An ka PCR sɛgɛsɛgɛli dɔn nɔgɔya la, i n’a fɔ an terikɛ dɔ fɛ cogo min na!

Aw hakili bɛ se ka to a la ko Covid-19 banakisɛ waati la, an bɛɛ kumana PCR sɛgɛsɛgɛli kan . N’a sɔrɔ mɔgɔ dɔw siranna dɔɔnin, ka miiri bere dɔ la min donna nugu la wa? Nka nin PCR sɛgɛsɛgɛli in ye mun ye tigitigi? Yala a bɛ se ka Covid-19 dɔrɔn de dɔn wa? Ayi, PCR ye sɛgɛsɛgɛli fɛɛrɛ ye min nafa ka bon kosɛbɛ furakɛli la min bɛ dɛmɛ don fɛn caman na. An ka kuma nin bɛɛ kan bi, cogo nɔgɔman na ani cogo jɛlen na, i n’a fɔ an bɛ kuma an teri ɲuman dɔ fɛ.

PCR ye mun ye? Yala i bɛ se k’o ɲɛfɔ dɔɔnin wa?

N’an y’a fɔ cogo nɔgɔman na, PCR ye Polymerase Chain Reaction (Polymerase Chain Reaction) daɲɛ surun ye. A bɛ kɛ ka jamu fɛnw dɔn an farikolo la, ADN walima ARN . Miiri k’a filɛ, fɛn fitinin dɔ bɔra aw la, a bɛ se ka kɛ joli dɔ ye, walima ni sɔgɔsɔgɔninjɛ ye ka bɔ aw nu kɔnɔ. O ADN walima ARN fitinin min bɛ o sɛgɛsɛgɛli la, o bɛ ta ka miliyɔn caman, n’a sɔrɔ miliyari caman, olu bɛ dilan laboratuwari kɔnɔ. O de ye dɔnnikɛlaw bɛ min Weele ko amplification , o kɔrɔ ye ko "ka caya" walima "ka caya". A bɛ iko ka papiye kelen kopi caman dilan ni fotokopikɛlan ye.

Ni u ye kopi caman dilan i n’a fɔ nin, a bɛ nɔgɔya dɔnnikɛlaw ma ka ADN walima ARN sɛgɛsɛgɛ o fɛn fitinin fɔlɔ kɔnɔ, k’a lajɛ ni fɛn kɛrɛnkɛrɛnnen dɔ bɛ a kɔnɔ. PCR bɛ sɛgɛsɛgɛli kulu dɔ kɔnɔ min bɛ wele ko Nucleic Acid Amplification Tests (NAAT) .

Hali ni mɔgɔ caman ye PCR daɲɛ mɛn ka ɲɛsin Covid-19 sɛgɛsɛgɛli ma, o fɛɛrɛ in kɛra furakɛli siratigɛ la kabini waati jan, wa a ye fɛɛrɛ ye min nafa ka bon kosɛbɛ.

Mun bɛ se ka dɔn ni PCR ye?

Sisan an k’a lajɛ nin PCR sɛgɛsɛgɛli in bɛ se ka fɛn sugu minnu dɔn. Mɔgɔ caman hakili la ko Covid-19 dɔrɔn de don. Nka o tɛ ten. Aw ye a lajɛ:

• Banakisɛfagalanw : PCR bɛ se ka dɛmɛ don ka a dɔn ni sumaya, farigan walima sɔgɔsɔgɔ min bɛ aw la, o sababu bɔra banakisɛ , banakisɛ , ɲɛgɛn , walima paraziti fɛ . I n'a fo:
• SARS-CoV-2 banakisɛ min bɛ COVID-19 (COVID-19) lase mɔgɔ ma .
• Grippe , an ka teli ka o de wele ko mura.
• Ninakilibanakisɛ (RSV), o min ka ca kɛrɛnkɛrɛnnenya la den fitiniw na.
• Banakisɛ min bɛ sumaya bila mɔgɔ la.
• Banakisɛ ni banakisɛ caman wɛrɛw.
• Bana minnu bɛ sɔrɔ jamu fɛ: Bana minnu bɛ bɔ mɔgɔ dɔw la, olu bɛ sɔrɔ ciyɛn fɛ, tiɲɛ tɛ? O bana suguw, misali la , Fibrose Cystique.Nin PCR in bɛ kɛ fana ka bana dɔw sɛgɛsɛgɛ i n’a fɔ Huntington ka bana ani Fragile X Syndrome . Ninnu ye banaw ye minnu ka gɛlɛn kosɛbɛ, o la, u dɔnni joona nafa ka bon kosɛbɛ furakɛli la.
• Kansɛri suguyaw : Nin fɛɛrɛ in bɛ dɛmɛ fana ka kansɛri suguya dɔw dɔn, i n’a fɔ kansɛri .
• Jɛnɛya caman yeli kansɛri la: Ni kansɛri bɛ mɔgɔ min na, ni o tigi furakɛra, nin PCR sɛgɛsɛgɛli in bɛ se ka jɛnɛya caman ɲɔgɔnna/fɛnɲɛnamafagalan kɛrɛnkɛrɛnnenw fana dɔn o kansɛribanakisɛw la. O bɛna a to furakɛli laɲinitaw ka se ka kɛ walasa ka o fɛn kɛrɛnkɛrɛnnenw caman wuli. O ye ɲɛtaa ba ye kansɛri furakɛli siratigɛ la.
• Bana nataw farati : Mɔgɔ minnu ka jamu caman bɛ ɲɔgɔn na, olu bɛ se ka bana juguw sɔrɔ i n’a fɔ kansɛri don nataw la. nin PCR in bɛ kɛ fana ka jamu sɛgɛsɛgɛli kɛ kansɛri farati kan . O b’a to aw bɛ kɔn ka o dɔn ani ka ɲɛnamaya kɛcogo Changer ni a mago bɛ a la, walima ka to aw ɲɛ na ni aw bɛ to ka sɛgɛsɛgɛli kɛ.
• Sɛgɛsɛgɛli minnu bɛ kɛ ka kɔn jiginni ɲɛ : PCR fana bɛ dɛmɛ don sɛgɛsɛgɛliw la minnu bɛ kɛ walasa ka a dɔn ni musokɔnɔma den min tɛ kɔnɔbara la, ni jamu bana dɔ bɛ a la.

O dɔrɔn tɛ, n’a sɔrɔ i y’a ye filimuw kɔnɔ, ADN daliluw, i n’a fɔ kunsigi fitinin walima joli nɔgɔ min sɔrɔla kojugukɛyɔrɔ la, olu bɛ ta ka kɛ ka kojugukɛla dɔn. Forensic laboratuwariw fana bɛ baara kɛ ni nin PCR fɛɛrɛ in ye walasa ka dɔ fara ADN hakɛ kan ani ka sɛgɛsɛgɛliw kɛ.

Sɛgɛsɛgɛliw ni fɛɛrɛ jumɛnw bɛ baara kɛ ni PCR fɛɛrɛ ye?

PCR fɛɛrɛ bɛ kɛ sɛgɛsɛgɛli fɛɛrɛ suguya caman na. Aw ka kan ka sɛgɛsɛgɛli suguya min kɛ, o bɛna bɔ aw bɛ sɛgɛsɛgɛli kɛ min na. Misali dɔw filɛ nin ye:

• Joli sɛgɛsɛgɛli : Joli bɛ ta ka PCR kɛ ka jamu caman yeli ni banakisɛ dɔw dɔn.
• Nasofarinzi : Nɔgɔ min bɛ bɔ nugu kɔnɔ walima nugu kɔnɔ, o bɛ se ka kɛ ka ninakilibanaw sɛgɛsɛgɛ i n’a fɔ kunfilanitu (mura), COVID-19, ani RSV. Nin ye an bɛɛ ye COVID waati la.
• Preinplantation Genetic Diagnosis (PGD) walima Preimplantation Genetic Screening (PGS): Denso kɔnɔbara basigilen (IVF) kɛcogo la, denso kɔnɔfɛn damadɔw bɛ ta ka u sɛgɛsɛgɛ ni u ka jamu cogoyaw tɛ sani u ka don ba denso kɔnɔ. A ka ca a la o bɛ kɛ tile damadɔ kɔnɔ kɔnɔbara bɔlen kɔfɛ.
• Amniocentesis (banakisɛfagalanw) :Kɔnɔmaya waati, kɔnɔbara basigilen dɔɔni min bɛ den lamini ba kɔnɔbara la, o bɛ se ka kɛ ka den ka jamu cogoya sɛgɛsɛgɛ ni PCR ye. Nin ye sɛgɛsɛgɛli ye min bɛ kɛ dɔgɔtɔrɔ kɛrɛnkɛrɛnnen dɔ fɛ.
• Biopsie : Walasa ka jamu caman sɛgɛsɛgɛ kansɛribanakisɛw la (sɛgɛsɛgɛli molekiyɔmu), tumu fitinin dɔ bɛ ta (tumor biopsie) walima kolotugudaw biyopisi. O bɛ i n’a fɔ opereli fitinin dɔ. O kɔfɛ, o sɛgɛsɛgɛli bɛ kɛ ka PCR kɛ walasa ka jamu caman yeli ɲini.

I bɛ i labɛn cogo di o kɔrɔbɔli kama?

A ka ca a la, labɛn kɛrɛnkɛrɛnnen si man kan ka kɛ PCR sɛgɛsɛgɛli kama. Nka, a bɛ bɔ aw bɛ fɛn min sɛgɛsɛgɛ ani fɛɛrɛ min bɛ kɛ ka sɛgɛsɛgɛli kɛ. Misali la, joli sɛgɛsɛgɛli dɔw bɛ se ka kɛ sababu ye ka sunɔgɔ lɛri damadɔw kɔnɔ ka kɔn o ɲɛ. O la, a ka fisa aw ka dɔgɔtɔrɔ walima sɛgɛsɛgɛlikɛyɔrɔ ɲininka ni fɛn kɛrɛnkɛrɛnnen dɔ bɛ yen aw ka kan ka min kɛ.

PCR bɛ baara kɛ cogo di? Dɔnniya dɔɔnin!

Nin ye dɔnniya maana ye dɔɔnin, nka a to n k’a ɲɛfɔ nɔgɔya la. PCR bɛ min kɛ, o ye ka ɲɔgɔndan dɔ dɔn min ɲɔgɔn tɛ ADN la i b’a fɛ ka min ɲini, o kɔrɔ ye ko a kɛrɛnkɛrɛnnen don o la. O ɲɔgɔndan bɛ se ka kɛ kansɛri dɔ ka jamu caman yeli ye, walima a bɛ se ka kɛ banakisɛ walima banakisɛ dɔ ka jamu kode ye. o bɛ wele ko ADN laɲinilen .

Tɔgɔ min ye ko "Polymerase Chain Reaction" o bɛ tali kɛ fɛɛrɛ la min bɛ kɛ ka ADN laɲinilen dɔ kopi caman dilan (ka bonya) laboratuwari kɔnɔ. o kɛcogo in bɛ kɛ ni anzimu dɔ ye min bɛ wele ko ADN polimerazi . O anzimu bɛ ADN laɲinilen kopiw dilan, ni a bɛ sɔrɔ sɛgɛsɛgɛli la.

O "cakɛda" bɛ Kɛ ni furaji dɔ bɛ Tɛmɛ funteni ni nɛnɛcogo caman fɛ. Siginiden kelen kɔnɔ, ADN hakɛ bɛ se ka kɛ siɲɛ fila ye. Miiri k’a filɛ i n’a fɔ kelen ka se fila ma, fila ka se naani ma, naani fo seegin... ani o ɲɔgɔnnaw, ka laban ka kɛ miliyɔn caman ye. O nafa ye ko PCR bɛ se ka ADN walima ARN hakɛ fitininw yɛrɛ sɔrɔ, n’o tɛ, sɛgɛsɛgɛli wɛrɛw tun bɛna to u kɔ.

Tuma caman na, PCR kɛlen kɔfɛ, dɔnnikɛlaw ka kan ka sɛgɛsɛgɛli caman kɛ walasa ka ADN camanba sɛgɛsɛgɛ. Nka tuma dɔw la, taamasiɲɛ kɛrɛnkɛrɛnnenw bɛ fara furaji kan PCR kɛcogo la, o bɛ se k’a dɔn ni sɛgɛsɛgɛli kɛra a la walima ni a man ɲi. Nin fɛɛrɛ in bɛ se ka kɛ walasa ka nɔ sɔrɔ joona, kɛrɛnkɛrɛnnenya la ni aw bɛ banakisɛw ɲini i n’a fɔ banakisɛw.

PCR suguya jumɛnw bɛ yen?

PCR suguya wɛrɛw bɛ yen. Dɔw bɛ fɛɛrɛ wɛrɛw wajibiya walasa ka ADN kopi kɛ walima k’a sɛgɛsɛgɛ. An ka suguba fila lajɛ:

1. Ɲɛgɛnɛsiraw labɛncogo (RT-PCR) .

Miiri k’a filɛ, an b’a fɛ ka kunnafoni jamu minnu kopi kɛ ani k’u ɲini, olu tɛ ADN kɔnɔ, nka u bɛ ARN kɔnɔ. (Misali la, Covid-19 banakisɛ bɛ ni ARN ye). O kɔfɛ, nin RT-PCR , min bɛ kɛ, o ye ko ARN (ARN) bɛ fɔlɔ ka wuli ka kɛ ADN ye ni anzimu kɛrɛnkɛrɛnnen dɔ ye (reverse transcriptase). ADN min bɛ dilan, o bɛ wele ko ADN dafalen (ADNc) . O kɔfɛ, PCR kɛcogo nɔgɔman min kofɔra ka tɛmɛ, o bɛ kɛ. Sabula, PCR normal bɛ se ka kɛ ADN dɔrɔn de kan.

2. PCR waati yɛrɛ la / PCR hakɛlama (Q-PCR) .

Min bɛ kɛ o la, o ye ko ADN kopiw bɛ kɛ tuma min na, fluorescent label kɛrɛnkɛrɛnnenw bɛ fara a kan. O taamasiyɛnw bɛ yeelen bɔ ni u sirilen don ADN laɲinilen na. O kɔfɛ, ni sɛgɛsɛgɛli wɛrɛ ma kɛ, sɛgɛsɛgɛli kɛbagaw bɛ se k’a ye waati yɛrɛ la ni ADN min bɛ tali kɛ o la, o bɛ sɔrɔ o sɛgɛsɛgɛli la. O dɔrɔn tɛ, u bɛ Se fana ka hakilina hakɛlama Sɔrɔ hakɛ min bɛ yen.

Fɛn min bɛ mɔgɔ hakili ɲagami, o ye ko mɔgɔ dɔw fana b'o Weele ko "RT-PCR". Nka a ni Reverse-Transcriptase PCR min kofɔra ka tɛmɛ, o tɛ kelen ye. Ani fana, nin Real-time PCR fɛɛrɛ in bɛ se ka kɛ ka fara Reverse-Transcriptase PCR kan min kofɔra ka tɛmɛ. O kɔfɛ, a bɛ wele ko Real-time RT-PCR (waati yɛrɛ la RT-PCR) . Nin RT-PCR fɛɛrɛ min bɛ kɛ waati yɛrɛ la, o bɛ kɛ ka nugula sɛgɛsɛgɛli kɛ walasa ka ARN banakisɛw sɔrɔ i n’a fɔ Covid-19 ani kunfilanitu.

PCR sɛgɛsɛgɛli jaabiw bɛ mun fɔ an ye?

Ni aw ye PCR sɛgɛsɛgɛli jaabi sɔrɔ, daɲɛw bɛ se ka kɛ danfara dɔɔni ye, ka kɛɲɛ ni aw sɛgɛsɛgɛra min na.

• A ka ca a la, ni aw ka jaabi ko "detected" walima "present", o kɔrɔ ye ko sɛgɛsɛgɛli ye fɛn min ɲini, o ye fɛn ye min bɛ se ka fɛn caman sɛgɛsɛgɛ walima ka banakisɛw sɔrɔ.
• Ni jaabi ko "a ma sɔrɔ", o kɔrɔ ye ko sɛgɛsɛgɛli ma se ka ADN walima ARN laɲinilen sɔrɔ min tun bɛ sɛgɛsɛgɛli la. Nka, ni banakisɛ dɔ donna, o bɛ se ka kɛ tuma dɔw la ko aw ye sɛgɛsɛgɛli kɛ joona kojugu banakisɛ sɔrɔcogo la. A bɛ se ka kɛ bana in bɛ aw la hali bi, nka a bɛ se ka kɛ banakisɛ walima banakisɛ tɛ se ka sɔrɔ PCR fɛ o waati la.

Covid-19 PCR jaabiw kɔrɔ fɔ cogo di?

Nin ye fɛn ye mɔgɔ caman bɛ min dɔn sisan.

• Ni COVID PCR jaabi ɲuman - o kɔrɔ ye ko i ka rapɔɔri ko "Detected" - o kɔrɔ ye ko a ka c’a la ko COVID-19 bana bɛ i la , walima i kɛnɛyara kɔsa in na COVID-19 la (tuma dɔw la PCR bɛ se ka to koɲuman na waati dɔ kɛnɛya kɔfɛ).
• Ni aw ye COVID PCR jaabi sɔrɔ min ma ɲɛ ("Not Detected"), o kɔrɔ ye ko a ka c’a la, COVID-19 tun tɛ aw la tuma min na aw ye nugu ta. Nka, i n’a fɔ n y’a fɔ cogo min na ka tɛmɛ, tuma dɔw la, banakisɛ dɔ daminɛni na, kɛrɛnkɛrɛnnenya la ni taamasiɲɛ tɛ i la, PCR sɛgɛsɛgɛliw bɛ se ka kɛ ko a ma ɲɛ. O la ni bana taamasiɲɛw bɛ aw la, dɔgɔtɔrɔ bɛ se ka aw laadi ko aw ka segin ka sɛgɛsɛgɛli kɛ tile damadɔ kɔfɛ.

A bɛ waati joli ta walasa ka PCR jaabiw sɔrɔ?

O fana bɛ ɲɔgɔn ta ka kɛɲɛ ni sɛgɛsɛgɛli min bɛ kɛ.

• A ka c’a la, nugulatumu sɛgɛsɛgɛli minnu bɛ kɛ ni banakisɛw ye minnu ka ca i n’a fɔ kunfilanitu ni COVID-19, olu bɛ lɛri damadɔ walima tile kelen kɛ walasa ka nɔ bɔ.
• Jɛnkulu sɛgɛsɛgɛli minnu bɛ kɛ ni joli sɛgɛsɛgɛli ni biyɔpisi fɛɛrɛw ye, olu bɛ se ka dɔgɔkun kelen walima ka tɛmɛ o kan walasa ka jaabi sɔrɔ barisa sɛgɛsɛgɛli kɛcogo ka gɛlɛn dɔɔni.

Aw ka kan ka taa dɔgɔtɔrɔso la tuma jumɛn?

Ni ɲininkali walima haminanko dɔw bɛ aw fɛ PCR sɛgɛsɛgɛli walima a jaabiw kan, aw ye aw jija ka kuma aw ka dɔgɔtɔrɔ fɛ. U bɛ se ka fɛn bɛɛ ɲɛfɔ i ye.

Cikan min nafa ka bon kosɛbɛ ka taa n’a ye so

Hali n'a y'a sɔrɔ "PCR test" daɲɛw barila ka don an ka don o don baro la ni san 2020 COVID-19 bana ye, tiɲɛ yɛrɛ la, a ye fɛɛrɛ nafamaba ye, dɔgɔtɔrɔw bɛ ka baara kɛ ni min ye kabini san tan caman. PCR bɛ dɛmɛ ka ADN walima ARN hakɛ misɛnninw dɔn ani k’u kopi kɛ, minnu bɛ se ka kɛ ka kɛnɛyako suguya caman sɛgɛsɛgɛ.

Hali ni o ka teli ka kɛ dɔgɔtɔrɔw fɛ, a bɛ se ka kɛ ko a man teli ka kɛ aw ka sɛgɛsɛgɛli kɛ. An b’o faamu. Nka min ka fisa, o ye ko a bɛ i farikolo yɔrɔ fitinin dɔrɔn de wajibiya, wa a ka c’a la, o kɛcogo bɛ miniti damadɔ dɔrɔn de ta. Ni ɲininkali, haminanko walima siga bɛ aw la o ko la, aw bɛ kuma aw ka dɔgɔtɔrɔ fɛ. U bɛna fɛn bɛɛ ɲɛfɔ i ye. Aw ka to kɛnɛya la!

` PCR sɛgɛsɛgɛli, ADN, ARN, jamu sɛgɛsɛgɛli, sɛgɛsɛgɛli, Covid-19, banakisɛ