Bi an bɛna kuma an farikolo lakananifɛn fitininw kan: Anticorps polyclonaux!

Yala aw delila ka ‘Anticorps polyclonaux’ daɲɛw mɛn wa? N’a sɔrɔ i ye dɔgɔtɔrɔ dɔ ka kuma mɛn, walima i y’a kalan gafe walima kunnafonisɛbɛn dɔ kɔnɔ yɔrɔ dɔ la. Hali n’a bɛ se ka kɛ ko nin ye dɔnniya daɲɛ ye dɔɔnin ani min faamuyali ka gɛlɛn, tiɲɛ yɛrɛ la, a nafa ka bon kosɛbɛ, kosɛbɛ an farikolo kɛnɛyako la ani k’an tanga banaw ma. O la bi an bɛna kuma nin banakisɛfagalan poliklɔni ninnu ye mun ye, u bɛ baara kɛ cogo min na an farikolo kɔnɔ, u nafa ka bon an ma cogo min na, ani u nafa n’u dɛsɛ ye mun ye, nɔgɔya la, i n’a fɔ an b’a fɔ an teri ɲuman walima an somɔgɔw ye. Labɛn?

N’an y’a fɔ cogo nɔgɔman na, nin banakisɛfagalan poliklɔniw ye mun ye?

O bɛ ɲɛ, fɔlɔ an k’a faamu ka jɛya ko nin Banakisɛfagalanw ye mun ye. Yala aw hakili b’a la, ni an farikolo banana, o kɔrɔ ye ko ni fɛn dɔ donna i n’a fɔ tumu, yɔrɔ kɛrɛnkɛrɛnnenw bɛ an fari la minnu b’a kɛlɛ. An bɛ min wele ko anticorps (banakisɛfagalanw). Olu bɛ iko garadi fitininw, walima sɔrɔdasiw, an farikolo la. O farikolojɔlifɛn minnu bɛ wele ko farikolo tangalanw, olu dilannen don an farikolo fɛ kɛrɛnkɛrɛnnenya la walasa ka banaw ni tumuw kɛlɛ ani k’an tanga.

Sisan, o banakisɛfagalanw bɛ bɔ farikolokisɛ suguya kɛrɛnkɛrɛnnen dɔ la min bɛ wele ko `(Plasma B cellules)`. Daɲɛ ``polyclonal'' kɔrɔ ye ``ka bɔ suguya caman na'' (poly = caman, clonal = bɔnsɔnw/suguya). O kɔrɔ ye ko nin farikolojɔlifɛn poliklɔni ninnu tɛ suguya kelen ye, u tɛ bɔ nɔgɔ kelen na. U ye farikolo tangalanw dalajɛlen ye minnu bɛ bɔ joli-sira-funu-banakisɛw bɔnsɔnw na (`banakisɛw`), wa minnu ni ɲɔgɔn tɛ kelen ye dɔɔni. Miiri k’a filɛ i n’a fɔ sɔrɔdasi kulu min bɔra kɛlɛbolo kelen ka kɛlɛbolow la minnu tɛ kelen ye.

O kɔfɛ, o banakisɛfagalan kelen-kelen bɛɛ, walima sɔrɔdasi suguya wɛrɛw, bɛ banakisɛfagalan kelen yɔrɔ wɛrɛw dɔn. Sisan aw bɛ ɲininkali kɛ ko: "Antigène ye mun ye?" Aw k’a miiri i n’a fɔ ‘label’ walima ‘drapo’ fitinin dɔ bɛ tumu, mugu walima farikolojɔlifɛn dɔ kan min tɛ dɔn an fari la. Ni an ye nin taamasiyɛn in lajɛ, an farikolo tangalan, an farikolo lafasalitɔn, b’a dɔn ko an yɛrɛ (`yɛrɛ`) don walima jamana wɛrɛ jugu (`an yɛrɛ tɛ`). A bɛ iko jugu ka fini, walima jugu ka kɛlɛbolo ka darapo.

O la, nin farikolojɔlifɛn poliklɔni ninnu ye sɔrɔdasi suguya wɛrɛw ye (banakisɛfagalan) minnu bɛ se ka taamasiyɛn suguya wɛrɛw dɔn, cogoya wɛrɛw (an b’o wele dɔnniya siratigɛ la ko `epitopes` ) jugu kelen (antigène) ka fini walima a darapo kan. Yala i y’a faamu wa? A bɛ iko sɔrɔdasi setigiw ka kulu min bɛ se ka binkanni kɛ jugu kelen kan ka bɔ fan wɛrɛw la.

Ni hadamadenw ni bagan wɛrɛw banana, o kɔrɔ ye ko ni banakisɛ dɔ donna u la, o banakisɛfagalan caman bɛ sɔrɔ u farikolo la u yɛrɛ la. Nka, banakisɛfagalanw (polyclonal anticorps) minnu bɛ kɛ furakɛli ɲininiw na ani furakɛli dɔw la, olu ka teli ka sɔrɔ baganw fɛ i n’a fɔ laɲɔw, sagaw ani bakɔrɔnw. Tuma dɔw la, u bɛ sɔrɔ fana mɔgɔw joli la minnu kɛnɛyara ka bɔ bana dɔw la.

Nin farikolojɔlifɛn poliklɔniw bɛ baara kɛ cogo di tiɲɛ na?

Sisan an k’a lajɛ nin farikolojɔlifɛn poliklɔni ninnu bɛ baara kɛ cogo min na an farikolo la, u bɛ kɛlɛ kɛ cogo min na. Ni banakisɛ, banakisɛ walima banakisɛ wɛrɛ (banakisɛ) donna aw fari la, aw farikolo tangalanw bɛ farikolo tangalanw dilan walasa k’u kɛlɛ.

An ye antijeni minnu kofɔ ka tɛmɛ, olu bɛ o banakisɛ ninnu sanfɛ. Banakisɛfagalanw (an ka sɔrɔdasiw) bɛ o banakisɛfagalanw dɔn ka u siri u la. Banakisɛfagalan kelen-kelen bɛɛ bɛ siri banakisɛfagalan kɛrɛnkɛrɛnnen kelen dɔrɔn de la. O kɔrɔ ye ko kilisi kelen-kelen bɛɛ bɛ bɛn dakun kɛrɛnkɛrɛnnen kelen dɔrɔn ma. Ni banakisɛfagalan dɔ sirilen don banakisɛfagalan dɔ la, a bɛ taamasiyɛn ci farikolo tangalan tɔ ma, k’a fɔ ko “jugu dɔ filɛ nin ye, sisan a tiɲɛ.”

Nka, hali ni banakisɛfagalan kelen bɛ yen, yɔrɔ caman bɛ se ka sɔrɔ banakisɛfagalan bɛ nɔrɔ yɔrɔ minnu na, i n’a fɔ tori ka kilisi dingɛ. An bɛ o siriyɔrɔ kɛrɛnkɛrɛnnenw Weele ko `(epitopes)`. O la, banakisɛfagalan poliklɔni minnu bɛ aw joli la, walima furaji caman (`serums`) minnu bɛ di furakɛli kama, olu ye banakisɛfagalan suguya caman dalajɛlen ye. Nin farikolojɔlan kelen-kelen bɛɛ bɛ se ka `(epitopes)` (siriyɔrɔw danfaralenw) danfaralenw dɔn ani ka u siri o banakisɛfagalan kan. O kɔrɔ ye ko a bɛ i n’a fɔ sɔrɔdasi kulu danfaralen damadɔ minnu bɛ baara kɛ waati kelen na walasa ka jugu kelen minɛ.

Miiri k’a filɛ, juguba dɔ be yen. N’i ​​bɛ se k’o jugu minɛ yɔrɔ kelen dɔrɔn na, nka yɔrɔ caman na siɲɛ kelen, i n’a fɔ a bolo, a sen, a kunkolo ani a kɔla, i bɛ se ka jugu minɛ ani k’a kɛ seko dɛsɛ ye, o ka bon kosɛbɛ. O cogo de la banakisɛfagalanw (polyclonal anticorps) bɛ baara kɛ. Banakisɛfagalan suguya wɛrɛw (`lineages`) bɛ cogo caman di farikolo tangalanw ma walasa u ka tumuw dɔn, ka u bin u kan, ani ka u minɛ. O b’a to an farikolo lafasaliw bɛ barika sɔrɔ ka tɛmɛn fɔlɔ kan.

An bɛ baara kɛ ni farikolojidɛsɛbanakisɛw ye minnu bɛ wele ko polyclonal anticorps (banakisɛfagalanw) minnu bɛ kɛ mun na?

Nin farikolo tangalan poliklɔni ninnu, an bɛ minnu dilan an farikolo la an yɛrɛ la, olu fana bɛ kɛ furakɛli la baara nafama caman na. An k’a lajɛ u ye fɛn minnu ye:

• Bana sɛgɛsɛgɛli : Laboratowari sɛgɛsɛgɛli dɔw bɛ yen, aw bɛ se ka minnu mɛn, i n’a fɔ ELISA sɛgɛsɛgɛli ani Immunohistochemistry sɛgɛsɛgɛli. Nin sɛgɛsɛgɛliw bɛ kɛ ni banakisɛfagalan caman ye walasa ka banakisɛ suguya caman dɔn (misali la sidabanakisɛ, sugunɛbilenni) ani kansɛri. O kɔrɔ ye ko u bɛ se k’a dɔn ni joli la, banakisɛfagalan dɔ bɛ yen min kɛrɛnkɛrɛnnen don bana in ma.

• Dɔnniya ɲininiw: Sɛgɛsɛgɛlikɛlaw bɛ baara kɛ ni o banakisɛfagalan caman ye walasa k’a faamu an farikolo bɛ baara kɛ cogo min na, farikolokisɛ suguya caman bɛ baara kɛ cogo min na, banaw bɛ an minɛ cogo min na, ani ka fura kuraw sɔrɔ. U bɛ i n'a fɔ baarakɛminɛn minnu bɛ dɔnnikɛlaw Dɛmɛ ka fɛnw "ye" minnu tɛ Ye.

• Banakisɛfagalanw ni kɛnɛyako dɔw furakɛli walima u kunbɛnni:Banakisɛfagalan caman bɛ se ka fura tata hakɛ dɔw furakɛ, ka dɔw furakɛ, ka sa baga ka kinni kɛ, ani ka bana dɔw bali minnu bɛ sɔrɔ joli fɛ.

Furakɛli jumɛnw bɛ kɛ ni banakisɛfagalanw ye minnu bɛ wele ko polyclonal anticorps?

An ka furakɛli dɔw lajɛ kosɛbɛ ni banakisɛfagalanw ye minnu bɛ wele ko polyclonaux:

• Banakisɛfagalan dɔw bɛ sɔrɔ banakisɛfagalanw na minnu bɛ wele ko polyclonale. Olu bɛ Kɛ ni sa baga dɔɔni pikiri ye baganw na, i n’a fɔ sow, ka sɔrɔ ka farikolo tangalanw saniya minnu bɛ Bɔ o baganw fɛ. Ni sa y’i kin, banakisɛfagalan minnu bɛ banakisɛfagalan na, olu bɛ taa baga in kɛ fɛn ye min tɛ se ka kɛ.

• Plasma convalescent: N’a sɔrɔ aw ye nin mɛn COVID-19 bana waati la. Tuma min na banakisɛ juguman dɔw bɔra, joli-sira-funu (joli yɔrɔ jilama) min bɛ bɔ mɔgɔw joli la minnu kɛnɛyara o bana in na, o bɛ di banabagatɔw ma. Sabula o joli la, farikolojidɛsɛbanakisɛ caman bɛ yen, u farikolo ye minnu dilan o bana kama. Ni o banakisɛfagalanw donna banabagatɔ fari la, u bɛ dɛmɛ don ka bana in kɛlɛ.

• Digoxin immune fab: Nin ye boloci ye min bɛ di ni mɔgɔ dɔ ye dusukun fura digoxin tata hakɛ caya kojugu. Nin fana na, banakisɛfagalanw (polyclonal anticorps) bɛ sɔrɔ minnu dilannen don digoxin molecule (digoxin molecule) kama.

• Rho(D) immune globulin : O fana bɛ wele ko `RhIG`, wa aw bɛ se k’a ye fana ko a sɛbɛnnen don ko `Rh 0 (D)`. O bɛ di denba kɔnɔmaw ma minnu joli suguya ye `Rh-negatif` ye. O kun ye ko ni ba joli ye `Rh-negatif` ye, ni den joli ye `Rh-positif` ye, ba farikolo bɛ se ka banakisɛfagalanw sɔrɔ den joli la. Hali ni o tɛ se ka nɔ bila den fɔlɔ la, ni den `Rh-positif` filanan bangera, o banakisɛfagalanw bɛ se ka kojugu kɛ den na. O bɛ wele ko `(Rhesus isoimmunisation)` (Rhesus incompatibilité). Nin ye `Rho(D)` boloci bɛ di walasa ka a kunbɛn. A bɛ di fana ka kɛ fura ye `(Chronic immune thrombocytopenie)` walima `(ITP)`, bana min na joli segin furakisɛw bɛ dɔgɔya.

Danfara jumɛn bɛ farikolo tangalan poliklɔni ni banakisɛfagalan kelen cɛ?

O bɛ ɲɛ, sisan i bɛ se ka ɲininkali fitinin dɔ kɛ. A suguya dɔ fana bɛ yen min bɛ wele ko ‘Monoclonal Anticorps’, danfara jumɛn bɛ ninnu ni nin banakisɛfagalan poliklonali ninnu cɛ? U fila bɛɛ ye farikolo tangalanw ye, wa u fila bɛɛ bɛ banakisɛfagalanw laɲini. Nka danfara nafama dɔw be yen.

N’an y’a fɔ cogo nɔgɔman na:

• Banakisɛfagalan caman (Polyclonal Anticorps) : Olu bɛ bɔ B-cell bɔnsɔnw na minnu tɛ kelen ye. O la u bɛ se ka `(epitopes)` (siriyɔrɔw) suguya caman dɔn ani ka u siri antijeni kelen kan. A bɛ iko sɔrɔdasi kulu min ka baara kɛrɛnkɛrɛnnenw tɛ kelen ye ani u ka marifaw tɛ kelen ye. U bɛ bin jugu kelen kan ka Bɔ yɔrɔw la minnu tɛ kelen ye.

• Banakisɛfagalan kelen-kelenw : Olu ye banakisɛfagalan kelen ``kopi tigitigiw`` ye. O kɔrɔ ye ko u bɛ dilan ni B-cell bɔnsɔn kelen ye. O la, u bɛ ``epitope`` kelen dɔrɔn de Dɔn k'u siri. I n’a fɔ sɔrɔdasi minnu dilannen don ni fɛn kelen ye, n’u bɛ marifa suguya kelen de kɛ, aw bɛ bin laɲini kelen dɔrɔn kan.

Furakɛcogo la, a ka ca a la banakisɛfagalanw bɛ sɔrɔ baganw joli la i n’a fɔ laɲɔ, bakɔrɔn ani saga. Tuma dɔw la, u bɛ sɔrɔ fana hadamadenw fɛ (misali la, banabagatɔ minnu kɛnɛyara, olu ka joli-sira-funu). Banakisɛfagalan kelen-kelenw bɛ sɔrɔ fɔlɔ baganw joli la, o kɔfɛ, u bɛ segin ka kɛ laboratuwari kɔnɔ. Sisan, banakisɛfagalanw bɛ kɛ ka caya ka tɛmɛ banakisɛfagalan caman kan furakɛli la, kɛrɛnkɛrɛnnenya la banaw la i n’a fɔ kansɛri, barisa u kɛrɛnkɛrɛnnen don u laɲinicogo la.

Nafa jumɛnw bɛ banakisɛfagalanw na minnu bɛ wele ko polyclonal anticorps?

O la, nin banakisɛfagalan poliklɔni ninnu nafa ye mun ye? Mun na an bɛ baara kɛ n’u ye hali bi?

• A dilanni musakaw ka dɔgɔ kosɛbɛ. A sɔngɔ ka gɛlɛn ka ninnu dilan ka tɛmɛ banakisɛfagalanw dilanni kan minnu bɛ wele ko monoclonal anticorps.
• O bɛ se ka antigènes (banakisɛfagalanw) dɔn sɛgɛsɛgɛli dɔ la ni u hakɛ ka dɔgɔn kosɛbɛ, hali ni u bɛ sɔrɔ hakɛ dɔgɔmanba la. Ikomi banakisɛfagalan suguya wɛrɛw bɛ baara kɛ ɲɔgɔn fɛ, hali ni kelen ma sɔrɔ, a ka ca a la ko dɔ wɛrɛ bɛna minɛ.
• Se bɛɛ lajɛlen ka antijeni dɔ dɔn, o ka bon. Ikomi banakisɛfagalan caman bɛ yen minnu bɛ se ka siri ``epitope'' suguya wɛrɛw la, banakisɛfagalan dɔ dɔnni seko ka bon, hali n’a cogoya tɛ kelen ye dɔɔni.

Yala nafa dɔw bɛ yen o tuma na wa?

Nafa ni dɛsɛ bɛ nin banakisɛfagalan poliklɔni ninnu na. A ka ɲi k’a dɔn u ye min ye.

• Ikomi ninnu bɛ bɔ sɔrɔyɔrɔ la (adamadenw walima baganw joli), u jogo ni u kɛcogo tɛ kelen ye ka bɔ kulu kelen na ka taa kulu wɛrɛ la, u tɛ i n’a fɔ fura minnu dilannen don laboratuwari kɔnɔ. O kɔrɔ ye ko danfara fitini bɛ se ka kɛ banakisɛfagalanw ɲagaminen ni ɲɔgɔn cɛ min bɛ bɔ siɲɛ kelen ani ɲagami min bɛ bɔ siɲɛ wɛrɛ. O la, a nafa fana bɛ se ka ɲɔgɔn ta dɔɔni.
• Ni farikolojidɛsɛbanakisɛ kelenw bɛ yen, farati ka bon ka ɲɛsin ɲɔgɔndɛmɛ ma. O kɔrɔ ye ko a ka ca a la ko antijeni laɲinilen bɛ siri antijeni wɛrɛ la (an t’a fɛ min). O bɛ se ka gɛlɛyaw lase sɛgɛsɛgɛliw walima ɲininiw ma. Misali la, nkalon pozitifw bɛ se ka kɛ. O kɔrɔ ye ko bana tɛ mɔgɔ min na, o bɛ se ka nɔ sɔrɔ min bɛ i n’a fɔ bana in bɛ a la. O la, ni jaabi ɲuman seginna ka na, i n’a fɔ banakisɛ jugumanw na, dɔgɔtɔrɔw bɛ se ka sɛgɛsɛgɛli caman kɛ walasa k’a dɔn.
• Ni baganw ka banakisɛfagalanw (polyclonal anticorps) kɛra furakɛli la, faritanabana walima kɔlɔlɔ wɛrɛw farati ka bon, barisa o baganw ka farikolojɔli dumuniw ye dunan ye an farikolo la.

A laban na, fɛn nafamabaw minnu ka kan ka to i hakili la

O la, n’a sɔrɔla aw y’a faamu sisan ko farikolo tangalan poliklɔniw ye an farikolo farikolo tangacogo yɔrɔ nafamaba ye, wa u nafa ka bon furakɛli dɔnniya la ɲininiw, bana sɛgɛsɛgɛli ani furakɛli kɛrɛnkɛrɛnnen dɔw kama.

A ka c’a la, ninnu bɛ kɛ ɲininiw ni bana sɛgɛsɛgɛliw la. Nka ni aw kɔnɔma don ani ni joli suguya Rh-negatif bɛ aw la (aw ka dɔgɔtɔrɔ bɛna o fɔ aw ye), ni banakisɛ jugumanba dɔ bɛ aw la (i n’a fɔ kanjabana), ni sa ye aw kin, walima ni bana dɔ bɛ aw la i n’a fɔ farikolo tangalanw ka jolidɛsɛ basigilen (ITP), aw bɛ se ka fura dɔ di aw ma, farikolo tangalan caman bɛ min kɔnɔ.

Ni ɲininkali wɛrɛw walima haminanko wɛrɛw bɛ aw fɛ o ko la, aw kana siga ka dɔgɔtɔrɔ walima dɔgɔtɔrɔso baarakɛla ɲininka. U bɛna o kow ɲɛfɔ i ye ka ɲɛ. Sabula ka kunnafoni sɔrɔ i ka kɛnɛyako kan ani i bɛ furakɛli minnu kɛ, o ye fɛɛrɛ ɲumanw dɔ ye walasa ka to kɛnɛya la ani ka to nisɔndiya la!

` Banakisɛfagalan caman, Banakisɛfagalanw, farikolo tangalanw, Banakisɛfagalanw, Epitope, Sɛgɛsɛgɛli, Furakɛli