Èse dodokpɔ aɖe si woyɔna be Flow Cytometry ŋu nya kpɔa? Ðewohĩ wò ɖɔkta gblɔ dodokpɔ sia ŋu nya na wò. Alo ɖewohĩ enye nu yeye na wò. Ke hã, enye dodokpɔ vevi aɖe ŋutɔ si kpena ɖe mía ŋu míenyaa nyatakaka geɖe tso lãmenugbagbevi siwo le míaƒe ŋutilã me ŋu. Egbea, míaƒo nu tso eŋu le mɔ bɔbɔe aɖe si gɔme nàte ŋu ase nu.

Nukae nye Flow Cytometry?

Kpuie ko la, flow cytometry nye dodokpɔ si wowɔna le nudokpɔƒe si wozãna tsɔ nyaa nu tso lãmenugbagbeviwo alo nu suesuesue siwo le míaƒe ŋutilã me ŋu tsitotsito. Bu eŋu abe ʋɔnudrɔ̃la sue aɖe si le ŋku lém ɖe míaƒe gaxɔwo me ene.

Be nàte ŋu awɔ esia la, gbã la, wotsɔa wò lãmenugbagbeviwo (siwo ate ŋu anye ʋu, ƒumeŋuɖui, alo lãkusi bubuwo) ƒe kpɔɖeŋu aɖe ƒua tsii. Emegbe, dodokpɔxɔ me dɔwɔla aɖe tsɔa amadede tɔxɛ siwo nye fluorescent antibody dea lãmenugbagbeviawo me. Amadede siawo dea dzesi protein tɔxɛ siwo le lãmenugbagbeviwo dzi alo le wo me heléna ɖe wo ŋu. Ne míagblɔe bubui la, wotsɔa ŋkɔ sue aɖe, alo ‘tag’ dea protein mawo ŋu. Woyɔa dɔ sia ɣeaɖewoɣi be Fluorescence-Activated Cell Sorting (FACS) .

Emegbe wotsɔa lãmenugbagbevi siawo siwo wodzra ɖo la dea mɔ̃ aɖe si woyɔna be flow cytometer me. Nusi wɔ nukue nye be kɔmpiuta si wotsɔ do ka kple mɔ̃ sia ate ŋu adzro lãmenugbagbevi siwo ade 10,000 me le aɖabaƒoƒo ɖeka pɛ ko me! Ðe ema menye kabakaba ŋutɔ oa?

Eyata nukae wozãa dodokpɔ sia na?

Ðɔktawo zãa flow cytometry dodokpɔ sia hena nu geɖe. Vevietɔ:

• Lãmenugbagbeviwo xexlẽ: Kpɔ lãmenugbagbevi agbɔsɔsɔme si tututu le kpɔɖeŋu ɖeka me ɖa.
• Lãmenugbagbeviwo ƒe hatsotsowo me toto: Ma lãmenugbagbevi ƒomevi vovovowo ɖe vovo eye nàde dzesi wo.
• Lãmenugbagbevi ƒe nɔnɔme kple dɔwɔwɔ nyanya: Di nuwo abe lãmenugbagbevi ƒe nɔnɔme kple alesi wòwɔa dɔe ene.
• Ʋu kple ƒumeŋuɖui me dɔdzẽwo didi kple wo dada: Esia le vevie ŋutɔ be woade dzesi dɔlélewo abe ʋumenugbagbeviɣidɔ kple ʋumenugbagbeviɣidɔ ene.
• Biomarkers didi: Esiawo nye afɔɖeɖe siwo ate ŋu agblɔ alesi kansa awɔ nui kple alesi wòawɔ nui ne ewɔ atike nɛ.
• Dɔlélenutsiŋutete ƒe nɔnɔme: De dzesi dɔlélenutsiŋutete ƒe lãmenugbagbevi vovovo siwo le míaƒe ŋutilã me ahade wo hatsotsowo me.

Ɣekaɣie wowɔa flow cytometry dodokpɔa?

Ðɔktawo zãa dodokpɔ sia tsɔ xɔa nyatakaka deto tso lãmenugbagbevi siwo le míaƒe ŋutilã me ŋu. Etɔxɛe:

• Srɔ̃ nu tso dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ŋu: Kpɔ lãmenugbagbevi agbɔsɔsɔme si tsia tre ɖe dɔléle ŋu le míaƒe ŋutilã me kple esiwo ƒomevi wonye ɖa.
• De dzesi kansa lãmenugbagbeviwo eye nàse woƒe nɔnɔme gɔme: Kpɔ alesi kansa lãmenugbagbeviwo to vovo tso lãmenugbagbevi bubuwo gbɔ kple nusiwo nye woƒe nɔnɔmewo ɖa.
• Ku nu me tso atikewɔwɔ me nɔnɔme sesẽwo ŋu:
• Ʋu me kansa: Le kpɔɖeŋu me, dɔlélewo abe ʋumenugbagbeviɣidɔ kple ʋumenugbagbeviɣidɔ ene.
• Ʋumedɔléle bubuwo: Le kpɔɖeŋu me, nɔnɔmewo abe paroxysmal nocturnal hemoglobinuria (PNH) kple antithrombin ƒe anyimanɔmanɔ ene.
• Dɔlélenutsiŋutete ƒe dɔwɔwɔ: Kpɔ dɔlélewo abe AIDS (HIV) kple dɔlélenutsiŋutete ƒe gbɔdzɔgbɔdzɔ siwo ƒe dome nèle ɖa.

Nu bubue nye be, ɣeaɖewoɣi ne wowɔ wò ʋu ƒe xexlẽme bliboa (CBC) vɔ la, ne ehiã be woanya nyatakaka geɖe wu le emetsonuwo nu la, woate ŋu awɔ flow cytometry dodokpɔ sia. Etɔxɛe la, ne CBC ɖe lãmenugbagbevi ƒomevi aɖe si woyɔna be lymphocytes ƒe dzidziɖedzi fia, ne lãmenugbagbevi bubuwo ƒe xexlẽme mesɔ o, alo ne eɖe ʋumenugbagbevi siwo metsi o ƒe anyinɔnɔ fia la, woate ŋu adɔ dodokpɔ sia.

Dodokpɔ sia le vevie ŋutɔ menye na kansa didi ɖeɖeko o, ke le kansa dada hã me. Ema nye:

• Kpɔ alesi kansa la ƒe adãwɔwɔ le ɖa.
• Kpɔe ɖa be kansa la awɔ nu ɖe ​​atikewɔwɔ aɖe koŋ ŋu hã .
• Esia kpena ɖe ame ŋu hã be woanya ne kansa la gadze le atikewɔwɔ vɔ megbe.

Aleke flow cytometry mɔ̃ sia wɔa dɔe? Ewɔ nuku ŋutɔ!

Mɔ̃ sia si léa ŋku ɖe lãmenugbagbeviwo ƒe lãmenugbagbeviwo ŋu la léa ŋku ɖe lãmenugbagbeviwo ƒe dzɔdzɔmeŋusẽ kple atike ƒe nɔnɔmewo ŋu kabakaba ŋutɔ. Bu eŋu abe alesi nèle lãmenugbagbeviwo kpɔm to fotoɖemɔ̃ si zɔna kabakaba ŋutɔ dzi ene. Ale si wòwɔa dɔe nye esi:

1. Dzadzraɖo ɖe kpɔɖeŋua ŋu: Gbã la, dodokpɔxɔa ƒe mɔ̃ɖaŋudɔwɔla xɔa wò ʋu, lãkusi, alo ƒumeŋuɖui ƒe kpɔɖeŋu eye wòlɔnɛ ɖe tsi aɖe me (si woyɔna be `suspension` ). Emegbe abe alesi míegblɔe va yi ene la, wotsɔa amadede tɔxɛ aɖe si woyɔna be `fluorescently tagged antibodies` dea lãmenugbagbeviawo me. Emegbe wotsɔa lãmenugbagbeviwo ƒe tsi si wodzra ɖo la dea sisi ƒe lãmenugbagbeviwodzidzenu me.

2. Lãmenugbagbeviwo ƒe ɖoɖowɔwɔ kple laser-ya: Le mɔ̃a me la, woɖoa lãmenugbagbevi siawo ɖe fli ɖeka me, eye woɖo wo nɔewo ɖe wo nɔewo yome. Bu eŋu kpɔ, abe sukuvi siwo le fli me ene. Emegbe, lãmenugbagbeviwo ƒe fli sia toa laser-ya aɖe ŋgɔ, ɖekaɖeka. Ne laser-ya la dze lãmenugbagbeviawo dzi la, kekelia kaka, si fia be kekeli si kaka.

3. Lãmenugbagbeviwo xexlẽ kple wo me toto: Flow cytometer xlẽa lãmenugbagbeviwo eye wòdaa wo ɖe hatsotsowo me le alesi laser-ya kakaa kekeli ne edze lãmenugbagbeviawo dzi nu. Lãmenugbagbevi ƒomevi ɖesiaɖe kaka kekeli le mɔ vovovowo nu. Mɔ̃a wɔa esia to vovototo ma kpɔkpɔ me.

4. 4. .Nyatakakawo yi kɔmpiuta dzi: Woɖoa nyatakaka (nyatakaka) siwo katã mɔ̃a ƒo ƒu to mɔ sia nu la ɖe kɔmpiuta si do ƒome kplii.

5. Nyatakakawo wɔwɔ: Kɔmpiutaa dzroa nyatakaka siawo me eye wòwɔa nyatakaka, zi geɖe la, ewɔa nyatakaka si nye dot alo bar graph .

6. Dɔléleŋutinunyala ƒe numekuku: Mlɔeba la, dɔléleŋutinunyala – atikewɔla bibi si dea dzesi dɔlélewo – dzroa nɔnɔmetata kple nyatakaka siawo me eye wòtsɔa nuŋlɔɖi si me eƒe nusiwo ŋu wòke ɖo kple nyataƒoƒo le kpena ɖe dodokpɔa ƒe nyatakakaa ŋu.

Nukae wòle be mawɔ hafi awɔ flow cytometry dodokpɔ? Ðe nane li si ŋu wòle be woavɔ̃ na?

Zi geɖe la, negbe ɖe wò ɖɔkta na mɔfiame tɔxɛwo wò hafi la, dzadzraɖo tɔxɛ aɖeke mehiã hafi nàdo sisi ƒe lãmenugbagbeviwo ƒe dodokpɔ sia o.

Wò ɖɔkta ahiã ʋu, lãkusi, alo ƒumeŋuɖui ƒe kpɔɖeŋu hafi ate ŋu awɔ dodokpɔ sia. Zi geɖe la, woate ŋu axɔ kpɔɖeŋu sia le wò ɖɔkta ƒe dɔwɔƒe le wò sasrãkpɔ me. Ne woɖe kpɔɖeŋua vɔ la, woɖonɛ ɖe dodokpɔxɔ aɖe be woadoe kpɔ.

Ne dɔléleŋutinunyala wu flow cytometry dodokpɔa nu ko la, adzro nyatakakaawo me eye wòaɖo nyatakaka ɖe wò ɖɔkta si bia be woawɔ dodokpɔa.

Nu vevitɔe nye be afɔku aɖeke meli si wonya fifia na wò ne èwɔ sisi ƒe lãmenugbagbeviwo ƒe dodokpɔ sia o. Eyata mègavɔ̃ esia madzemadzee o.

Aleke emetsonuwo le? Nukae wogblɔna na mí?

Zi geɖe la, exɔa ŋkeke ʋɛ aɖewo va ɖo kwasiɖa ɖeka hafi wokpɔa sisi ƒe lãmenugbagbeviwo ƒe dodokpɔ me tsonu, le dodokpɔxɔ si wò ɖɔkta zãna nu.

Dɔléleŋutinunyala la léa ŋku ɖe dzesi siwo le wò lãmenugbagbeviwo ƒe anyime, siwo woyɔna be antigens .

• Lãmenugbagbevi si le lãmesẽ me ƒe dɔlékuiwutike ƒe ɖoɖo si sɔ si sɔ na lãmenugbagbevi ƒomevi ma kple eƒe tsitsi ƒe afɔɖeɖe.
• Nɔnɔme sia to vovo le lãmenugbagbevi si mesɔ o me . Tɔtrɔ siawo ate ŋu afia be ʋumenugbagbeviɣidɔ, ʋumenugbagbeviɣidɔ, alo atikewɔwɔ me dɔléle bubuwo le ame ŋu.

Ne wò ɖɔkta xɔ dodokpɔa ŋuti nyatakaka la, ato dɔléleŋutinunyala ƒe nuŋlɔɖia me nyuie, si nye nyatakaka siwo kɔmpiutaa ku tso flow cytometer me ƒe kpukpui.

Ne wò ɖɔkta le wò flow cytometry me tsonu gɔme ɖem la, abu wò dzesiwo, wò lãmesẽ ŋutinya, kple ŋutilãmedɔdada si wowɔ nyitsɔ laa ŋu . Aɖe nu siwo do tso eme la me na wò eye wòaɖe nusi wofia me na wò. Azã nyatakaka siawo hã atsɔ atso nya me le nusi wòawɔ kplɔe ɖo ŋu.

Dɔléle ka ƒomevie nusiwo dona tso eme si mesɔ o ate ŋu afia?

Ne emetsonuwo mesɔ o la, ate ŋu afia nɔnɔmewo abe:

• Ʋumenugbagbeviɣidɔ sesẽ si woyɔna be lymphoblastic leukemia
• Ʋumenugbagbeviɣidɔ sesẽ si woyɔna be myeloid leukemia
• Ʋumenugbagbeviɣidɔ si nɔa anyi didi
• Multiple myeloma ƒe dɔléle
• Non-Hodgkin lymphomas (esiawo ate ŋu anye B-cell kple T-cell ƒomeviwo siaa) .

Ne emetsonuwo mesɔ o la, nukae adzɔ emegbe?

Wò ɖɔkta adzro wò flow cytometry me tsonu kple atikewɔmɔnu siwo nàte ŋui me kpli wò. Dodokpɔ ŋuti nyatakaka sia akpe ɖe wò ɖɔkta ŋu wòase alesi wò dɔlélea nu sẽe, nenye be atike aɖe koŋ sɔ nɛ, kple alesi wòanya wɔ be wòagadzɔ le atikewɔwɔ vɔ megbe gɔme . Azã nyatakaka siawo katã atsɔ awɔ atikewɔwɔ ƒe ɖoɖo si sɔ na wò.

Dɔléle aɖewo xɔa ɣeyiɣi aɖe hafi woate ŋu akpɔ wo. Ðewohĩ èdo dodokpɔ geɖe kpɔ, gake ŋuɖoɖo nyuitɔ megale asiwò kokoko o, eye ɖewohĩ ànɔ ɖokuiwò biam be, "Ke ne mewɔ dodokpɔ bubu ya ɖe?" Gake xɔ dzinye se be flow cytometry dodokpɔ sia ate ŋu ana wò ɖɔkta nakpɔ nyatakaka vevi geɖe tso lãmenugbagbevi siwo le wò ŋutilã me ŋu. Menye ɖeko wòakpe ɖe ŋuwò nànya dɔlélea ko o, ke akpe ɖe ŋuwò hã be nàwɔ atikewɔwɔ ƒe ɖoɖo si sɔ na wò ŋutɔ si sɔ na wò koŋ.

Eyata nu kawo dzie wòle be míaɖo ŋkui tso nusiawo katã me? (Gbedasi si Wotsɔ Yi Aƒeme)

Enyo, mina maƒo nya vevi aɖewo tso nusiwo ŋu míeƒo nu tsoe me kpuie:

• Flow cytometry nye dodokpɔ si de ŋgɔ ŋutɔ si srɔ̃a nu tso lãmenugbagbeviwo ŋu tsitotsito.
• Esia kpena ɖe ame ŋu wòkpɔa ʋumekansa, dɔlélenutsiŋutete ƒe kuxiwo, kple dɔléle bubu geɖe .
• Zi geɖe la, dodokpɔa bia be woaɖe ʋu, ƒumeŋuɖui, alo lãkusi aɖe .
• Dodokpɔ sia mehiã be woadzra ɖo geɖe hafi awɔe o, eye afɔku aɖeke hã mele eme o.
• Nusiwo do tso eme naa ɖɔktaa kpɔa nyatakaka veviwo tso wò nɔnɔmea, atikewɔwɔ si sɔ, kple alesi dɔlélea ayi ŋgɔe ŋu .
• Esia nye dɔwɔnu xɔasi aɖe ŋutɔ si kpena ɖe wò ɖɔkta ŋu wòwɔa atikewɔwɔ ƒe ɖoɖo si sɔ na wò .

Eyata ne ɖɔkta aɖe kafui be woawɔ flow cytometry dodokpɔ na wò alo ame aɖe si nènya la, mègavɔ̃ o. Bu eŋu be enye mɔ si dzi nàto ase wò lãmesẽ gɔme nyuie wu eye wòakpe ɖe ŋuwò nàkpɔ atike nyuitɔ kekeake. Ne nya aɖe le asiwò la, bia wò ɖɔkta.

` Flow cytometry, lãmenugbagbeviwo dodokpɔ, ʋu me kansa, dɔléle didi, dɔlélenutsiŋutete, atikewɔwɔ me dodokpɔwo, lãmenugbagbeviwo me dzodzro