An ka dɔn dɔrɔn immunophenotyping test kan min bɛ dɛmɛ ka kansɛribana banakisɛw dɔn.

N’a sɔrɔ aw ma `(Immunophenotyping)` daɲɛ mɛn fɔlɔ. Nka nin ye sɛgɛsɛgɛli ye min nafa ka bon kosɛbɛ, kɛrɛnkɛrɛnnenya la bana dɔw sɛgɛsɛgɛli la, misali la, jolidɛsɛ, jolidɛsɛ, ani kansɛri wɛrɛw. O la, bi an bɛna kuma nin kɔrɔbɔli in kan cogo nɔgɔman na, walasa aw ka se k’a faamu kosɛbɛ.

Immunophenotyping ye mun ye? An k’a faamu cogo nɔgɔman na.

O bɛ ɲɛ, sisan an k’a lajɛ nin daɲɛ `(Immunophenotyping)` kɔrɔ ye min ye. Hali n’o mankan bɛ i n’a fɔ dɔnniya kumasen dɔɔnin, tiɲɛ yɛrɛ la, maana in ka nɔgɔn kosɛbɛ.

Daɲɛ min ye ‘Immuno’ ye, o bɛ fɛn dɔ jira min bɛ tali kɛ an ka farikolo tangacogo la . Nin sɛgɛsɛgɛli in na, banakɔtaa dɔnbaga dɔ (dɔgɔtɔrɔ min ka baara ɲɛsinnen bɛ banaw sɛgɛsɛgɛli n’u furakɛli ma) bɛ baara kɛ ni farikolojɔlan suguya wɛrɛ ye min bɛ wele ko ‘anticorps’ min bɛ bɔ an yɛrɛ ka farikolo tangalanw na walasa ka farikolojɔlifɛn kɛrɛnkɛrɛnnenw ɲini minnu bɛ wele ko ‘antigènes’ an ka selilɛriw kɔnɔ. Aw ye miiri nin ‘banakisɛfagalanw’ la i n’a fɔ kilisi, ani ‘banakisɛfagalanw’ i n’a fɔ dakun. I n’a fɔ dakun min bɛ bɛn kilisi bɛɛ ma, ‘banakisɛfagalan’ kɛrɛnkɛrɛnnen dɔ bɛna nɔrɔ ‘banakisɛfagalan’ kɛrɛnkɛrɛnnen dɔrɔn de la. O kɔrɔ ye ko ɲɔgɔndan sugu dɔ bɛ u fla ni ɲɔgɔn cɛ.

‘Fɛnotipi’ daɲɛ bɛ tali kɛ kɛnɛmafɛnw na minnu bɛ se ka kɔlɔsi . O la, ni o `(antigène)` sirilen don `(antigène)` la min bɛ tali kɛ a la, banakɔtaa dɔnbaga b’a dɔn n’o `(antigène)` bɛ o selilɛri in na walima n’a tɛ a la ka da a bɛ taamasiɲɛ minnu jira.

N’an y’a fɔ cogo nɔgɔman na, nin `(Immunophenotyping)` sɛgɛsɛgɛli in bɛ nin `(antigènes)` taamasiɲɛ ninnu lajɛ an ka farikolokisɛw sanfɛ, k’a dɔn n’o farikolokisɛw ye farikolokisɛ kɛnɛmanw ye walima ni kansɛri bɛ u la. Ani fana, nin `(antigènes)` ninnu bɛ se k’a dɔn kansɛribanakisɛ suguya min don.

Nin (Immunophenotyping) sɛgɛsɛgɛli in bɛ kɛ tuma jumɛn?

Ko damadɔ bɛ yen dɔgɔtɔrɔ bɛ se ka nin sɛgɛsɛgɛli in fɔ minnu na.

Miiri k’a filɛ i terikɛ dɔ, an k’a fɔ ko Nimal, a sɛgɛnnen don kosɛbɛ ani a sɛgɛnnen don kabini tile damadɔ. Dɔgɔtɔrɔ ye joli sɛgɛsɛgɛli kɛ tuma min na (``Joli jate dafalen'', walima ``CBC''), a y'a ye ko a joli kuru finmanw hakɛ ka ca cogo la min tɛ bɛn. O kɔ, dɔgɔtɔrɔ bɛ se ka sigasiga ko o bɛ se ka kɛ joli kansɛri ye. O waati de la a bɛna ladilikan di ka nin ``Immunophenotyping'' sɛgɛsɛgɛli in kɛ walasa ka kunnafoni caman sɔrɔ min bɛ sen na tigitigi.

O kɔrɔ ye ko:

• Ni joli jate dafalen (`(CBC)`) jaabiw y’a jira ko aw ka joli kuru finmanw hakɛ ka ca cogo la min tɛ bɛn.
• Ni siga bɛ joli kansɛri suguya dɔw la, misali la jolidɛsɛ walima jolidɛsɛ, o bɛ se ka dɛmɛ don ka gɛrɛ a sɛgɛsɛgɛli la dɔɔni.

Ka fara o kan, ɲininikɛlaw fana bɛ baara kɛ ni nin fɛɛrɛ in ye min ye ``Immunophenotyping'' ye walasa ka kansɛribanakisɛw bɛ jaabi cogo min na ``antigène'' taamasiyɛn kɛrɛnkɛrɛnnenw na, kɛrɛnkɛrɛnnenya la ``immunotherapy'' sɛgɛsɛgɛli.

Nin kɔrɔbɔli in bɛ baara kɛ cogo di?

Nin `(Immunophenotyping)` sɛgɛsɛgɛli fanba bɛ kɛ laboratuwari la, farikolo yɔrɔ walima ji talen kɔfɛ aw la. Ni an y’a fɔ k’a jɛya, i ka kan ka min kɛ, o ye ka misali di, wa laboratuwari dɔnnikɛlaw de bɛ tɔ kɛ.

Dɔgɔtɔrɔ fanba bɛ laboratuwari fɛɛrɛ dɔ kɛ min bɛ wele ko ``Flow Cytometry'' walasa ka nin sɛgɛsɛgɛli in kɛ. Ka fara o kan, tuma dɔw la, fɛɛrɛ dɔ fana bɛ kɛ min bɛ wele ko ``Immunohistochimie - IHC''. An ka nin fila bɛɛ lajɛ.

Fɛɛrɛba fila bɛ yen...

(Flow Cytometry) fɛɛrɛ (fɛɛrɛ min bɛ kɛ ka caya) .

Aw bɛ baara kɛ ni nin fɛɛrɛ in ye, aw bɛ se ka joli kurubaw walima joli kuru minnu bɔra farikolo yɔrɔw la, olu sɛgɛsɛgɛ. A bɛ baara kɛ cogo min na, o filɛ nin ye:

• Sɛgɛsɛgɛli lajɛli : A ka ca a la o bɛ kɛ ni kolotuguda sɛgɛsɛgɛli ye ka bɔ kolotugudaw la, walima joli dɔɔni dɔɔni. Tuma dɔw la, a bɛ se ka ta ka bɔ kɔkoloji la walima ji la min bɛ kɔnɔbara la.
• Sɛgɛsɛgɛli lakanani : Laboratowari baarakɛlaw bɛ sɛgɛsɛgɛli labɛn ``Immunophenotyping'' sɛgɛsɛgɛli kama. U bɛ fɛn dɔw fara a kan walasa farikolokisɛw kana sa.
• Fàrali (Antibody): O kɔ fɛ, u ye ``anticorps`` minnu Dabɔ walasa k'u siri ``antigène`` la u b'a fɛ ka min Sɔrɔ, u bɛ olu Fàra ɲɔgɔn kan. O ``anticorps`` ninnu bɛ nɔrɔ ``fluorescent compound`` walima ``immunofluorescence marker`` la. O taamaʃyɛn in sababu la, ni ``anticorps`` sirilen don ``antigène`` la, seliluw bɛ yeelen bɔ.
• Baara kɛli (Flow Cytometry): Sisan, sɛgɛsɛgɛli min labɛnna ni o `(anticorps)` ye, o bɛ don masin dɔ kɔnɔ min bɛ wele ko `(Flow Cytometer)`. Nin masin in bɛ ni sensɛri kɛrɛnkɛrɛnnenw ye (`detectors`), minnu bɛ se ka yeelen min bɛ bɔ o `(immunofluorescence marker)` la, o dɔn. O la, nin `(Flow Cytometer)` bɛ selilu hakɛ suman minnu sirilen bɛ `(antigène-antibody)` (i n'a fɔ yeelen bɔ).

(Immunohistochimie - IHC) fɛɛrɛ

Immunohistochimie ye fɛɛrɛ ye min bɛ se ka antigène kɛrɛnkɛrɛnnenw sɔrɔli dɔn farikolo yɔrɔ dɔ la. Fɛɛrɛ minnu bɛ kɛ laboratuwari la, olu ni flow cytometry ta bɛ tali kɛ ɲɔgɔn na kosɛbɛ. Danfaraba min bɛ u ni ɲɔgɔn cɛ, o ye ko IHC la, anzimu dɔ bɛ fara farikolo tangalanw kan sanni ka kɛ fɛnɲɛnamafagalan ye min bɛ yeelen bɔ. Nin anzimu in bɛ farikolokisɛw nɔgɔ ni kulɛri kɛrɛnkɛrɛnnen ye ni antijeni bɛ yen. Ni banakɔtaa dɔnbaga ye nɔgɔ ye mikroskɔpu fɛ, o kɔrɔ ye ko banakisɛfagalan bɛ farikolokisɛw la.

Nin (Immunophenotyping) sɛgɛsɛgɛli in dan ye mun ye?

Nafa min ka bon kosɛbɛ, o ye ko laboratuwari bɛɛ min bɛ nin sɛgɛsɛgɛli in kɛ, o tɛ ni `` taamacogo jɔnjɔnw'' kelen ye.O la, tuma dɔw la, fɛn dɔw bɛ se ka ɲɔgɔn falen dɔɔnin a jaabiw la.

Nka fɛn kelen min ka kan ka to i hakili la. Dɔgɔtɔrɔw tɛ bana dɔ sɛgɛsɛgɛ ka da nin `(Immunophenotyping)` jaabiw dɔrɔn kan. U b’a suma ni fɛn caman wɛrɛw ye, i n’a fɔ aw ka bana taamasiɲɛw ani sɛgɛsɛgɛli wɛrɛw jaabiw, walasa ka se ka dantigɛli kɛ. O kɔrɔ ye ko nin sɛgɛsɛgɛli in dɔrɔn t’a fɔ aw ye ko kansɛri bɛ aw la. O ye ja belebeleba dɔ dɔrɔn de ye, sɔrɔyɔrɔ kelen dɔrɔn min bɛ se k’i dɛmɛ ka fɛn caman dɔn i ka kɛnɛyako kan.

O nɔ bɛ kɛ cogo di? U bɛ mun fɔ an ye?

Aw ka dɔgɔtɔrɔ bɛna a ɲɛfɔ aw ye aw ka jaabiw kɔrɔ ye min ye ka da banakɔtaa dɔnbaga ka sɛgɛsɛgɛli kan. ``Immunophenotyping'' sɛgɛsɛgɛli jaabiw tɛ ``ɲuman'' walima ``jugu'' dɔrɔn ye. A bɛ banakɔtaa dɔnbaga dege walasa ka ``dantigɛliw'' faamuya minnu bɛ sɔrɔ ``Flow Cytometer'' fɛ, ka ɲɛsin sɛgɛsɛgɛli ma, ani k'a bɛ min fɔ, k'o kɔrɔ fɔ ka ɲɛ.

O kunnafoniw fɛ, a bɛ fɛnw sɛgɛsɛgɛ i n’a fɔ :

• `Joli kuru finman suguya` ye mun ye?
• U bɛ yiriwali siratigɛ jumɛn na?
• Aw bɛ antijeni min ɲini, o bɛ ni selilu joli ye?

Banakisɛfagalan bɛ aw ka sɛgɛsɛgɛli kɛ kunnafoniw suma ni mɔgɔ kɛnɛmanw ka kunnafoniw ye minnu tɛ kansɛri sɔrɔ, ka fara kansɛribana suguya kɛrɛnkɛrɛnnen dɔ bɛ mɔgɔ minnu na. Ni a bɛ baara kɛ ni o misaliw ye ani sɛgɛsɛgɛli wɛrɛw jaabiw ye, a b’a dɔn kansɛri min kofɔlen don, o bɛ se ka kɛ i la cogo min na.

Tuma jumɛn na ne ka kan ka n ka dɔgɔtɔrɔ wele?

Ni ɲininkali walima haminanko dɔw bɛ aw fɛ aw ka jaabiw kan walima u kɔrɔ kan, aw ye aw jija ka dɔgɔtɔrɔ wele . Immunophenotyping ye laboratuwari sɛgɛsɛgɛli gɛlɛn ye min bɛ farikolokisɛw cogoyaw lajɛ. Nka aw ka dɔgɔtɔrɔ bɛ se k’a ɲɛfɔ a bɛ nɔ bila aw ka kɛnɛya la cogo min na ni kuma nɔgɔmanw ye.

N’a sɔrɔ aw ma dimi foyi sɔrɔ, wa aw kabakoyara k’a ye ko aw ka joli kuru finmanw ka ca joli sɛgɛsɛgɛli kɛcogo kɔrɔ la (``CBC''). Walima a bɛ se ka kɛ ko i fari tɛ a la kabini waati jan. Nka, o ``CBC'' jaabiw ye fɛɛrɛ fɔlɔ dɔrɔn de ye walasa k'a dɔn min bɛ sen na, o la i bɛna muɲu.

Kunnafoni nafama caman bɛ aw ka selilɛriw kɔnɔ aw ka kɛnɛyako kan. Immunophenotyping ye sɛgɛsɛgɛli ye min bɛ aw ka farikolokisɛw lajɛ ani ka aw ka kɛnɛyako fɔ aw ye. Nin waati suguw la, ka a nɔw makɔnɔ, o bɛ se ka kɛ siranfɛn ye tiɲɛ na ani ka jɔrɔ. Ni aw mago bɛ dusukunnataw ta fan fɛ dɛmɛ na, aw bɛ kuma aw ka dɔgɔtɔrɔ fɛ o ko la. Ani fana, ni ɲininkali dɔw bɛ aw fɛ sɛgɛsɛgɛli jaabiw kan, aw kana siga ka ɲininkali kɛ.

Fɛn minnu nafa ka bon kosɛbɛ, an ka kan ka minnu kalan o la

O bɛ ɲɛ, o la, an ye fɛn minnu nafa ka bon kosɛbɛ, an ka kan ka minnu hakili to nin `(Immunophenotyping)` sɛgɛsɛgɛli in na, an ye minnu fɔ bi, an ka olu lajɛ.

• Nin ye laboratuwari sɛgɛsɛgɛli kɛrɛnkɛrɛnnen ye. A bɛ dɛmɛ don kosɛbɛ kansɛriw sɛgɛsɛgɛli la i n’a fɔ jolidɛsɛ ni jolidɛsɛ.
• O bɛ Kɛ ni farikolojɔlan kɛrɛnkɛrɛnnenw dɔnni ye minnu bɛ Weele ko ``antigènes'' an ka farikolokisɛw sanfɛ.
• Fɛɛrɛ fila minnu bɛ kɛ ka caya, olu ye ``Flow cytometry'' ani ``Immunohistochemistry - IHC'' ye.
• O jaabiw ka gɛlɛn dɔɔni, o la banakɔtaa dɔnbaga dɔ b’u sɛgɛsɛgɛ.
• Aw ye nin to aw hakili la: Mɔgɔ si tɛ se k’a fɔ aw ye ko kansɛri bɛ aw la ni aw ye nin sɛgɛsɛgɛli in jaabiw lajɛ dɔrɔn. O ye bana sɛgɛsɛgɛli kɛcogo yɔrɔ kelen dɔrɔn de ye.
• Ni aw bɛ ɲininkali dɔw kɛ a nɔfɛkow kan, aw kana siran ka dɔgɔtɔrɔ ɲininka. U bɛ yen walisa k’i dɛmɛ.

` Immunophenotyping, kansɛri, jolidɛsɛ, farikolojidɛsɛ, joli sɛgɛsɛgɛli, farikolokisɛw, antigènes