Wetin na Immunostaining? Lɛ wi tɔk bɔt dis tɛst fɔ no if pɔsin gɛt sik insay simpul wɔd dɛn!

yu don eva geht dokta oda wan tehst we dem kol "Immunostaining"? Ɔ yu dɔn yɛri di wɔd sɔmsay? Sɔntɛm i kin tan lɛk se i kɔmplikt smɔl to yu. Bɔt nɔ wɔri, tide wi go tɔk bɔt dis tɛst we dɛn kɔl Immunostaining, wetin i de du, aw dɛn de du am, ɔltin na wan rili simpul we we yu go ɔndastand. Jɔs lɛk we yu de tɔk to yu padi.

Wetin rili na immunostaining?

Fɔ tɔk am simpul wan, immunostaining na spɛshal tɛst we wi pathɔlɔjis dɛn kin yuz. Dɛn kin yuz am fɔ no wan patikyula prɔtin mɔlyul bitwin bɔku prɔtin dɛn we de na sɛmpul we dɛn tek frɔm yu bɔdi, lɛk blɔd, bon mɛro, ɔ tisu. Tink bɔt am lɛk we yu fɛn wan patikyula pɔsin insay krawd. Dis we fɔ du tin kin rili ɛp fɔ no difrɛn sik dɛn, mɔ di wan dɛn we gɛt infɛkshɔn ɛn kansa.

Aw dis tɛst de wok?

Okay, naw mek wi si aw dis immunostaining de wok. I de yuz sɔntin we dɛn kɔl antibodies . Dɛn antibodi ya tan lɛk sojaman dɛn na wi bɔdi in difens sistɛm. Dɛn kin no ɛn atak tin dɛn we kin mek pɔsin gɛt sik, we dɛn kɔl antijen . So, insay dis tεst, di dכkta dεm de yuz wan antibodi we spεshal fכ da protin (dεn kכl am antijen) we dεn de luk fכ.

Dɔn di "staining" pat de kam. I tan lɛk se yu de aylayt wan impɔtant sɛntɛns na buk. Bɔt i nɔ simpul lɛk fɔ kɔlɔ T-shirt. dis "staining" mεtכd dεm de yuz spεshal day, εnzym dεm we de chenj kכla, fכrosεnt mכlikul dεm, εn ivin gold patikyula dεm. dis dεm de lεbul di protin dεm εn sεl dεm we dεn de luk fכ, so di wan dεm we de du risach kin si dεm klia wan כnda maykroskכp.

Bifo yu du dis immunostaining test, dɔktɔ go tek yu blɔd, bon mɛro, ɔ tisu. Dɔn dɛn go sɛn am to dɔktɔ we de mɛn sik dɛn fɔ mek dɛn tɛst am. Mɛmba se, ilɛksɛf dɛn tek di sampul na ɔspitul ɔ na di dɔktɔ in ɔfis, dɛn kin du dis immunostaining tɛst ɔltɛm na lɛbɔtri, ɔ wetin wi kɔl lab.

Wetin na di kayn immunostaining?

Bɔku we dɛn de fɔ mek di bɔdi gɛt di sik we dɛn kɔl immunostaining we dɔktɔ dɛn kin yuz. Ɛni we fɔ du am gɛt wan patikyula we fɔ tɛst, ɔ protɔkɔl, we gɛt fɔ du wit am. Na sɔm pan di men we dɛn we dɛn kin yuz:

• Immunohistochemistry we dɛn kin du
• Flɔ saytometri fɔ yuz
• Imyuno-ilɛktrɔn maykroskɔpi (EM immunolabeling) .

Wi go luk ɛni wan pan dɛn tin ya smɔl mɔ?

Immunohistochemistry - di mכst we dεn kin yus

Dis na di mכst kכmכn we dεn kin yus fכ immunostaining mεtכd . insay imyunohistochemistry, dεn kin put sכm sכm pat pan di sεmpl pan glas slayd, εn dεn ad wan antibodi we de tay pan di tכgεt to am. Dɔn dɛn kin stɛyn am wit spɛshal day ɔ ɛnzaym dɛn. Dɛn kɔl dɛn tin ya immunostains. dis kin mek di patכlayzm si sכm protin dεm εn sεl dεm rili klia wan כnda maykroskכp.

Si wetin na di bɛnifit dɛn we immunohistochemistry gɛt:

• I de ɛp fɔ no difrɛn kayn kansa.
• I kin ɛp fɔ klas bɔku bɔku nyurolɔjik kɔndishɔn ɛn blɔd dizayd.
• Dis kin mek dɔktɔ dɛn ebul bak fɔ stɔdi bɔt aw sɔm jin dɛn kin afɛkt, ɔ aw dɛn kin sho am .

fכ fכlכ saytכmetri - lεk mashin we de kכnt εn separet sεl dεm

Insay wan we we dɛn kɔl flɔ saytometri, wan tɛknishian kin yuz antibodi dɛn we gɛt kɔlɔ lɛbl fɔ mak di prɔtin dɛn we dɛn de luk fɔ. Dɔn, dɛn kin yuz wan spɛshal lɛsa bim fɔ kɔnt, sɔt, ɛn separet yu sɛl dɛn wan bay wan. I tan lɛk se yu de skan tin dɛn we yu de bay na supamakit.

Dɔktɔ dɛn kin yuz flɔ saytometri fɔ no sik dɛn lɛk:

• Sik dɛn we pɔsin kin gɛt
• Di sik dɛn we pɔsin kin gɛt we i de fɛt insɛf
• Lukimiya
• Limfoma we dɛn kɔl Limfoma

Imyuno-ilɛktrɔn maykroskɔpi (EM immunolabeling) .

Imyuno-εlektrכn maykroskכpi de alaw di patכlayz fכ si yu sεmpl wit bכku bכku magnifyush כn εn in bכku fayn ditel. insay dis, di patכlayz dεm de ataya sכm sכm gold patikyula dεm we dεn kכl kolכyd gold patikyula dεm to antibodi dεm. Dis kolכyd gold rili izi fכ si כnda εlektrכn maykroskכp. So, we da antibodi de go grap di antijen, di gold patikyula dεm de mek i klia klia wan lεk blak dכt dεm.

Apat frɔm we dɛn kin no difrɛn vayral infɛkshɔn ɛn sik dɛn, imyuno-ɛlektrɔn maykroskɔpi kinIvin fɛn di vayral antijen dɛn na di vaysin dɛn.

Aw a fɔ rɛdi fɔ dis tɛst?

Bɔrku tɛm, yu nɔr nid fɔ pripia fɔ wan immunostaining test. Bɔt yu dɔktɔ go gi yu instrɔkshɔn bifo i tek yu blɔd, tisu, ɔ bon mɛro sɛmpul. Dis go ɛp yu fɔ no gud gud wan wetin yu fɔ ɛkspɛkt. I impɔtant fɔ tek tɛm lisin to wetin yu dɔktɔ se.

Aw lɔng di tɛst kin tek? Aw lɔng i kin tek fɔ mek di rizɔlt kam bak?

Dis rili dipen pan di immunostaining we yu dɔktɔ de yuz. Sɔm we dɛn kin yuz fɔ du tin kin tek sɔm awa nɔmɔ , ɛn ɔda wan dɛn kin tek 10 dez . Yu dɔktɔ go ebul fɔ tɛl yu aw lɔng i go tek bay aw yu de si yu.

Di tɛm we i kin tek fɔ mek di rizɔlt kam bak na di sem tin bak. Sɔm imyunostayning we dɛn kin yuz kin gi yu rizɔlt insay smɔl lɛk wan de. Ɔda wan dɛn kin tek wan wik ɔ pas dat. We yu gi di sampul, yu dɔktɔ go mek yu no ustɛm yu go ebul fɔ ɛkspɛkt di rizɔlt.

So, na tɛm lɛk dis, i bɛtɛ fɔ peshɛnt ɛn nɔ panik. Tɔk to di dɔktɔ ɛn mek ɔltin klia.

Wetin di rizɔlt dɛn se? Wetin kin apin if dɛn nɔrmal?

Di pathɔlɔjis go analayz yu tɛst rizɔlt ɛn sɛn am to yu dɔktɔ. Di rizulyt wae yu kin gɛt kin difrɛn difrɛn wan bay di kayn tɛst wae di pathɔlɔjis du. Yu dɔktɔ go ɛksplen yu tɛst rizɔlt ditayli ɛn wetin dɛn min fɔ yu patikyula sityueshɔn.

Mɛmba se, if di tɛst rizɔlt nɔrmal , we min se di dɔktɔ we de mɛn di sik fɛn sayn dɛm fɔ wan sik, nɔ panik. Yu dɔktɔ go ɛksplen to yu bɔt di tin dɛn we go apin to yu ɛn tɔk to yu bɔt aw fɔ trit yu. Sɔntɛnde, wan tim fɔ dɔktɔ dɛn kin wok togɛda fɔ mek di bɛst tritmɛnt plan.

Fɔ dɔn, di tin dɛn we impɔtant pas ɔl we wi fɔ mɛmba

Okay, so frɔm wetin wi dɔn tɔk bɔt, a op se yu dɔn gɛt gud aidia bɔt immunostaining. Na sɔm impɔtant tin dɛn we yu fɔ mɛmba:

• Immunostaining na labכtכri tεst we de yuz antibodi dεm fכ no εn lεbul spεsifi k antijen dεm insay wi sεl dεm.
• Dis tɛst kin ɛp fɔ no difrɛn kayn wɛl bɔdi prɔblɛm, lɛk vayral ɛn baktriyal infɛkshɔn, ɛn sɔm kayn kansa.
• If yu fɔ du immunostaining test, no rizin nɔ de fɔ mek yu fred.Yu dɔktɔ go ɛksplen am to yu ditayli.
• Wae de test rizulyt dɔn, tɔk opin wan wit yu dɔktɔ bɔt wetin dɛn min fɔ yu sik ɛn if yu nid tritmɛnt ɔr nɔr nid.

Mɛmba se, mɛrɛsin sayɛns de chenj ɔltɛm. Na tru tɛst lɛk dis, wi kin no bɔku sik dɛn kwik kwik wan ɛn trit dɛn fayn fayn wan. So, if dɔktɔ tɛl yu fɔ du dis kayn tɛst, i impɔtant fɔ mek yu rɛdi fɔ am.

` Immunostaining, Patɔlɔji, Antibodi, Antijen, Kansa Tɛst, Labɔrɔtɔri Tɛst