Immunostaining ye mun ye? An ka kuma nin banaw dɔnni sɛgɛsɛgɛli in kan daɲɛ nɔgɔmanw na!

Yala aw delila ka dɔgɔtɔrɔ dɔ bila ka sɛgɛsɛgɛli kɛ min tɔgɔ ye ko "Immunostaining" wa? Walima i ye o kumasen mɛn yɔrɔ dɔ la wa? N’a sɔrɔ a mankan ka gɛlɛn dɔɔnin i bolo. Nka aw kana aw hakili ɲagami, bi an bɛna kuma nin sɛgɛsɛgɛli in kan min bɛ wele ko Immunostaining, a bɛ min kɛ, a bɛ kɛ cogo min na, fɛn bɛɛ bɛ cogo nɔgɔman na kosɛbɛ i bɛ se ka min faamuya. I n’a fɔ i bɛ kuma i terikɛ fɛ cogo min na.

Immunostaining ye mun ye tigitigi?

N’an y’a fɔ cogo nɔgɔman na, farikolo tangacogo ye sɛgɛsɛgɛli kɛrɛnkɛrɛnnen ye min bɛ kɛ an ka banakɔtaa dɔnbagaw fɛ. A bɛ kɛ ka farikolojɔli dumuni kɛrɛnkɛrɛnnen dɔ dɔn farikolojɔlan caman cɛma, min bɛ sɔrɔ i farikolo la, i n’a fɔ joli, kolotugudaw walima farikolo yɔrɔ. Miiri k’a filɛ i n’a fɔ ka mɔgɔ kɛrɛnkɛrɛnnen dɔ sɔrɔ jama cɛma. Nin fɛɛrɛ in bɛ mɔgɔ dɛmɛ kosɛbɛ bana suguya caman sɛgɛsɛgɛli la, kɛrɛnkɛrɛnnenya la banakisɛw ni kansɛri.

Nin kɔrɔbɔli in bɛ baara kɛ cogo di?

O bɛ ɲɛ, sisan an k’a lajɛ nin immunostaining in bɛ baara kɛ cogo min na. A bɛ baara kɛ ni fɛn ye min bɛ wele ko banakisɛfagalanw . O farikolo tangalanw bɛ i n’a fɔ sɔrɔdasi minnu bɛ an farikolo lafasali la. U bɛ fɛnw dɔn ani ka nɔrɔ u la minnu bɛ bana bila mɔgɔ la, n’olu bɛ wele ko antigènes . O la, nin sɛgɛsɛgɛli in na, dɔgɔtɔrɔw bɛ baara kɛ ni farikolojɔlifɛn dɔ ye min kɛrɛnkɛrɛnnen don o farikolojɔlifɛn (min bɛ wele ko antigène) ma, u bɛ min ɲini.

O kɔ fɛ, "staining" yɔrɔ bɛ Nà. A bɛ iko i bɛ kumasen nafama dɔ jira gafe kɔnɔ. Nka a man nɔgɔn i n’a fɔ ka T-shirt kulɛri kɛ. O "nɔgɔbɔli" fɛɛrɛw bɛ baara kɛ ni kalali kɛrɛnkɛrɛnnenw ye, anzimu minnu bɛ kulɛri Changer, fluorescent molecules, ani hali sanu fɛnɲɛnamafagalanw. Olu bɛ farikolojɔlifɛnw ni farikolokisɛ minnu ɲini, olu bɛ taamasiyɛn kɛ, o la ɲininikɛlaw bɛ se k’u ye ka jɛya mikroskɔpu fɛ.

Sani aw ka nin farikolo tangalan sɛgɛsɛgɛli in kɛ, dɔgɔtɔrɔ bɛna aw joli, aw kolotugudaw walima aw farikolo yɔrɔ dɔ ta. O kɔfɛ, a bɛna ci banakɔtaa dɔnbaga dɔ ma walasa a ka sɛgɛsɛgɛli kɛ. Aw ye aw hakili to a la ko a kɛra dɔgɔtɔrɔso la wo, a kɛra dɔgɔtɔrɔso la wo, nin farikolo tangalan sɛgɛsɛgɛli in bɛ kɛ tuma bɛɛ laboratuwari la, walima an bɛ min wele ko laboratuwari.

Immunostaining suguya jumɛnw ye?

Farikolo tangacogo damadɔ bɛ yen dɔgɔtɔrɔw bɛ minnu kɛ. Fɛɛrɛ kelen-kelen bɛɛ bɛ ni sɛgɛsɛgɛli kɛcogo kɛrɛnkɛrɛnnen ye, walima protocole, min bɛ tali kɛ a la. Fɛɛrɛba minnu bɛ kɛ, olu dɔw filɛ nin ye:

• Immunohistochimie (banakisɛfagalanw ka farikolo tangacogo).
• Flow cytométrie (banakisɛfagalan min bɛ wele ko cytométrie).
• Immuno-électron microscopie (EM immunolabeling) .

Yala an bɛna o kelen-kelen bɛɛ lajɛ ka ɲɛ dɔɔnin wa?

Immunohistochimie - o ye fɛɛrɛ ye min bɛ kɛ ka caya

nin ye farikolo tangacogo ye min bɛ kɛ ka caya . Immunohistochimie la, a yɔrɔ fitinin dɔ bɛ bila galasi sɔgɔlen dɔ kan, ka sɔrɔ ka banakisɛfagalan dɔ fara a kan min bɛ siri laɲini na. O kɔfɛ, a bɛ nɔgɔ ni kalali kɛrɛnkɛrɛnnenw walima anzimu kɛrɛnkɛrɛnnenw ye. Olu bɛ wele ko immunostains. O b’a to banakɔtaa dɔnbaga bɛ se ka farikolojɔli dumuniw ni farikolokisɛ dɔw ye ka jɛya kosɛbɛ mikroskɔpu fɛ.

Aw ye a lajɛ farikolo tangalanw nafa ye minnu ye:

• A bɛ dɛmɛ ka kansɛri suguya wɛrɛw dɔn.
• A bɛ dɛmɛ ka hakiliɲagami bana caman ni jolilabanaw tila ɲɔgɔn na.
• O fana b’a to dɔgɔtɔrɔw bɛ se ka jamu dɔw nɔw, walima u jiracogo sɛgɛsɛgɛ.

Flow cytometry - i n’a fɔ masin min bɛ seliluw jate ani ka u faranfasi

Fɛɛrɛ dɔ la min bɛ wele ko flow cytometry, fɛɛrɛtigi dɔ bɛ baara kɛ ni farikolo tangalanw ye minnu bɛ ni taamasiɲɛ kulɛrilenw ye walasa ka taamasiɲɛ kɛ u bɛ farikolojɔli dumuni minnu ɲini. O kɔfɛ, laser-fiɲɛ kɛrɛnkɛrɛnnen dɔ bɛ kɛ ka i ka seliluw jate, k’u tila ɲɔgɔn na, k’u faranfasi kelen kelen. A bɛ i n’a fɔ ka dumunifɛnw sɛgɛsɛgɛ suguba kɔnɔ.

Dɔgɔtɔrɔw bɛ baara kɛ ni flow cytometry ye walasa ka banaw sɛgɛsɛgɛ i n’a fɔ:

• Banakisɛfagalanw
• Bana minnu bɛ sɔrɔ farikolo tangalanw fɛ
• Jolidɛsɛbana
• Linfoma (Linfoma) bɛ sɔrɔ

Immuno-électron microscopie (EM immunolabeling) .

Immuno-électron microscopie bɛ a to banakɔtaa dɔnbaga bɛ se k’i ka sɛgɛsɛgɛli kɛ ni bonyaba ye ani ka a ɲɛfɔ ka ɲɛ kosɛbɛ. O la, banakɔtaa dɔnbagaw bɛ sanu misɛnninw siri farikolojɔlanw na minnu bɛ wele ko sanu kolosinsinnanw . O sanu kolomalen in yecogo ka nɔgɔn kosɛbɛ ɛlektrɔniki mikroskɔpu fɛ. O la, ni o banakisɛfagalan taara banakisɛfagalan minɛ, sanufɛnw b’a to a bɛ ye ka jɛya i n’a fɔ nɛrɛmuguma.

Ka fara banakisɛfagalanw ni bana suguya caman dɔnni kan, farikolo tangalanw ni ɛlektrɔniki mikroskɔpi bɛ se kaHali ka banakisɛfagalanw sɔrɔ bolociw la.

Ne ka kan ka n yɛrɛ labɛn nin kɔrɔbɔli in kama cogo di?

A ka ca a la, aw man kan ka aw labɛn farikolo tangalanw sɛgɛsɛgɛli kama. Nka, dɔgɔtɔrɔ bɛna laadilikanw di aw ma sani aw ka aw joli, farikolo yɔrɔ walima kolotugudaw ta. O bɛna i dɛmɛ k’a dɔn tigitigi i bɛ se ka min makɔnɔ. A nafa ka bon ka dɔgɔtɔrɔ ka kuma lamɛn ka ɲɛ.

Kɔrɔbɔli bɛ waati joli ta? A bɛ waati joli ta walasa a nɔfɛkow ka segin?

O bɛ bɔ tiɲɛ na farikolo tangacogo la i ka dɔgɔtɔrɔ bɛ min kɛ. fɛɛrɛ dɔw bɛ sanga damadɔ dɔrɔn de ta , dɔw bɛ se ka tile 10 kɛ . Aw ka dɔgɔtɔrɔ bɛna se k’a fɔ aw ye a bɛna waati hakɛ min ta ka kɛɲɛ ni aw ka ko ye.

Waati min bɛ ta walasa jaabiw ka segin ka na, o fana bɛ o cogo kelen na. Banakisɛfagalanw tali fɛɛrɛ dɔw bɛ se ka nɔ di aw ma tile kelen kɔnɔ. Dɔ wɛrɛw bɛ se ka dɔgɔkun kelen walima ka tɛmɛ o kan. Ni aw taara sɛgɛsɛgɛli kɛ, aw ka dɔgɔtɔrɔ bɛna aw ladɔnniya waati min na aw bɛ se ka a nɔ makɔnɔ.

O la, nin waati in ɲɔgɔn na, a ka fisa aw ka muɲu, aw kana siran. Aw bɛ kuma dɔgɔtɔrɔ fɛ ka fɛn bɛɛ ɲɛfɔ.

O jaabiw bɛ mun fɔ? Mun bɛ kɛ ni u tɛ cogo la?

Banakisɛfagalan bɛna aw ka sɛgɛsɛgɛli jaabiw sɛgɛsɛgɛ ka u ci aw ka dɔgɔtɔrɔ ma. Aw bɛ jaabi minnu sɔrɔ, olu bɛ se ka ɲɔgɔn ta ka kɛɲɛ ni banakisɛfagalan ye sɛgɛsɛgɛli suguya min kɛ. Aw ka dɔgɔtɔrɔ bɛna aw ka sɛgɛsɛgɛli jaabiw ɲɛfɔ ka ɲɛ ani u kɔrɔ ye min ye aw ka ko kɛrɛnkɛrɛnnen na.

Aw ye aw hakili to a la ko ni sɛgɛsɛgɛli jaabiw ma kɛ cogo la , o kɔrɔ ye ko banakɔtaa dɔnbaga ye bana taamasiɲɛw sɔrɔ, aw kana siran. Aw ka dɔgɔtɔrɔ bɛna o nɔw ɲɛfɔ aw ye ani ka baro kɛ aw ni aw ka furakɛli suguyaw kan. Tuma dɔw la, dɔgɔtɔrɔw ka kulu dɔ bɛ se ka jɛ ka furakɛli kɛcogo ɲuman labɛn.

A laban na, fɛn minnu nafa ka bon kosɛbɛ, an ka kan ka minnu to an hakili la

Okey, donc ka bɔ an ye baro kɛ min kan, n jigi b’a kan ko aw ye hakilina ɲuman sɔrɔ immunostaining kan. Fɛn nafama dɔw filɛ nin ye minnu ka kan ka to an hakili la:

• Immunostaining ye laboratuwari sɛgɛsɛgɛli ye min bɛ kɛ ni farikolo tangalanw ye walasa ka banakisɛfagalan kɛrɛnkɛrɛnnenw dɔn ani ka u taamasiyɛn an ka farikolokisɛw kɔnɔ.
• Nin sɛgɛsɛgɛli in bɛ se ka dɛmɛ don ka kɛnɛyako suguya caman sɛgɛsɛgɛ, i n’a fɔ banakisɛw ni banakisɛw, ani kansɛri suguya dɔw.
• Ni aw ka kan ka farikolo tangalan sɛgɛsɛgɛli kɛ, kun t’a la ka siran.Aw ka dɔgɔtɔrɔ bɛna a ɲɛfɔ aw ye ka ɲɛ.
• Ni sɛgɛsɛgɛli jaabiw kɛra ka ban, aw bɛ kuma aw ka dɔgɔtɔrɔ fɛ kɛnɛ kan, u kɔrɔ ye min ye aw ka bana na ani ni furakɛli ka kan ka kɛ walima ni a man kan.

I hakili to a la ko furakɛli dɔnniya bɛ ka wuli tuma bɛɛ. Nin ɲɔgɔnna sɛgɛsɛgɛliw de fɛ an bɛ se ka bana caman dɔn joona, k’u furakɛ ka ɲɛ. O la, ni dɔgɔtɔrɔ dɔ ye o sɛgɛsɛgɛli sugu fɔ, a nafa ka bon ka i labɛn o kama.

` Immunostaining, Pathology, Anticorps, Antigènes, Kansɛri sɛgɛsɛgɛliw, Laboratowari sɛgɛsɛgɛliw