Mina míasrɔ̃ nu tso dɔlélenutsiŋutete ƒe dodokpɔ si kpena ɖe ame ŋu wòdea dzesi kansa lãmenugbagbeviwo ŋu ko.

Ðewohĩ mèse nya `(Immunophenotyping)` ŋkɔ kpɔ o. Gake esia nye dodokpɔ vevi aɖe ŋutɔ, vevietɔ le dɔléle aɖewo didi me, le kpɔɖeŋu me, ʋumenugbagbeviɣidɔ, ʋumenugbagbeviɣidɔ, kple kansa bubuwo. Eyata, egbea míaƒo nu tso dodokpɔ sia ŋu le mɔ bɔbɔe aɖe nu, ale be nàte ŋu ase egɔme nyuie.

Nukae nye Dɔlélenutsiŋutete ƒe Nɔnɔme? Mina míase egɔme bɔbɔe.

Enyo, azɔ mina míakpɔ nusi nya sia `(Immunophenotyping)` fia ɖa. Togbɔ be esia ɖi dzɔdzɔmeŋutinunya me nya aɖe vie hã la, le nyateƒe me la, ŋutinyaa le bɔbɔe ŋutɔ.

Nya ‘Dɔlélenutsiŋutete’ fia nane si do ƒome kple míaƒe dɔlélenutsiŋutete . Le dodokpɔ sia me la, dɔléleŋutinunyala (ɖɔkta si bi ɖe dɔlélewo didi kple wo dada me) zãa protein ƒomevi bubu si woyɔna be ‘antibodies’ si tso mía ŋutɔwo ƒe dɔlélenutsiŋutete me tsɔ dia protein tɔxɛ siwo woyɔna be ‘antigens’ le míaƒe lãmenugbagbeviwo me. Bu ‘dɔlékuiwutike’ siawo be wonye safui, eye bu ‘dɔlékuiwutikewo’ be wonye gaƒoɖokui. Abe gaƒoɖokui si sɔ na safui ɖesiaɖe ene la, ‘dɔlékuiwutike’ aɖe koŋ abla ɖe ‘dɔlékuiwutike’ aɖe koŋ ŋu. Ne míagblɔe bubui la, eve ƒomevi aɖe si sɔ kple wo nɔewo le wo ame evea dome.

Nya ‘phenotype’ fia nɔnɔme siwo woate ŋu akpɔ le gota . Eyata, ne `(antigen)` ma bla ɖe `(antigen)` si sɔ ŋu la, dɔléleŋutinunyala la nya nenye be `(antigen)` ma le lãmenugbagbevi ma si alo mele esi o le dzesi siwo wòɖena fiana nu.

Kpuie ko la, `(Immunophenotyping)` dodokpɔ sia léa ŋku ɖe `(antigens)` dzesi siawo siwo le míaƒe lãmenugbagbeviwo ƒe anyime ŋu eye wòkpɔnɛ nenye be lãmenugbagbevi mawo nye lãmenugbagbevi siwo le lãmesẽ me alo esiwo ŋu kansa le. Azɔ hã, `(antigens)` siawo ateŋu agblɔ kansa lãmenugbagbevi ƒomevi si wònye.

Ɣekaɣie wowɔa dodokpɔ sia (Immunophenotyping)?

Nɔnɔme geɖe li siwo me wò ɖɔkta ate ŋu akafu dodokpɔ sia le.

Tsɔe be xɔ̃wò aɖe, ne míagblɔe be Nimal, le ɖeɖi te eŋu ŋutɔ eye kuvia le eŋu ŋkeke ʋɛ aɖewoe nye esia. Esi ɖɔkta la wɔ ʋudodokpɔ (si nye ``Ʋu Xexlẽme Blibo'', alo ``CBC''), ekpɔe be yeƒe ʋumenugbagbevi ɣiwo ƒe xexlẽme sɔ gbɔ le mɔ si mesɔ o nu. Ekema ɖɔktaa ate ŋu asusui be esia ate ŋu anye ʋumekansa. Ɣemaɣie wòaɖo aɖaŋu be woawɔ ``Immunophenotyping'' dodokpɔ sia be nànya nu geɖe tso nusi tututu le edzi yim ŋu.

Ema fia be:

• Ne ʋu ƒe xexlẽme blibo (`(CBC)`) ƒe emetsonuwo ɖee fia be wò ʋumenugbagbevi ɣiwo ƒe xexlẽme sɔ gbɔ le mɔ si mesɔ o nu.
• Ne ɖikeke aɖe li be ʋumekansa ƒomevi aɖewo le eŋu, le kpɔɖeŋu me ʋumenugbagbeviɣidɔ alo ʋumenugbagbeviɣidɔ la, esia ate ŋu akpe ɖe ame ŋu wòate ɖe dɔléle si wokpɔ ŋu vie.

Gakpe ɖe eŋu la, numekulawo le mɔnu sia si nye ``Immunophenotyping'' hã zãm tsɔ le nu srɔ̃m tso alesi kansa lãmenugbagbeviwo wɔa nu ɖe ​​``antigen'' dzesi tɔxɛwo ŋu, vevietɔ ``immunotherapy'' ŋu.

Aleke dodokpɔ sia wɔa dɔe?

`(Immunophenotyping)` dodokpɔ sia ƒe akpa gãtɔ dzɔna le dodokpɔxɔ me, ne woxɔ lãkusi alo tsi ƒe kpɔɖeŋu le asiwò vɔ. Ne míagblɔe tututu la, nusi nàwɔ koe nye be nàtsɔ kpɔɖeŋua ana, eye eŋutinunyala siwo le dodokpɔxɔa me awɔ susɔea.

Ðɔkta akpa gãtɔ zãa dodokpɔxɔ ƒe mɔnu aɖe si woyɔna be ``Flow Cytometry'' tsɔ wɔa dodokpɔ sia. Gakpe ɖe eŋu la, ɣeaɖewoɣi la, wozãa mɔnu aɖe si woyɔna be ``Immunohistochemistry - IHC'' hã. Na míalé ŋku ɖe nusiawo evea siaa ŋu.

Mɔnu vevi eve li...

(Flow Cytometry) mɔnu (mɔnu si wozãna wu) .

Ne èzã mɔnu sia la, àte ŋu alé ŋku ɖe ʋu gã alo ʋumenugbagbevi siwo woɖe tso lãkusiwo me ŋu. Ale si wòwɔa dɔe nye esi:

• Kpɔɖeŋua nuƒoƒoƒu: Zi geɖe la, esia bia be woaɖe ƒumeŋuɖui ƒe kpɔɖeŋu tso ƒumeŋuɖui ƒe lãmenugbagbeviwo me dzodzro me, alo ʋu sue aɖe. Ɣeaɖewoɣi la, woate ŋu axɔ kpɔɖeŋua tso aklã me tsi alo tsi si le ƒodo me.
• Kpɔɖeŋua takpɔkpɔ: Nudokpɔƒe dɔwɔlawo dzraa kpɔɖeŋua ɖo hena ``Immunophenotyping'' dodokpɔ. Wotsɔa nu aɖewo kpena ɖe eŋu be lãmenugbagbeviawo nagaku o.
• Tsɔtsɔ kpe ɖe (Antibody): Eyome, wotsɔa ``antibody`` siwo wowɔ be woatsɔ abla ɖe ``antigen`` si wodi be yewoake ɖo la kpena ɖe eŋu. Wotsɔa ``dɔlékuiwutike`` siawo kpena ɖe ``fluorescent compound`` alo ``immunofluorescence marker`` ŋu. Le dzesi sia ta la, ne ``antibody`` bla ɖe ``antigen`` ŋu la, lãmenugbagbeviawo doa kekeli.
• Dɔwɔwɔ (Flow Cytometry): Fifia wotsɔa kpɔɖeŋu si wodzra ɖo kple `(antibodies)` mawo dea mɔ̃ aɖe si woyɔna be `(Flow Cytometer)` me. Mɔ̃ sia ƒe sensor tɔxɛwo (`detectors`), siwo ateŋu ade dzesi kekeli si tso `(immunofluorescence marker)` ma me. Eyata, `(Flow Cytometer)` sia dzidzea lãmenugbagbevi agbɔsɔsɔme si le afima siwo bla `(antigen-antibody)` (i.e. emit light).

(Immunohistochemistry - IHC) ƒe mɔnu

Dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe dɔwɔwɔ nye mɔnu si ate ŋu ade dzesi dɔlékuiwutike tɔxɛ aɖewo ƒe anyinɔnɔ le lãmenugbagbeviwo ƒe kpɔɖeŋu me. Afɔɖeɖe siwo wowɔna le dodokpɔxɔa me sɔ kple esiwo wowɔna le flow cytometry me ŋutɔ. Vovototo vevitɔe nye be le IHC me la, wotsɔa enzyme aɖe dea dɔlékuiwutikeawo me ɖe atike si me keklẽŋusẽ le teƒe. Enzyme sia tsɔa amadede aɖe koŋ ƒoa ɖi lãmenugbagbeviwo ne antigen la le wo me. Ne dɔléleŋutinunyala kpɔ ɖi aɖe le nusuekpɔmɔ̃ me la, efia be dɔlékuiwutikea le lãmenugbagbeviawo me.

Nukae nye dodokpɔ sia (Immunophenotyping) ƒe seɖoƒe?

Nusi gblẽ le eŋu wue nye be menye dodokpɔxɔ ɖesiaɖe si wɔa dodokpɔ sia si ƒe `` ɖoɖowɔɖi deŋgɔwo sɔ .'' Eyata ɣeaɖewoɣi la, tɔtrɔ suesuesuewo ate ŋu anɔ emetsonuwo me.

Gake nu ɖeka aɖe si dzi wòle be nàɖo ŋkui. Ðɔktawo menɔa te ɖe `(Immunophenotyping)` ƒe emetsonu siawo ɖeɖeko dzi dea dzesi dɔléle aɖe o. Wotsɔe sɔ kple nu bubu geɖewo, abe wò dzesiwo kple dodokpɔ bubuwo me tsonu ene, be woaƒo nya ta. Esia fia be dodokpɔ sia ɖeɖe mefia be kansa le ŋuwò o. Enye nɔnɔmetata si lolo wu ƒe akpa ɖeka ko, dzɔtsoƒe ɖeka koe wònye si ate ŋu akpe ɖe ŋuwò nàsrɔ̃ nu geɖe tso wò lãmesẽ ŋu.

Aleke emetsonuwo le? Nukae wogblɔna na mí?

Wò ɖɔkta anɔ te ɖe dɔléleŋutinunyala ƒe numekuku dzi aɖe nusi wò emetsonuwo fia me na wò. ``Immunophenotyping'' dodokpɔ ƒe emetsonuwo menye ``nyui'' alo ``vɔ̃'' ko o. Ebia dɔléleŋutinunyala si wona hehee be wòase ``nyatakaka'' siwo ``Flow Cytometer'' wɔ tso kpɔɖeŋua ŋu gɔme eye wòaɖe nusi wògblɔ gɔme nyuie.

Edzroa nuwo abe:

• Nukae nye `ʋumenugbagbevi ɣiwo ƒe ƒomevi`?
• Tsitsi ƒe afɔɖeɖe ka mee wole?
• Lãmenugbagbevi nenie le antigen si dim nèle la me?

Dɔléleŋutinunyala la tsɔa nyatakaka siwo tso wò kpɔɖeŋua me sɔna kple nyatakaka siwo tso ame siwo le lãmesẽ me siwo ŋu kansa mele o gbɔ, kpakple nyatakaka siwo tso amesiwo ŋu ʋumenugbagbeviɣidɔ alo ʋumenugbagbeviɣidɔ ƒomevi aɖe koŋ le gbɔ. Ezãa nɔnɔme siawo kple dodokpɔ bubuwo me tsonu tsɔ nyaa alesi gbegbe wòanya wɔ be kansa si ŋu nya ku ɖo la nadze dziwò.

Ɣekaɣie wòle be maƒo ka na nye ɖɔkta?

Ne nyabiase alo dzimaɖitsitsi aɖe le asiwò ku ɖe wò dɔwɔwɔ me tsonu alo nusi wofia ŋu la, kpɔ egbɔ be yeƒo ka na yeƒe ɖɔkta . Dɔlékuiwutikewo ƒe dɔwɔwɔ nye dodokpɔ sesẽ aɖe si wowɔna le dodokpɔxɔ me si léa ŋku ɖe lãmenugbagbeviwo ƒe nɔnɔmewo ŋu. Gake wò ɖɔkta ate ŋu aɖe alesi wòkpɔa ŋusẽ ɖe wò lãmesẽ dzii me le nya bɔbɔewo me.

Ðewohĩ mèse veve aɖeke o, eye ewɔ nuku na wò be nèkpɔe be wò ʋumenugbagbevi ɣiwo sɔ gbɔ le ʋudodokpɔ si wowɔna edziedzi (``CBC'') me. Alo ɖewohĩ ɣeyiɣi aɖee nye esia wò lãme mesẽ o. Ke hã, ``CBC'' ƒe emetsonu mawo nye afɔɖeɖe gbãtɔ ko le nusi le edzi yim la nyanya me, eyata ahiã be nàgbɔ dzi ɖi.

Nyatakaka vevi geɖe le wò lãmenugbagbeviwo me ku ɖe wò lãmesẽ ŋu. Dɔlélenutsiŋutete ƒe nɔnɔme nye dodokpɔ si léa ŋku ɖe wò lãmenugbagbeviwo ŋu eye wògblɔa wò lãmesẽ ŋu nya na wò. Le ɣeyiɣi siawo tɔgbe me la, emetsonuwo lala ate ŋu ado vɔvɔ̃ na ame ŋutɔŋutɔ eye wòana ame natsi dzimaɖi. Ne èhiã seselelãme ƒe kpekpeɖeŋu la, ƒo nu tso eŋu na wò ɖɔkta. Azɔ hã, ne nya aɖe le asiwò ku ɖe dodokpɔa me tsonu ŋu la, mègahe ɖe megbe le biabia me o.

Nu vevitɔ kekeake siwo wòle be míasrɔ̃ tso esia me

Enyo, eyata mina míaƒo nu tso nu vevitɔ kekeake siwo dzi míaɖo ŋkui le `(Immunophenotyping)` dodokpɔ sia si ŋu míeƒo nu tsoe egbea ŋu kpuie.

• Esia nye dodokpɔ tɔxɛ aɖe si wowɔna le dodokpɔxɔ me. Ekpena ɖe ame ŋu koŋ le kansa abe ʋumenugbagbeviɣidɔ kple ʋumenugbagbeviɣidɔ ene didi me.
• Wowɔa esia to protein tɔxɛ siwo woyɔna be ``antigens'' siwo le míaƒe lãmenugbagbeviwo ƒe anyime la didi me.
• Mɔnu eve siwo wozãna wu enye ``Flow cytometry'' kple ``Immunohistochemistry - IHC''.
• Nusiwo dona tso eme la sesẽ vie, eyata dɔléleŋutinunyala aɖe dzroa wo me.
• Esia nenɔ susu me na wò: Ame aɖeke mate ŋu agblɔ na wò be kansa le ŋuwò ne èlé ŋku ɖe dodokpɔ sia me tsonu ŋu ko o. Enye dɔlélewo didi ƒe ɖoɖoa ƒe akpa ɖeka ko.
• Ne nya aɖe le asiwò ku ɖe emetsonuwo ŋu la, mègavɔ̃ be yeabia wò ɖɔkta o. Wole afima be yewoakpe ɖe ŋuwò.

` Dɔlélenutsiŋutete ƒe nɔnɔme, kansa, ʋumenugbagbeviɣidɔ, ʋumenugbagbeviɣidɔ, ʋudodokpɔ, lãmenugbagbeviwo, dɔlékuiwutikewo