Maana dɔ an ka seliluw kan: An ka Flow Cytometry sɛgɛsɛgɛli dɔn cogo nɔgɔman na!

Yala aw delila ka sɛgɛsɛgɛli dɔ mɛn min tɔgɔ ye ko Flow Cytometry wa? Laala aw ka dɔgɔtɔrɔ ye nin sɛgɛsɛgɛli in fɔ aw ye. Walima n’a sɔrɔ o ye ko kura ye i bolo. Nka, o ye sɛgɛsɛgɛli ye min nafa ka bon kosɛbɛ, min b’an dɛmɛ ka kunnafoni caman sɔrɔ an farikolo yɔrɔw kan. Bi, an bɛna kuma o kan cogo nɔgɔman na, i bɛ se ka min faamu.

Flow Cytometry ye mun ye?

N’an y’a fɔ cogo nɔgɔman na, flow cytometry ye laboratuwari sɛgɛsɛgɛli ye min bɛ kɛ walasa ka kunnafoni dɔw sɔrɔ an farikolo kɔnɔ selilɛriw walima fɛn misɛnninw kan. Miiri k’a filɛ i n’a fɔ detekti fitinin dɔ min bɛ ka an ka kasobonw kɔnɔna lajɛ.

Walasa k’o kɛ, aw bɛ fɔlɔ ka aw ka farikolokisɛw (minnu bɛ se ka kɛ joli, kolotugudaw walima farikolo yɔrɔ wɛrɛw ye) bɛ wuli ji la. O kɔfɛ, laboratuwari baarakɛla dɔ bɛ kalali kɛrɛnkɛrɛnnenw fara farikolokisɛw kan minnu bɛ yeelen bɔ . O kalali ninnu bɛ farikolojɔlifɛn kɛrɛnkɛrɛnnenw dɔn ani ka nɔrɔ u la farikolokisɛw kan walima u kɔnɔ. O kɔrɔ ye ko u bɛ taamasiyɛn fitinin dɔ, walima ‘tag’ nɔrɔ o farikolojɔlifɛnw na. O kɛcogo bɛ wele tuma dɔw la ko Fluorescence-Activated Cell Sorting (FACS) .

O kɔfɛ, o selilu labɛnnenw bɛ don masin dɔ kɔnɔ min bɛ wele ko flow cytometer. Kabako don, ɔridinatɛri min sirilen bɛ nin masin in na, o bɛ se ka selilɛri 10 000 ɲɔgɔn sɛgɛsɛgɛ miniti kelen kɔnɔ! Yala o tɛ teliya kosɛbɛ wa?

O la sa, nin kɔrɔbɔli in bɛ kɛ mun na?

Dɔgɔtɔrɔw bɛ baara kɛ ni nin flow cytometry test ye fɛn caman na. Kɛrɛnkɛrɛnnenya la:

• Kɔnɔnatumu jate: Aw bɛ a dɔn tigitigi selilu hakɛ min bɛ misali dɔ kɔnɔ.
• Kɔnɔnatumuw tilatilali: Aw bɛ selilu suguya wɛrɛw faranfasi ani k’u dɔn.
• Selilu jogo ni a baarakɛcogo dɔnni: Aw bɛ fɛnw dɔn i n’a fɔ selilu cogoya ani a baaracogo.
• Joli ni kolotugudaw kansɛriw sɛgɛsɛgɛli n’u furakɛli : O nafa ka bon kosɛbɛ walasa ka banaw dɔn i n’a fɔ jolidɛsɛ ni jolidɛsɛ.
• Biyomarkɛriw sɔrɔli : Olu ye fɛɛrɛw ye minnu bɛ se ka kansɛri bɛna kɛ cogo min na ani a bɛna a jaabi cogo min na furakɛli la.
• Immunophenotyping (Immunophenotyping): Ka farikolo tangalan suguya wɛrɛw dɔn ani k’u tila.

Flow cytometry test bɛ kɛ tuma jumɛn?

Dɔgɔtɔrɔw bɛ baara kɛ ni o sɛgɛsɛgɛli ye walasa ka kunnafoni kunbabaw sɔrɔ an farikolo kɔnɔnatumuw kan. Kɛrɛnkɛrɛnnenya la:

• Aw ye kunnafoni sɔrɔ farikolo tangalanw kan: Aw ye bana kɛlɛli farikolokisɛ hakɛ lajɛ an farikolo la ani u suguya minnu ye.
• Aw bɛ kansɛribanakisɛw dɔn ani k’u cogoya faamuya: Aw bɛ a dɔn kansɛribanakisɛw ni farikolokisɛ tɔw tɛ kelen ye cogo min na ani u jogo ye min ye.
• Sɛgɛsɛgɛli kɛ furakɛli cogoya gɛlɛnw kan:
• Joli kansɛriw: Misali la, bana minnu bɛ i n’a fɔ jolidɛsɛ ni jolidɛsɛ.
• Jolilabana wɛrɛw : Misali la, bana dɔw i n’a fɔ paroxysmal nocturnal hemoglobinuria (PNH) ani antithrombine dɛsɛ.
• Banakisɛfagalanw ka baara : Aw bɛ banaw lajɛ i n’a fɔ sidabana (SIDA) ani farikolo tangacogo ciyɛntalen.

Fɛn wɛrɛ ye, tuma dɔw la, aw ka joli jate dafalen (CBC) kɛlen kɔfɛ, ni kunnafoni wɛrɛw ka kan ka kɛ ka da a kan, o bɛ se ka kɛ ka nin flow cytometry test in kɛ. Kɛrɛnkɛrɛnnenya la, ni CBC ye dɔ fara farikolokisɛ suguya dɔ kan min bɛ wele ko lanposotiw, ni fɛn dɔw bɛ farikolokisɛ wɛrɛw jate la, walima ni a y’a jira ko joli kuru kɔrɔbaliw bɛ yen, o sɛgɛsɛgɛli bɛ se ka yamaruya.

Nin sɛgɛsɛgɛli in nafa ka bon kosɛbɛ kansɛri sɛgɛsɛgɛli dɔrɔn tɛ, nka a nafa ka bon kosɛbɛ kansɛri furakɛli fana na. Nin don:

• Aw ye jateminɛ kɛ kansɛri ka juguya bɛ cogo min na.
• aw bɛ a dɔn ni kansɛri bɛna furakɛli kɛrɛnkɛrɛnnen dɔ jaabi .
• O fana bɛ dɛmɛ ka a dɔn ni kansɛri seginna ka kɛ furakɛli kɔfɛ.

Nin flow cytometry machine in bɛ baara kɛ cogo di? O ye kabako ye dɛ!

Nin flow cytometry machine in bɛ seliluw farikolo ni u ka kemikaliya cogoyaw sɛgɛsɛgɛ joona kosɛbɛ. Miiri k’a filɛ i n’a fɔ ka selilɛriw lajɛ kamera teliyalenba dɔ fɛ. A bɛ baara kɛ cogo min na, o filɛ nin ye:

1. Ka sɛgɛsɛgɛli kɛ : Fɔlɔ, laboratuwari baarakɛla bɛ aw joli, farikolo yɔrɔ walima kolotugudaw ta ka a wuli ji la (a bɛ wele ko `suspension` ). O kɔfɛ, i n’a fɔ a fɔra cogo min na ka tɛmɛ, kalali kɛrɛnkɛrɛnnen dɔ bɛ fara farikolokisɛw kan min bɛ wele ko `fluorescently tagged anticorps` . O kɔfɛ, selilabana labɛnnen bɛ don flow cytometer kɔnɔ.

2. Kɔnɔnatumuw labɛncogo ani laser-fiɲɛ: Masina kɔnɔ, o seliluw bɛ labɛn ɲɔgɔn fɛ, kelen-kelen. Miiri k’a filɛ, i n’a fɔ lakɔlidenw bɛ layini na. O kɔ, o seliw kulu in bɛ tɛmɛ laser-fiɲɛ dɔ ɲɛfɛ, kelen kelen. Ni laser-fiɲɛ ye seliw gosi, yeelen bɛ jɛnsɛn, o kɔrɔ ye ko yeelen jɛnsɛnnen.

3. Selilu jate ni u tilatilali : Flow cytometer bɛ selilu jate ani k’u tila ka kɛɲɛ ni laser yeelen bɛ yeelen jɛnsɛn cogo min na ni a ye seliw gosi. Kɔnɔnatumu suguya kelen-kelen bɛɛ bɛ yeelen jɛnsɛn cogo wɛrɛ la. Masina b’o kɛ a kɛtɔ k’o danfara dɔn.

4. 4. .Donanw ka taa ɔridinatɛri la: Kunnafoni (dantigɛli) minnu bɛ lajɛ masin fɛ o cogo la, olu bɛɛ bɛ ci ɔridinatɛri ma min ni a cɛsirilen don.

5. Rapport dilancogo: Ɔridinatɛri bɛ o kunnafoniw sɛgɛsɛgɛ ka rapɔɔri dɔ dilan, a ka c’a la, a bɛ kɛ dot walima bar graph cogo la .

6. Banakisɛfagalan ka sɛgɛsɛgɛli : A laban na, banakɔtaa dɔnbaga dɔ – dɔgɔtɔrɔso kɛrɛnkɛrɛnnen min bɛ banaw sɛgɛsɛgɛ – o bɛ o ja ninnu ni kunnafoniw sɛgɛsɛgɛ, ka sɛbɛn dɔ fara sɛgɛsɛgɛli rapɔɔri kan, a ka sɔrɔw ni a ka dantigɛliw bɛ min kɔnɔ.

Ne ka kan ka mun kɛ sani ka flow cytometry test kɛ? Yala fɛn dɔ bɛ yen min ka kan ka siran wa?

A ka ca a la, fo ni dɔgɔtɔrɔ ye cikan kɛrɛnkɛrɛnnenw di aw ma, labɛn kɛrɛnkɛrɛnnen si man kan ka kɛ sani nin flow cytometry sɛgɛsɛgɛli in ka kɛ.

Aw ka dɔgɔtɔrɔ mago bɛna kɛ joli, farikolo yɔrɔ walima kolotugudaw la walasa ka nin sɛgɛsɛgɛli in kɛ. A ka ca a la, nin sɛgɛsɛgɛli in bɛ se ka ta aw ka dɔgɔtɔrɔso la aw ka taa bɔ waati la. Sɛgɛsɛgɛli kɛlen kɔfɛ, a bɛ ci laboratuwari dɔ la walasa ka sɛgɛsɛgɛli kɛ.

Ni banakɔtaa dɔnbaga ye flow cytometry sɛgɛsɛgɛli kɛ ka ban, a bɛna kunnafoniw sɛgɛsɛgɛ ani ka kunnafoni ci aw ka dɔgɔtɔrɔ ma min ye sɛgɛsɛgɛli ɲini.

Min nafa ka bon kosɛbɛ, o ye ko farati dɔntaw tɛ aw la sisan ni nin flow cytometry test in kɛra. O la sa, aw kana siran nin ɲɛ kun tɛ.

O nɔ bɛ kɛ cogo di? U bɛ mun fɔ an ye?

A ka ca a la, a bɛ tile damadɔ fo dɔgɔkun kelen kɛ walasa ka flow cytometry test jaabi sɔrɔ, ka kɛɲɛ ni laboratuwari ye min bɛ kɛ aw ka dɔgɔtɔrɔ fɛ.

Banakisɛfagalan bɛ taamasiɲɛ minnu lajɛ i ka farikolokisɛw sanfɛ, olu bɛ wele ko antigènes .

• Kɔnɔnatumu kɛnɛman bɛ ni antijeni kɛcogo ɲuman ye min bɛ bɛn o selilɛri suguya ma ani a yiriwali taabolo ma.
• o cogoya tɛ kelen ye selilɛri la min tɛ cogo la . Yɛlɛma ninnu bɛ se ka a jira ko jolidɛsɛ, jolidɛsɛ, walima furakɛli bana wɛrɛw bɛ yen.

Ni aw ka dɔgɔtɔrɔ ye sɛgɛsɛgɛli rapɔɔri sɔrɔ, a bɛna banabagatɔ ka sɛbɛn lajɛ ka ɲɛ, o min ye kunnafoniw lajɛlen ye minnu sɛgɛsɛgɛra ɔridinatɛri fɛ ka bɔ flow cytometer la.

Ni aw bɛ aw ka flow cytometry jaabiw kɔrɔ fɔ, aw ka dɔgɔtɔrɔ bɛna aw ka bana taamasiɲɛw jateminɛ, aw ka kɛnɛyako tariku, ani farikolo sɛgɛsɛgɛli min kɛra kɔsa in na . A bɛna o nɔw ɲɛfɔ i ye ani k’u kɔrɔ ɲɛfɔ i ye. A bɛna baara kɛ ni o kunnafoniw ye fana walisa k’a latigɛ a bɛna min kɛ kɔfɛ.

Bana sugu jumɛnw de bɛ se ka kɛ ni kɔlɔlɔw ye minnu tɛ bɛn?

Ni a nɔfɛkow ma kɛ cogo la, o bɛ se ka bana dɔw jira i n’a fɔ:

• Jolidɛsɛ jugumanba min bɛ wele ko lymphoblastique
• Jolidɛsɛ miyɛloyi jugumanba
• Jolidɛsɛ basigilen min bɛ sɔrɔ lanposoti fɛ
• Miyɛloma caman
• Banakisɛfagalan minnu tɛ Hodgkin ye (o bɛ se ka kɛ B-cell ni T-cell suguya fila bɛɛ ye) .

Ni o nɔw ma kɛ cogo la, mun bɛ kɛ o kɔfɛ?

Aw ka dɔgɔtɔrɔ bɛna baro kɛ aw fɛ ni aw ka flow cytometry jaabiw ye ani furakɛli suguya minnu bɛ se ka kɛ. Nin sɛgɛsɛgɛli rapɔɔri bɛna aw ka dɔgɔtɔrɔ dɛmɛ a k’a faamu aw ka bana bɛ juguya cogo min na, ni furakɛli kɛrɛnkɛrɛnnen dɔ bɛnnen don aw ma, ani ni a ka ca a la ko aw ka kansɛri bɛ segin furakɛli kɔfɛ . A bɛna baara kɛ ni nin kunnafoniw bɛɛ ye walasa ka furakɛli bolodacogo dɔ labɛn min bɛ bɛn aw ma.

Bana dɔw bɛ waati dɔ ta ka sɔrɔ ka u sɛgɛsɛgɛ. N’a sɔrɔ i ye sɛgɛsɛgɛli caman kɛ, nka hali bi i ma jaabi ɲuman sɔrɔ, wa i bɛ se k’i yɛrɛ ɲininka ko: “Ni ne ye sɛgɛsɛgɛli wɛrɛ kɛ do?” Nka aw ka da a la, nin flow cytometry test in bɛ se ka kunnafoni nafama caman di aw ka dɔgɔtɔrɔ ma aw farikolo kɔnɔ farikolokisɛw kan. A tɛna dɛmɛ dɔrɔn ka bana in sɛgɛsɛgɛ, nka a bɛna aw dɛmɛ fana ka furakɛli bolodacogo dɔ labɛn min bɛ kɛ aw yɛrɛ ta ye, min bɛ kɛ aw yɛrɛ ta ye.

O la sa, an ka kan ka an hakili to fɛn jumɛnw na nin bɛɛ la? (Take-Home Message)

O bɛ ɲɛ, a to n ka hakilina nafama dɔw lajɛ ka bɔ an ye kuma minnu kan:

• Flow cytometry ye sɛgɛsɛgɛli ye min ka bon kosɛbɛ, min bɛ seliluw sɛgɛsɛgɛ ka ɲɛ.
• O bɛ dɛmɛ ka joli kansɛriw, farikolo tangacogo gɛlɛyaw, ani bana damadɔ wɛrɛw sɛgɛsɛgɛ .
• A ka c’a la, o sɛgɛsɛgɛli bɛ kɛ ni joli, kolotuguda walima farikolo yɔrɔ dɔ ta ye .
• Nin sɛgɛsɛgɛli in tɛ labɛn caman wajibiya sani a ka kɛ, wa farati foyi tɛ a la.
• O jaabiw bɛ kunnafoni nafamaw di dɔgɔtɔrɔ ma i ka bana kan, furakɛli bɛnnenw kan, ani bana bɛna taa ɲɛ cogo min na .
• nin ye baarakɛminɛn nafamaba ye min bɛ dɔgɔtɔrɔ dɛmɛ ka furakɛli bolodacogo dɔ labɛn min bɛ bɛn aw ma .

O la, ni dɔgɔtɔrɔ dɔ ye flow cytometry sɛgɛsɛgɛli fɔ aw ye walima aw dɔnbaga dɔ ye, aw kana siran. Aw ye a lajɛ iko fɛɛrɛ min bɛ se ka aw ka kɛnɛya faamuya ka ɲɛ ani ka aw dɛmɛ ka furakɛli ɲuman sɔrɔ. Ni ɲininkali dɔw bɛ aw fɛ, aw bɛ dɔgɔtɔrɔ ɲininka.

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` Flow cytometry, selilɛri sɛgɛsɛgɛli, joli kansɛri, bana sɛgɛsɛgɛli, farikolo tangacogo, furakɛli sɛgɛsɛgɛliw, selilɛri sɛgɛsɛgɛli