weele ko plasma B cellules.

Sisan an k’o nɔgɔya dɔɔnin. Miiri k’a filɛ i jugu dɔ nana i ka jamana na. Walasa k’o jugu dɔn, ‘taamaʃyɛn’ kɛrɛnkɛrɛnnen dɔw bɛ a fari la. O cogo kelen de bɛ kɛ ni banakisɛ, banakisɛ walima banakisɛ, donna an fari la. O tumu in bɛ ni ‘taamaʃyɛn’ kɛrɛnkɛrɛnnen ye a ɲɛda la. Furakɛcogo la, an bɛ o taamasiyɛn wele ko ‘antigène’ .

Ni an farikolo tangalanw ye nin antijeni in ye, a b'a dɔn k'a fɔ ko "Oh... nin tɛ an dɔ ye, kɛnɛma mɔgɔ don." O kɔ fɛ, an ka kɛlɛbolo, n’o ye banakisɛfagalanw ye, olu bɛ taa baara la.

Nka fɛn fitinin wɛrɛ bɛ yan. Antigène kelen kan, o kɔrɔ ye ko jugu kelen farikolo kan, yɔrɔ damadɔ bɛ yen an ka anticorps bɛ se ka minɛ minnu na. I n’a fɔ dingɛ damadɔ bɛ yen cogo min na, konnɛgɛw bɛ don da kelen kɔnɔ cogo min na. An bɛ o dingɛ kelen-kelen bɛɛ wele ko ‘epitope’ .

Daɲɛ ‘polyclonal’ kɔrɔ ye ‘ka Bɔ suguya wɛrɛw la’. O la, banakisɛfagalanw (polyclonal anticorps) ye farikolojɔlifɛn suguya caman dalajɛlen ye minnu bɛ se ka bin yɔrɔ wɛrɛw kan (epitopes) jugu kelen kan (antigène). O bɛ dɔ bɔ jugu ka ɲɛnamaya kɛcogo la kosɛbɛ.

Hali ni ninnu bɛ sɔrɔ an farikolo la u yɛrɛ la, walasa ka baara kɛ ni u ye furakɛli la, tuma dɔw la u bɛ bɔ baganw fari la i n’a fɔ laɲɔ ni saga, walima mɔgɔ minnu kɛnɛyara ka bɔ bana dɔw la, olu joli la.

Olu bɛ baara kɛ cogo di farikolo kɔnɔ?

O kɛcogo ka di kosɛbɛ. Miiri k’a filɛ i n’a fɔ kilisi ni tori.

1. Jugu bɛ don: Banakisɛ walima banakisɛ dɔ bɛ don an fari la. ‘Pigment’ bɛ a ɲɛda la. Olu ye antijeniw ye.

2. Toroko kunnafoni: Nka tori kelen-kelen bɛɛ bɛ ni kilisi dingɛ damadɔ ye. Olu ye epitɔpuw ye.

3. Kɛlɛbolow ka kɛlɛbolo bɛ se: Ni an farikolo tangalanw ye nin fɛnɲɛnama in ye dɔrɔn, a bɛ ‘kɔlɔsili suguya wɛrɛw’ caman dilan minnu bɛ don dingɛ kelen-kelen bɛɛ kɔnɔ. O kilisi kɛlɛbolo bɛ wele ko anticorps polyclonaux.

4. Kɛlɛ bɛ daminɛ:O kilisi suguya wɛrɛw (anticorps) bɛ siri dingɛ suguya wɛrɛw la (epitopes) jugu farikolo la. U bɛ bin jugu kelen kan ka bɔ yɔrɔ caman na.

5. A bɛ jugu tiɲɛ: Ni banakisɛfagalanw nɔrɔla nin cogo la, farikolo tangalan farikolokisɛ wɛrɛw bɛ se ka o jugu in dɔn nɔgɔya la ani k’a tiɲɛ.

Ninnu bɛ kɛ mun na furakɛli la?

Ka fara u dilanni kan an farikolo la, o banakisɛfagalan poliklɔniw bɛ kɛ baara nafama caman na furakɛli la.

• Sɛgɛsɛgɛli : Laboratowari sɛgɛsɛgɛli dɔw, i n’a fɔ ELISA ani immunohistochimie, olu bɛ kɛ ka banakisɛ walima kansɛri sɔrɔli dɔn.
• Dɔnniya ɲininiw kama: Sɛgɛsɛgɛlikɛlaw bɛ baara kɛ ni ninnu ye walasa k’a faamu an farikolo bɛ baara kɛ cogo min na ani banaw bɛ sɔrɔ cogo min na.
• Banaw furakɛli ni u kunbɛnni kama: Banakisɛfagalan caman bɛ kɛ ka bana dɔw furakɛ i n’a fɔ fura dɔw tali kojugu, sa ka kinni, ani joli bana dɔw.

Danfara jumɛn bɛ farikolo tangalan poliklɔni ni banakisɛfagalan kelen cɛ?

N’a sɔrɔ aw ye ‘banakisɛfagalan kelen-kelenw’ fana mɛn. Hali ni nin fila bɛɛ bɛ bin antigènes (banakisɛfagalanw) kan, danfara fitinin bɛ u ni ɲɔgɔn cɛ nka u nafa ka bon.

Nafa jumɛnw b’o la?

• A dilanni musaka ka dɔgɔn : Ninnu dilanni ka nɔgɔn ka tɛmɛ banakisɛfagalanw kan minnu bɛ kɛ ni fɛn kelen ye, olu bɛ dilan cogo gɛlɛn na laboratuwari kɔnɔ.
• Kɔlɔsili seko ɲuman : Hali ni antijeni hakɛ min bɛ sɛgɛsɛgɛlikɛlan dɔ la, o ka dɔgɔn kosɛbɛ , o fɛnw bɛ se k’a dɔn nɔgɔya la barisa a bɛ se ka siri yɔrɔw la minnu tɛ kelen ye.
• Kɛrɛnkɛrɛnnenya la kosɛbɛ: Ikomi banakisɛfagalan kelen bɛ siri yɔrɔ caman na, o la, jaabi bɛ kɛ cogo kelen na ka tɛmɛ.

Yala nafa foyi tɛ yen o tuma na wa?

Tiɲɛ don, nafa dɔw fana bɛ yen.

• Jogoɲumanya caman ɲɔgɔnna : Ikomi ninnu bɛ sɔrɔ u yɛrɛ la, jogo caman ɲɔgɔnna bɛ se ka kɛ banakisɛfagalanw kulu kelen ni kulu wɛrɛ cɛ. O la, a nafa bɛ se ka ɲɔgɔn ta.
• Fɛn minnu bɛ se ka kɛ sababu ye ka jaabi ɲuman nkalonmaw sɔrɔ: Tuma dɔw la, a bɛ se ka kɛ ko a bɛ se ka kɛ sababu ye ka ɲɔgɔn sɔrɔ ni antijeni wɛrɛ ye min ni antijeni laɲinilen bɛ tali kɛ ɲɔgɔn na. O la, jaabi nkalonmaw bɛ se ka sɔrɔ sɛgɛsɛgɛliw la.
• Farikoloɲɛnajɛ : Ni farikolojidɛsɛbanakisɛ minnu bɛ bɔ baganw na, olu kɛra ka hadamadenw furakɛ, faritanabana walima kɔlɔlɔ wɛrɛw farati bɛ a la.

O la, i n’a fɔ aw ɲɛ b’a la cogo min na, nin farikolo tangalan poliklɔni ninnu ye an farikolo farikolo tangacogo yɔrɔ nafamaba ye, ka fara furakɛli kan. Tuma caman na, u bɛ kɛ ɲininiw ni bana sɛgɛsɛgɛliw la. Ni aw bɛ ɲininkali dɔw kɛ o ko la, kɛrɛnkɛrɛnnenya la ni ninnu bɛ di aw ma furakɛli kɛrɛnkɛrɛnnen dɔ kama, a ka fisa aw ka dɔgɔtɔrɔ walima dɔgɔtɔrɔso baarakɛla ɲininka a ka ɲɛfɔli kɛ.