Ðe ɖɔktaa do Gram Stain dodokpɔ na wòa? (Gram Stain) Mina míanya nu tso esia ŋu bɔbɔe!

Ðe kɔdzidɔ si medzona le ŋkeke ʋɛ aɖewo megbe o, alo aɖuɖɔtoedɔléle ƒe dzesi siwo ɖea fu na wò la le ŋuwòa? Ðewohĩ esi nèyi ɖɔkta gbɔ la, eŋlɔ dodokpɔ aɖe si woyɔna be ‘Gram Stain’ na wò. Ŋkɔ sia ate ŋu aɖi nusi wɔ nuku vie na wò. Gake le nyateƒe me la, esia nye dodokpɔ vevi aɖe ŋutɔ, si wowɔna kabakaba, si wozãna tsɔ dea dzesi dɔlékuiwo le míaƒe ŋutilã me. Eyata, mina míaƒo nu tso esia ŋu bɔbɔe ŋutɔ, le mɔ si gɔme nàte ŋu ase nu.

Nukae nye Gram Stain ko?

Kpuie ko la, Gram stain nye dodokpɔ si wowɔna le dodokpɔxɔ me. Koŋ la, edia dɔlékuiwo le wò ŋutilã ƒe akpa aɖe si dɔlékui aɖe le. Tsɔe be abi aɖe le ŋuwò si le sisim. Emegbe woate ŋu aɖo abi ma ƒe kpɔɖeŋu sue aɖe ɖa hena dodokpɔ sia wɔwɔ.

Viɖe gãtɔ siwo le dodokpɔ sia ŋu dometɔ ɖekae nye be dodokpɔa me tsonuawo dzena kaba vie. Esia wɔnɛ be ɖɔktaa te ŋu nyaa kaba nenye be dɔlékui aɖe le ŋuwò eye ne ele nenema la, ekpɔnɛ be dɔlékui ƒomevi ka kee wònye. Esia kpena ɖe ɖɔktaa ŋu wòkpɔa atike siwo wòana wò ƒe susu vevi aɖe.

Menye dɔlékui siwo katã le míaƒe ŋutilã me ye gblẽ o. Wo dometɔ aɖewo nye dɔlékui nyui siwo kpena ɖe mía ŋu. Gake dɔlékui ƒomevi aɖewo hea dɔlélewo kple dɔlékuiwo vɛ na mí. Gram Stain dodokpɔa kpena ɖe mía ŋu míedea dzesi dɔlékui siwo gblẽa nu le ame ŋu siwo hea dɔléle mawo vɛ.

Nukae nye ŋutinya sia si ku ɖe Gram-positive kple Gram-negative ŋu?

Esiae nye Gram ƒe ɖiƒoƒo dodokpɔa ƒe akpa vevitɔ kekeake. Bu dɔlékuiwo be wonye nugbagbevi suesuesue siwo do awu ƒomevi vovovowo. Gram ƒe ɖiƒoƒo dodokpɔa bia be woatsɔ ɖi tɔxɛ ƒomevi geɖe ade dɔlékuiawo ŋu. Emegbe le vovototo siwo le dɔlékuiawo ƒe lãmenugbagbeviwo ƒe gli ŋu nu la, womana ɖe amadede eve me.

1. Gram-positive bacteria: Woƒe lãmenugbagbeviwo ƒe gliwo nye nu ɣi aɖe si woyɔna be ``Peptidoglycan.'' Esia tae wotrɔna zua amadede dzĩ-blɔ ne woƒo ɖi wo ɖo.

2. Dɔlékui siwo nye Gram-negative: Lãmenugbagbeviwo ƒe gliwo le sue, eyata woƒe amadede trɔna zua pink-dzẽ ne woƒo ɖi wo.

Azɔ mina míalé ŋku ɖe dɔlékui ƒomevi vevi siwo le hatsotso eve siawo me kple dɔléle siwo wohena vɛ ŋu.

Vovototo kae le Gram ƒe ɖiƒoƒo kple dɔlékuiwo ƒe dekɔnu dome?

Zi geɖe la, wò ɖɔkta ate ŋu aɖo aɖaŋu na wò be nàwɔ dodokpɔ siawo evea siaa ɖekae, gake nyatakaka siwo wonana la to vovo.

• Gram Stain: Esia le abe foto si woɖe enumake ene. Enaa nukpɔsusu si bɔ kaba nenye be dɔlékuiwo le afima, ne ele nenema la, nenye be wonye Gram-positive alo Gram-negative, kple alesi woƒe nɔnɔme le (wole goglo alo abe atikplɔ ene).
• Dɔlékuiwo ƒe Dekɔnu: Esia bia be nàdzi dɔlékui siwo le wò kpɔɖeŋua me ɖe nu tɔxɛ aɖe me le dodokpɔxɔ me. Ele abe numiemie aɖe tsitsi ene. Esia ate ŋu axɔ ŋkeke ʋɛ aɖewo. Ne dɔlékuiawo tsi nyuie ko la, àte ŋu ade dzesi wo ahanya dɔlékuiwutike siwo wɔa dɔ nyuie na wo.

Kpuie ko la, Gram stain naa mɔfiame kaba, evɔ dɔlékuiwo ƒe dekɔnu naa kpeɖodzi si ŋu kakaɖedzi le wu .

Aleke wowɔa Gram Stain dodokpɔae?

Woate ŋu ama ɖoɖo sia ɖe afɔɖeɖe vevi etɔ̃ me.

1. Kpɔɖeŋua nuƒoƒoƒu

Mɔnu si dzi woato axɔ kpɔɖeŋua ato vovo le afisi nèsusu be dɔlélea le ye ŋu nu.

• Ne wozã atike si wotsɔna ƒoa ɖae: Woate ŋu atsɔ kotoku aɖe axɔe tso teƒewo abe ŋɔti, ŋɔti, ŋutigbalẽ ƒe abi, kple vidzinuwo ƒe teƒewo ene.
• Nusiwo dona le lãme na ame nuƒoƒoƒu: Àte ŋu aƒo nusiwo dona le lãme na ame abe ʋuʋudedi, aɖuɖɔ, kple aɖuɖɔ ene nu ƒu ɖe nugo dzadzɛ aɖe me.
• Fine-needle aspiration: Wozãa mɔnu sia tsɔ xɔa tsi tso ŋutilãa me, le kpɔɖeŋu me, tso ƒunukpeƒewo, ƒo xlã lãkusiwo, alo ƒo xlã dzia.

2. Nusi dzɔna le dodokpɔxɔa me

Ne wò kpɔɖeŋua va ɖo dodokpɔxɔa me la, atikewɔƒe ƒe dzɔdzɔmeŋutinunyala aɖe akakae ɖe ahuhɔ̃e si wotsɔna ƒua gbe dzi vie. Emegbe, woatsɔ amadede tɔxɛ ene, siwo ŋu míeƒo nu tsoe va yi, akpe ɖe eŋu ahaklɔe.

3. Nusuekpɔmɔ̃ dzi kpɔkpɔ

Mlɔeba la, wotsɔa nusuekpɔmɔ̃ léa ŋku ɖe slide sia si wodzra ɖo la ŋu. Esia nye ne míelé ŋku ɖe nenye be dɔlékuiwo le wo me, woƒe amadede (aɖabɛ/blɔ alo pink/dzẽ), woƒe nɔnɔme (goglo (cocci) alo atikplɔ ƒe nɔnɔme (bacilli)), kple alesi woƒo wo nu ƒu ɖekae (wo ɖeɖe, eve eve, alo ƒuƒoƒo).

Nukae numekuku ŋuti nyatakakaa gblɔ?

Ðewohĩ nu vevi eve ado tso eme le wò dodokpɔ ŋuti nyatakakaa me.

• Negative Gram stain (Womekpɔ nugbagbe aɖeke o): Esia fia be womekpɔ dɔlékui aɖeke le slide la dzi o. Gake ɣeaɖewoɣi la, esia ate ŋu adzɔ ne dɔlékui ʋɛ aɖewo koe le eme. Eyata ɖewohĩ ahiã be nàlala dekɔnuwo ŋuti nyatakakaa.
• Positive Gram stain: Esia fia be dɔlékuiwo nɔ wò kpɔɖeŋua me. Nyatakakaa ate ŋu anye nyatakaka tsitotsito abe:
• Dɔlékui ƒomevi: Gram-positive alo Gram-negative?
• Dɔlékuiwo ƒe nɔnɔme: `cocci` (goglo) alo `bacilli` (atiwo)?
• Lãmenugbagbevi bubuwo: Woate ŋu ade dzesii hã ne lãmenugbagbevi bubuwo le eme, abe ʋumenugbagbevi ɣiwo (si nye dɔlékui ƒe dzesi ene).

Ne ɖɔktaa ƒo nyatakaka siawo katã nu ƒu hetsɔ wo sɔ kple wò dzesiwo la, adze atikewɔwɔ nyuitɔ kekeake gɔme na wò.