Ðe wodo wò ʋumenugbagbeviɣidɔ kpɔa? Mina míaƒo nu tso Hemoglobin Electrophoresis dodokpɔa ŋu!

Hello! Egbea míele nu ƒom tso ʋudodokpɔ si ate ŋu anye nu yeye vie na wò ŋu, gake ele vevie ŋutɔ le dɔléle aɖewo didi me. Esia nye dodokpɔ si woyɔna be Hemoglobin Electrophoresis . Ðewohĩ wò ɖɔkta bia tso asiwò be nàwɔ dodokpɔ sia, alo èse ŋkɔ sia le afi aɖe. Togbɔ be ŋkɔa didi vie hã la, mègavɔ̃ o! Ele bɔbɔe ŋutɔ. Míaƒo nu tso wo katã ŋu eme nakɔ le nyati sia me egbea.

Nukae nye Hemoglobin Electrophoresis?

Kpuie ko la, esia nye mɔnu si dzi woato adzro protein aɖe si woyɔna be hemoglobin me le míaƒe ʋumenugbagbevi dzĩwo me. Ðewohĩ àɖo ŋku edzi be hemoglobin nye protein vevi aɖe si tsɔa oxygen yia míaƒe ŋutilã katã me eye wònana ʋumenugbagbevi dzĩwo ƒe amadede biã. Míaƒe domenyiŋusẽfianuwoe kpɔa ʋumenugbagbeviɣi sia ƒe wɔwɔ nyuie dzi.

Gake ɣeaɖewoɣi la, tɔtrɔ aɖewo, alo tɔtrɔ aɖewo ate ŋu ava domenyiŋusẽfianu siwo kpɔa ʋumenugbagbeviɣi dzi la me. Ne esia dzɔ la, le ʋumenugbagbevi dzĩ siwo le lãmesẽ me teƒe la, ʋumenugbagbevi dzĩ siwo gblẽ, siwo mewɔa dɔ nyuie o la dzea wɔwɔ gɔme. Lãmenugbagbevi siawo siwo gblẽ la gblẽna kabakaba, si ate ŋu ahe nɔnɔmewo abe ʋusɔgbɔdɔ , alo dɔléle sesẽ siwo do ƒome kple ʋu abe sickle cell anemia kple thalassemia ene vɛ.

Eyata, hemoglobin electrophoresis dodokpɔ sia na ɖɔktawo te ŋu nyaa ne nɔnɔme mawo li. Azɔ hã, dodokpɔ sia nye dodokpɔ ʋɛ siwo wowɔna ɖe vidzĩ yeyewo ŋu la dometɔ ɖeka. Ekpena ɖe ame ŋu be woate ŋu ade dzesi dɔléle siwo mebɔ o gake woate ŋu asẽ ŋu abe sickle cell anemia ene kaba.

Vovototo kae le hemoglobin dodokpɔ kple hemoglobin electrophoresis dome?

Afi sia hãe ame geɖe tɔtɔna le. Hemoglobin dodokpɔ si sɔ dzidzea ʋumenugbagbeviɣi ƒe agbɔsɔsɔ si le wò ʋumenugbagbevi dzĩwo me. Eyae nye be ekpɔnɛ ɖa be wò ʋumenugbagbeviɣidɔ le sue, esɔ gbɔ, alo esɔ hã.

Gake hemoglobin electrophoresis dodokpɔa wɔa nane si de to wu ema sã. Edzroa ʋumenugbagbevi dzĩ ƒomevi vovovo siwo le wò ʋumenugbagbevi dzĩwo me me, kple alesi gbegbe ƒomevi ɖesiaɖe le eme. Èse vovototo si le wo dome gɔmea? Ðeka léa ŋku ɖe agbɔsɔsɔ si le eme ŋu, evelia léa ŋku ɖe ƒomevi mawo ƒe ƒomeviwo kple agbɔsɔsɔme ŋu.

Eyata nukawoe nye hemoglobin ƒomevi siawo?

Zi geɖe la, ɖɔktawo ƒe susu nɔa hemoglobin ƒomevi vevi ene ŋu le dodokpɔ sia me. Na míasrɔ̃ nu tso wo ŋu vie.

• Hemoglobin A1 kple A2 (HgbA1 kple HgbA2): Hemoglobin ƒomevi sia si woyɔna be hemoglobin A1 ye nye esi bɔ ɖe ame tsitsi si le lãmesẽ me ƒe ʋumenugbagbevi dzĩwo me wu. Hemoglobin A2 nye ƒomevi sue aɖe, si ade 2% va ɖo 3% le ʋumenugbagbeviɣi bliboa me. Wogayɔa esiawo be "ame tsitsiwo ƒe ʋumenugbagbeviɣiwo."

• Hemoglobin F (HgbF): Woyɔa esia be "Fetal Hemoglobin".Abe alesi ŋkɔa ɖee fia ene la, HgbF sia ƒomevi le fugboewo, vidzĩ yeyewo, kple ɖevi siwo xɔ abe ƒe 3 ene ƒe ʋumenugbagbevi dzĩwo me. Gake ate ŋu anye nusi mesɔ o be HgbF ƒe agbɔsɔsɔ si sɔ gbɔ sã nanɔ ame tsitsi ƒe ʋu me.

• Hemoglobin S (HgbS): Hemoglobin sia ƒomevi nye ʋusɔgbɔdɔ si me ʋu sina tona le ʋumenugbagbeviɣidɔ me ƒe akpa vevi aɖe . Sickle cell anemia nye dɔléle sesẽ aɖe si le sickle cell dɔlélewo ƒe ƒuƒoƒoa me . Nɔnɔme sia bɔ ɖe amesiwo tso Afrika koŋ me . HgbS nana ʋumenugbagbevi dzĩwo trɔna zua sickle ƒe nɔnɔme, si ate ŋu axe ʋukawo.

• Hemoglobin C (HgbC): Hemoglobin sia ƒomevi do ƒome kple nɔnɔme aɖe si woyɔna be hemolytic anemia . Esia nye nɔnɔme si me ʋumenugbagbevi dzĩwo tsrɔ̃na kabakaba wu lãmenugbagbevi siwo sɔ alo woƒe agbenɔƒe nɔa kpuie. Abe ʋusɔgbɔdɔ si woyɔna be sickle cell anemia ene la, HgbC ƒe nɔnɔme sia hã bɔ ɖe amesiwo tso Afrika me wu .

Fifia edze ƒã na wò be hemoglobin ƒomevi vovovowoe li, eye dɔwɔwɔ kple ŋusẽkpɔɖeamedzi tɔxɛ aɖewo le ƒomevi ɖesiaɖe si.

Aleke wowɔa dodokpɔ siae? Aleke woɖea ʋuae?

Be míate ŋu awɔ dodokpɔ sia la, ʋu ƒe dodokpɔ koe míehiã. Ðɔktawo, mɔ vovovowo li siwo dzi woato axɔ ʋu sia. Na míakpɔ nusiwo wonye ɖa.

1. Ʋu si woɖena le ʋukawo me

Esiae nye mɔnu si bɔ tsɔ xɔa ʋu tso ame tsitsiwo kple ɖevi tsitsiwo gbɔ. Ðewohĩ èna ʋu to mɔ sia dzi.

• Gbã la, dɔnɔdzikpɔla alo ɖɔkta atsɔ aha akɔ teƒe si wò ʋuka le wò alɔnu.
• Emegbe, wotsɔa tourniquet dea wò alɔnu heblanɛ sesĩe, eye wobiana tso asiwò be nàmimi wò alɔnu. Esia ana be ʋukawo kpɔkpɔ nanɔ bɔbɔe eye ʋua hã naɖe bɔbɔe.
• Eyome, dɔnɔdzikpɔla la tsɔa aŋe dea ʋukaa me. Le ɣeyiɣi sia me la, àte ŋu ase veve vie, abe anyidi ɖu wò ene. Gake sɛkɛnd ʋɛ aɖewo koe wòxɔna.
• Ƒo ʋu agbɔsɔsɔme si hiã la nu ƒu ɖe go sue aɖe me eye nàɖoe ɖe dodokpɔxɔa be woadoe kpɔ.
• Ne woɖe ʋua vɔ la, wotsɔa aŋɔ sue aɖe dea teƒe si wotsɔ aŋea de. Teƒea ate ŋu aƒo vie eye ate ŋu azu blɔ ŋkeke ʋɛ aɖewo gɔ̃ hã. Esia sɔ, mègatsi dzi o.

2. Asibidɛ ƒe atikplɔ

Zi geɖe la, wozãa mɔnu sia tsɔ ɖea ʋu le ɖevi suewo ŋu.

• Esia bia be woatsɔ dɔwɔnu sue aɖe aƒo ɖevia ƒe asibidɛ nu eye woaɖe ʋu ʋɛ aɖewo.
• Wotsɔa ʋua dea dodokpɔmɔ̃ si ŋu wotrɔ asi le etɔxɛe dzi alo dea aŋetu sue aɖe me.
• Ɣeaɖewoɣi la, mɔ̃ aɖewo li siwo ate ŋu aƒo ʋu nu ƒu ahade eme le ɣeyiɣi ɖeka me. Esia nye mɔnu si zɔna kabakaba ŋutɔ eye wòle bɔbɔe.

3. Afɔkpodzi ƒe ati

Wowɔa esia abe vidzĩ yeyewo ƒe lãmesẽ dodokpɔ ƒe akpa aɖe ene. Zi geɖe la, wowɔa dodokpɔ sia le gaƒoƒo 48 me tso esime wodzi vidzĩa.

• Le esia me la, wotsɔa aŋe sue aɖe dea vidzĩa ƒe afɔkpodzi ƒe akpa si le ete eye woɖea ʋu ʋɛ aɖewo.
• Le ɣeyiɣi sia me la, vidadaa alo fofoa ate ŋu alé vidzĩa. Ekema vidzĩa hã ase le eɖokui me be yefa akɔ na ye.
• Woxɔa ʋua heɖonɛ ɖe dodokpɔxɔa me ne wotsɔe sisi ɖe pepa aɖe dzi vɔ.
• Wozãa dodokpɔ siawo tsɔ doa vidzĩ yeyewo kpɔna be nɔnɔme siwo mebɔ o gake woate ŋu ada wo abe ʋusɔgbɔdɔ si woyɔna be sickle cell anemia ene ƒe dzesiwo le wo ŋu hã, si fia be ʋumenugbagbeviɣi ƒe vovototo siwo mesɔ o le wo ŋu.

Aleke woada ʋu sia kpɔ le dodokpɔxɔ me?

Enyo, fifia míexɔ ʋua. Nukae dzɔna emegbe? Le dodokpɔxɔa me la, woɖea ʋumenugbagbevi dzĩ ƒomevi siawo ɖe vovo hedea dzesi wo le mɔ si doa dzidzɔ na ame ŋutɔ nu. Woyɔa ema be elektrikŋusẽ zazã . Elektrikŋusẽ vovovowoe le hemoglobin ƒomevi veviawo ŋu.

Alesi dodokpɔ sia wɔa dɔ zi geɖee nye esi:

• Gbã la, woɖea ʋumenugbagbevi dzĩawo ɖa le ʋua me eye woɖea wo ɖa. Wotsɔa ʋumenugbagbevi dzĩ sia si wotsɔ ƒo ƒui la dana ɖe lãgbalẽ tɔxɛ aɖe abe cellulose-ti ene dzi .
• Emegbe wotsɔa ka si me kpɔɖeŋua le la dana ɖe mɔ̃ aɖe si woyɔna be electrophoresis chamber . Mɔ̃ sia toa elektrikŋusẽ aɖe to kpɔɖeŋua me.
• Le elektrikŋusẽa ta la, hemoglobin ƒomevi siwo ƒe elektrikŋusẽ to vovo la ɖea wo nɔewo ɖa eye wozɔna le ʋuƒoa dzi le duƒuƒu vovovowo me.
• Mlɔeba la, hemoglobin ƒomevi siawo siwo mã la dzena abe amadede vovovowo ƒe ka ene le lãgbalẽgoloa dzi.
• Eyome atikewɔlawo tsɔa dodokpɔ siawo me tsonu sɔna kple ʋumenugbagbeviɣidɔ si sɔ si wokpɔ tso ame si le lãmesẽ me gbɔ.
• Ne ʋumenugbagbeviɣidɔ ƒomevi aɖe ƒe agbɔsɔsɔ sɔ gbɔ sã alo ɖiɖi sã wu alesi wòle be wòanɔ la, ate ŋu anye ʋumedɔléle aɖe ƒe dzesi. Le kpɔɖeŋu me, ne wò hemoglobin S (HgbS) ƒomevi ƒe dzedzeme to vovo na ame dzɔdzɔe ƒe HgbS la, ate ŋu anye dzesi be ʋusɔgbɔdɔ le sickle cell le ŋuwò.

Bu eŋu ale, abe alesi ne ètsɔ kpe gbadzɛ siwo ƒe kpekpeme to vovo ƒu gbe ɖe tsi ƒe tɔsisi aɖe me ene la, kpe gbadzɛawo zɔa mɔ vovovowo le woƒe kpekpeme nu. Le afisia la, elektrikŋusẽ wɔa dɔ abe "tsi ƒe tɔsisi" ma ene.

Vovototo kae le isoelectric focusing (IEF) kple electrophoresis dome?

Isoelectric focusing (IEF) nye mɔnu bubu si wozãna tsɔ dea dzesi ʋumenugbagbeviɣi ƒomevi siwo mesɔ o. IEF kple electrophoresis siaa zãa elektrikŋusẽ tsɔ ma hemoglobin ƒomeviwo dome. Gake mɔ̃ɖaŋununya ƒe vovototo aɖewo le mɔnu eveawo dome. Ɣeaɖewoɣi la, woate ŋu azã mɔnu evea siaa atsɔ ade dzesi ʋumenugbagbeviɣi ƒomeviwo nyuie wu.

Ɣeyiɣi didi kae wòxɔna hafi akɔntabubua va ɖoa?

Dodokpɔa ate ŋu axɔ abe gaƒoƒo ɖeka ene hafi woawu enu le dodokpɔxɔa me. Gake ate ŋu axɔ ŋkeke ʋɛ aɖewo hafi wò ɖɔkta naxɔ dodokpɔa me tsonu, alé ŋku ɖe wo ŋu, eye wòaɖe wo me na wò. Eyata mègakpɔ mɔ be nyatakakaa nava enumake le dodokpɔa megbe o. Ðɔktaa agblɔ gbesigbe wòahiã be nàva xɔ nyatakakaa na wò.

Nukae nye nyatakakaa fia? Aleke mase egɔmee?

Esiae nye nu vevitɔ kekeake. Wò ʋumenugbagbeviɣidɔ ŋuti nyatakakaa ayɔ ʋumenugbagbeviɣidɔ ƒomevi ɖesiaɖe ƒe didime aɖewo. Le kpɔɖeŋu me, ne wò hemoglobin F (HgbF) ƒe agbɔsɔsɔ sɔ gbɔ sã wu alesi wòle dzɔdzɔmee la, ate ŋu anye thalassemia ƒomevi aɖe ƒe dzesi.

Gake, ele be nàɖo ŋku esia dzi nyuie:

Mèganɔ te ɖe xexlẽme siwo le nyatakakaa me dzi awɔ nyametsotso le dɔléle aɖeke ŋu o. Ema nye dɔlélea ƒe akpa ɖeka ko. Ame nyuitɔ si ase nusi tututu le wò nyatakakaa me kple alesi wòkpɔa ŋusẽ ɖe wò lãmesẽ dzii gɔmee nye wò ɖɔkta . Abu nusianu ŋu tso wò dzesiwo, dodokpɔ bubuwo me tsonu, kple wò atikewɔwɔ ŋutinya dzi be yeakpɔ dɔlélea nyuie.

Ne nu siwo mesɔ o le nye nyatakakaa me la, ɖe wòle be maƒo ka na ɖɔktaa?

Ẽ, keŋkeŋ. Ne wowɔ hemoglobin electrophoresis dodokpɔ sia le dzesi aɖewo ta (e.g., ɖeɖiteameŋu enuenu, ɣie, gbɔgbɔtsixetsixe) la, ele be nàkpɔ wò ɖɔkta enumake adzro nyatakakaa me tsonu me.

Hemoglobin electrophoresis dodokpɔ nana míekpɔa hemoglobin ƒomevi siwo le wò ʋu me le ɣeyiɣi ma me ƒe nɔnɔmetata. Hemoglobin ƒe agbɔsɔsɔ nye mɔ si dzi míato akpɔ protein vevi sia le míaƒe ʋumenugbagbevi dzĩwo me. Tɔtrɔ si mesɔ o le ʋumenugbagbevi dzĩ ƒomevi aɖe ƒe agbɔsɔsɔ me ate ŋu anye dzesi be ɖewohĩ atikewɔwɔ me dɔléle aɖe le ŋuwò.

Ne viwòa alo viwò ƒe dodokpɔ me tsonu ɖee fia be dɔléle sesẽ aɖe le eŋu la, ele bɔbɔe be nu natsi dzi na wò eye vɔvɔ̃ naɖo ŋuwò ne èlé ŋku ɖe nɔnɔmetata la ŋu ko. Vevietɔ ne viwò sue alo viwò yeye ƒe lãmesẽ ŋue wòku ɖo la, esɔ be nàse le ɖokuiwò me be yegbɔdzɔ. Gake dze agbagba nàlé ŋku ɖe nu gã la ŋu le esi teƒe be nàtsi dzodzodzoe. Ame nyuitɔ si akpe ɖe ŋuwò le ema ŋue nye wò ɖɔkta. Ate ŋu aɖe alesi "nɔnɔmetata" sia sɔ ɖe wò lãmesẽ bliboa nu kple nusi wòle be nàwɔ emegbe la me.

Gbedasi si Wotsɔ Yi Aƒeme

Mele mɔ kpɔm be èse hemoglobin electrophoresis dodokpɔ si ŋu míeƒo nu tsoe egbea gɔme nyuie azɔ. Nu ʋɛ aɖewo siwo wòle be wòanɔ susu me na wòe nye esi:

• Hemoglobin electrophoresis nye ʋudodokpɔ tɔxɛ aɖe si me woɖea ʋumenugbagbeviɣidɔ ƒomevi vovovo siwo le ʋua me kple woƒe agbɔsɔsɔ fiana.
• Esia kpena ɖe ame ŋu wòkpɔa dɔléle siwo do ƒome kple ʋu abe sickle cell anemia kple thalassemia ene .
• Vidzĩ si wodzi yeyee ƒeWowɔa esia hã abe lãmesẽ dodokpɔ ƒe akpa aɖe ene.
• Woɖea ʋumenugbagbevi dzĩ ƒomeviwo ɖe vovo to ʋu ɖeɖe kple elektrikŋusẽ zazã me .
• Ƒo nu kple wò ɖɔkta ɣesiaɣi hafi nàdze agbagba ase nyatakakaa me tsonu gɔme le ɖokuiwò si.
• Ne emetsonuwo mesɔ o la, mègatsi dzodzodzoe o eye nàwɔ ɖe wò ɖɔkta ƒe mɔfiamewo dzi. De dzesii kabae nye afɔɖeɖe gbãtɔ si ana woada dɔa dzidzedzetɔe.

Míele mɔ kpɔm be nyatakaka sia aɖe vi na wò. Nɔ lãmesẽ me!

` Hemoglobin Electrophoresis, Hemoglobin, Ʋumenugbagbevi dzĩwo, Ʋusɔgbɔdɔ, Sickle Cell Ʋusɔgbɔdɔ, Thalassemia