Ðewohĩ wò ɖɔkta ɖe gbe be woawɔ dodokpɔ tɔxɛ aɖe atsɔ aɖo kpe dɔlélea dzi. Èse nya "immunostaining" ŋu nya kpɔa? Ðewohĩ aɖi nya sesẽ vie eye wòasesẽ. Gake le nyateƒe me la, enye dodokpɔ si de ŋgɔ ŋutɔ le nudokpɔƒe si kpena ɖe mía ŋu míekpɔa dɔlélewo abe kansa kple dɔlékui vovovowo ene nyuie. Egbea, míaƒo nu tso eŋu le mɔ bɔbɔe aɖe ŋutɔ nu.
Kpuie ko la, nukae nye Immunostaining?
Dɔlélenutsiŋutete ƒe amadede nye mɔnu tɔxɛ aɖe si wozãna tsɔ dea dzesi protein molecule aɖe koŋ si ɖɔkta di be yeadi le protein gbogbo siwo le wò ʋu, lãkusi, alo ƒumeŋuɖui ƒe kpɔɖeŋu me dome.
Tsɔe be èle agbalẽ aɖe xlẽm. Èzãa nuŋlɔti si dea dzesi nyagbewo tsɔ tea gbe ɖe nyagbe vevitɔwo kekeake dzi le agbalẽa me ale be nàte ŋu akpɔ wo bɔbɔe. Nu mae mɔnu sia wɔna. Le protein akpe geɖe siwo le míaƒe ŋutilã ƒe lãmenugbagbeviwo me dome la, mɔnu sia kpena ɖe mía ŋu míedea dzesi protein tɔxɛ aɖewo (míeyɔa antigen siawo ) siwo do ƒome kple dɔléle aɖe to “amadede wo me” me.
Wozãa nu tɔxɛ siwo woyɔna be dɔlékuiwutikewo tsɔ wɔa "amadede" sia . Ne míagblɔe tututu la, wozãa dɔlékuiwutike si ate ŋu abla ɖe dɔlékuiwutike aɖe koŋ ŋu. Wotsɔa dɔlékuiwutike sia kpena ɖe amadede tɔxɛ aɖe, enzyme, fluorescent molecule, alo sika ƒe akpa aɖewo ŋu. Eyata ne dɔlékuiwutike ma bla ɖe dɔlékuiwutike si ŋu nya ku ɖo ŋu la, ne míelé ŋku ɖe eŋu le nusuekpɔmɔ̃ me la, etrɔna zua amadede dzeani aɖe eye míete ŋu kpɔnɛ nyuie.
Nu vevitɔe nye be dɔléleŋutinunyalawoe wɔa dodokpɔ sia le nudokpɔƒe. Togbɔ be woxɔa ʋu aɖe (abe ʋu alo lãkusi ene) le asiwò le kɔdzi hã la, wowɔa dodokpɔa le dodokpɔxɔ me.
Nukawoe nye dɔlélenutsiŋutete ƒe dodokpɔ ƒomevi veviwo?
Mɔnu vovovowo li wozãna tsɔ wɔa dodokpɔ siae. Wò ɖɔkta atia mɔnu si sɔ wu le wò atikewɔwɔ ƒe nɔnɔme nu. Na míalé ŋku ɖe mɔnu vevi aɖewo ŋu.
| Dodokpɔmɔnu | Nukae esia wɔna eye nukae wozãnɛ na? |
|---|---|
| Dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe dɔwɔwɔ | Esiae nye mɔnu si wozãna wu. Le afisia la, wotsɔa lãkusi aɖe si woɖe le asiwò la dana ɖe glass slide dzi, wotsɔa dɔlékuiwutike si sɔ kpena ɖe eŋu, eye wozãa amadede tɔxɛ aɖe tsɔ dea amae. Emegbe wotsɔa nusuekpɔmɔ̃ léa ŋku ɖe eŋu. Esia kpena ɖe ame ŋu ŋutɔ le kansa ƒomevi vovovowo nyanya me , ahɔhɔ̃mekawo ƒe dɔlélewo, kple ʋumenugbagbeviɣiwo ƒe hatsotsowo me toto me. |
| Sisi ƒe lãmenugbagbeviwo ƒe dodokpɔ | Bu eŋu abe nuɖuɖu siwo wodzrana le fiase gã aɖe me ƒe scan wɔwɔ ene. Le mɔnu sia me la, wozãa dɔlékuiwutike siwo le amadede vovovowo me tsɔ toa laser-ya aɖe me toa wò lãmenugbagbeviwo me ɖekaɖeka. Emegbe mɔ̃a ate ŋu axlẽ lãmenugbagbeviawo, aɖo wo ɖe ɖoɖo nu, ahama wo dome. Wozãa esia tsɔ dea dzesi kansawo abe ʋumenugbagbeviɣidɔ kple ʋumenugbagbeviɣidɔ, dɔléle vovovo siwo xɔa ame, kple dɔléle siwo dɔlélenutsiŋutete wɔna . |
| Dɔlélenutsiŋutete ƒe elektrɔnik nusuekpɔmɔ̃ (EM dɔlélenutsiŋutete ƒe dzeside) . | Esia wɔnɛ be woate ŋu akpɔ kpɔɖeŋu aɖe le lolome gã ŋutɔ me, le mɔ si me kɔ ŋutɔ nu. Le afisia la, sika ƒe akpa siwo le abe colloidal ene la léna ɖe dɔlékuiwutikewo ŋu. Ne wotsɔ elektrɔnik nusuekpɔmɔ̃ kpɔ sika siawo nyuie abe dometsotso yibɔwo ene. Eyata ne dɔlékuiwutike aɖe nya lé ɖe dɔlékuiwutike aɖe ŋu ko la, woate ŋu ade dzesii bɔbɔe. Wozãa mɔnu sia hã tsɔ dea dzesi dɔlékuiwo ƒe dɔlékuiwo eye wokpɔa afisi dɔlékui ƒe dɔlékuiwutikewo le le atikewɔmɔnuwo me. |
Aleke wòle be madzra ɖo ɖe dodokpɔ sia ŋu?
Zi geɖe la, mehiã be nàdzra nu tɔxɛ aɖeke ɖo na dɔlélenutsiŋutete ƒe dodokpɔ o.
Gake hafi nàxɔ ʋu aɖe (i.e., axɔ ʋu alo lãkusi aɖe, abe ŋutilã ƒe akpa aɖe ene), wò ɖɔkta ana mɔfiame siwo me kɔ wò ku ɖe nusi nàwɔ ŋu. Le kpɔɖeŋu me, ne wole ŋutilã ƒe akpa aɖe ɖeɖem na wò la, woate ŋu agblɔ na wò be mègaɖu nu alo ano naneke hafi woawɔ dɔ na wò o. Ele vevie be nàwɔ ɖe mɔfiame mawo dzi pɛpɛpɛ.
Ɣeyiɣi didi kae dodokpɔa xɔa? Ɣekaɣie woakpɔ emetsonuwo?
Ɣeyiɣi si wòxɔna hafi esia dzɔna la toa vovo le mɔnu si wozã nu.
- Mɔnu aɖewo ate ŋu ahe emetsonuwo vɛ le gaƒoƒo ʋɛ aɖewo ko me .
- Mɔnu bubu aɖewo ate ŋu axɔ ŋkeke 10 .
Gbesigbe woaɖe wò kpɔɖeŋua la, ɖɔkta alo dɔwɔlawo dometɔ aɖe ana nàkpɔ ɣeyiɣi didi si wòaxɔ hafi woakpɔ emetsonuwo, eyata mègatsi dzi ɖe ema ŋu o.
Nukae emetsonuwo gblɔ?
Dɔléleŋutinunyala si le nudokpɔƒea alé ŋku ɖe wò kpɔɖeŋua ŋu eye wòaɖo nusiwo ŋu woke ɖo ŋuti nyatakaka bliboa ɖe ɖɔkta si le dɔ wɔm na wò. Emegbe wò ɖɔkta atsɔ nusiwo ŋu woke ɖo le nyatakakaa me asɔ kple wò nɔnɔmea eye wòaɖe nusi wòfia me na wò le nya bɔbɔewo me.
Nukae adzɔ emegbe ne emetsonuwo mesɔ o?
Ne dodokpɔa ɖo kpe atikewɔwɔ ƒe nɔnɔme aɖe dzi la, mègatsi dzodzodzoe o. Wò ɖɔkta aɖe emetsonuwo me na wò bliboe eye wòaƒo nu tso wò atikewɔwɔ ƒe tiatiawɔblɔɖewo ŋu.
Le go aɖewo me la, atikewɔlawo ƒe ƒuƒoƒo aɖe si tso dɔ vovovowo me ate ŋu aƒo ƒu adzro atikewɔwɔ ƒe ɖoɖo nyuitɔ kekeake me. Amesiame ƒe taɖodzinue nye be yeana atike nyuitɔ kekeake wò. Eyata ƒo nu kple wò ɖɔkta tso nyabiase alo nu siwo ŋu nètsi dzi ɖo ŋu.
Gbedasi si Wotsɔ Yi Aƒeme
- Dɔlélenutsiŋutete ƒe amadede nye dodokpɔ tɔxɛ aɖe si wowɔna le nudokpɔƒe si zãa dɔlékuiwutikewo tsɔ dea dzesi dɔlélewo abe kansa kple dɔlékuiwo ene pɛpɛpɛ.
- Dodokpɔ sia ƒomevi vovovowo li, eye wò ɖɔkta atia mɔnu si sɔ wu le wò atikewɔwɔ ƒe nɔnɔme nu.
- Wò ɖɔkta ana nànya mɔfiame siwo dzi nàwɔ ɖo hafi atsɔ kpɔɖeŋu aɖe ana wò be woadoe kpɔ.
- Ɣeyiɣi si wòxɔna hafi woate ŋu axɔ dodokpɔa me tsonu ate ŋu ato vovo, eye ne wonya kpɔ dodokpɔa me tsonu ko la, wò ɖɔkta aɖe wo me na wò ahadzro afɔɖeɖe siwo kplɔe ɖo me, ne ehiã.
- Dzro nyabiase ɖesiaɖe si le asiwò ku ɖe wò dodokpɔ me tsonu alo wò atikewɔwɔ me ŋu na wò ɖɔkta gaglãa.
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