ELISA ye mun ye? An ka nin laboratuwari sɛgɛsɛgɛli in dɔn cogo nɔgɔman na!

N’a sɔrɔ aw ye ‘ELISA’ daɲɛ mɛn dɔgɔtɔrɔ dɔ fɛ walima laboratuwari ka kunnafoni dɔ fɛ , ɔnhɔn? Walima n’a sɔrɔ o ye daɲɛ kura ye. O la, ELISA ye mun ye, a bɛ mun ɲini, ani a bɛ baara kɛ cogo di? An ka kuma o kan bi dɔrɔn, i n’a fɔ i bɛ kuma i teri dɔ fɛ. O kɔ, i bɛ se ka faamuyali ɲuman sɔrɔ o ko la.

ELISA ye mun ye? A ka nɔgɔn kosɛbɛ!

N’an y’a fɔ cogo nɔgɔman na, ELISA ye fɛɛrɛ kɛrɛnkɛrɛnnen ye min bɛ kɛ laboratuwariw kɔnɔ. A bɛ baara kɛ i n’a fɔ detekti. A bɛ fɛn dɔw ɲini an farikolo la, i n’a fɔ farikolo tangalanw, farikolo tangalanw , farikolojɔlifɛnw, ani ɔrimɔni , ka u hakɛ jateminɛ.

Miiri k’a filɛ, nin sɛgɛsɛgɛli in bɛ kɛ ni aw joli, joli, sugunɛ, daji walima kunsɛmɛji (CSF) ta ye. Daɲɛ minnu ye ‘ELISA’ ye, olu kɔrɔ ye ‘enzyme-linked immunosorbent assay’ ye. A bɛ wele tuma dɔw la ko EIA sɛgɛsɛgɛli.

ɲininikɛlaw bɛ nin ELISA wele ko farikolo tangacogo sɛgɛsɛgɛli sanu . O kɔrɔ ye ko a ye fɛɛrɛ ye min bɛ se ka da a kan kosɛbɛ ani min bɛ tiɲɛ. Sɛgɛsɛgɛli minnu bɛ kɛ ni nin ELISA fɛɛrɛ in ye , olu bɛ se ka dɛmɛ don ka bana suguya caman sɛgɛsɛgɛ , k’a ta banakisɛw ni banakisɛw fɛ (misali la, Lyme bana, sidabanakisɛ) ka se banaw ma minnu bɛ sɔrɔ ɔrimɔni fɛ i n’a fɔ sɔgɔsɔgɔninjɛ

Yala aw b’a dɔn ko hali kɔnɔmaya sɛgɛsɛgɛliw bɛ kɛ so kɔnɔ, olu sinsinnen bɛ nin ELISA fɛɛrɛ in kan wa? U bɛ ɔrimɔni dɔ sɔrɔli ɲini min bɛ wele ko human chorionic gonadotrophin (HCG) , n’a bɛ fɔ fana ko ‘kɔnɔmaya hormone’.

O la, nin farikolo tangalanw ye mun ye?

O bɛ ɲɛ, an k’a lajɛ sisan farikolo tangalanw ye min ye. Ninnu ye sɛgɛsɛgɛliw ye minnu bɛ tali kɛ banakisɛfagalanw ni farikolo tangalanw ka jɛɲɔgɔnya la laboratuwari cogoyaw la (o kɔrɔ ye ko laboratuwari kɔnɔ, aw farikolo kɔnɔ tɛ).

Walasa ka o faamuya, a nafa ka bon ka a faamu fɔlɔ farikolo tangalanw ni banakisɛfagalanw bɛ baara kɛ cogo min na aw fari la. farikolo tangalanw ye fɛnw ye minnu bɛ aw farikolo tangacogo kɛ . U bɛ siri fɛnw na minnu man ɲi aw farikolo ma, ka a dɛmɛ ka u bɔ a la. U bɛ i n'a fɔ an ka jamana lafasalitɔnw. BanakisɛfagalanwAntijeni ye taamasiɲɛ suguya o suguya ye, farikolo tangalanw bɛ se ka min dɔn. A ka ca a la, o banakisɛfagalanw ye farikolojɔli dumuniw ye walima sukaro minnu bɛ sɔrɔ farikolokisɛw walima banakisɛw sanfɛ.

Antijeni bɛ se ka sɔrɔ fɛn suguya caman sanfɛ , i n’a fɔ:

• Virus (banakisɛfagalan).
• Banakisɛw
• Alɛrijini - misali la, faritanabanakisɛ minnu bɛ dumuni na, faritanabanakisɛ minnu bɛ fiɲɛ na
• Parazitiw (parazites).
• Protéines (banakisɛfagalanw).
• Kansɛri banakisɛw
• Kɔnɔnatumuw minnu bɛ kɛ cogo la min bɛ i farikolo la

O la, farikolo tangalanw sɛgɛsɛgɛli la, dɔnnikɛlaw minnu bɛ laboratuwari la, olu bɛ antijeni walima farikolo tangalanw kɛ u bolo walasa ka a sɛgɛsɛgɛ ni antigène walima farikolo tangalan dɔ bɛ ji la (misali la, joli ta) min bɔra aw la. A sɔrɔla?

Sɛgɛsɛgɛli suguya minnu bɛ kɛ ni ELISA fɛɛrɛ ye, olu bɛ se ka banakisɛfagalan kɛrɛnkɛrɛnnenw ni banakisɛfagalanw sɔrɔli dɔn.

ELISA fɛɛrɛ bɛ kɛ mun na?

Furakɛcogo sɛgɛsɛgɛli suguya caman bɛ kɛ ni ELISA fɛɛrɛ ye. Nka min nafa ka bon kosɛbɛ, o ye ko aw ka laboratuwari ka kunnafoni tɛna ‘ELISA sɛgɛsɛgɛli’ fɔ. O kun ye ko ELISA ye fɛɛrɛ dɔrɔn ye min bɛ kɛ laboratuwari kɔnɔ, wa sɛgɛsɛgɛli jatebaliya bɛ yen minnu bɛ se ka kɛ ni o fɛɛrɛ ye. I n’a fɔ fɛɛrɛ min bɛ kɛ ka dumuni labɛn, aw bɛ se ka dumuni suguya wɛrɛw labɛn ni o fɛɛrɛ ye.

ELISA kɛcogo min ka teli ka kɛ, o ye ka banakisɛfagalanw dɔn ani k’u suman. I n'a fo:

• Banakisɛfagalanw, banakisɛfagalanw walima ɲɛgɛnɛsiralabanaw: Banakisɛfagalanw misaliw ye Lyme bana, brucellosis ani sifili ye. Banakisɛfagalanw misaliw ye sidabanakisɛ ni sugunɛbilenni A, B, ani C. Ɲɛgɛnɛsiraw ka banakisɛfagalan misali dɔ ye funteni banakisɛ ye (kandida).
• Autoanticorps : Olu ye farikolo tangalanw ye minnu bɛ kɛ ka aw yɛrɛ ka farikolokisɛw tiɲɛ. Bana caman minnu bɛ sɔrɔ farikolo tangalanw fɛ, olu bɛ sɔrɔ o farikolo tangalanw fɛ. Misali dɔ ye sukarodunbana suguya fɔlɔ ye. Nin cogo in na, kun dɔnnen dɔ kosɔn, aw farikolo bɛ farikolo tangalanw ci ka taa aw yɛrɛ la minnu bɛ inisɔndiyalanw tiɲɛ aw ka pankreyasi kɔnɔ.

ELISA fɛɛrɛ wɛrɛ minnu bɛ kɛ ka caya, olu ye:

• Ɔrimɔni hakɛ dɔnni ni a jateminɛni:Misali dɔw ye hadamaden ka gonadotropin (HCG), hormone (hormone) min bɛ folikɛlanw lawuli (FSH), ani tetarasikilini.
• Banaw jɛnsɛnni kɔlɔsili sigida la: Misali la, ni klamidi walima kunfilanitu (an b’a wele ko ‘mura’).
• Joli dilen sɛgɛsɛgɛli kɛli banakisɛw yɔrɔw la: misali la, sidabanakisɛ.
• Dɔrɔgu tali dɔnni min tɛ furakɛli ye: Misali la, ka a lajɛ ni anfetamini walima kokayini bɛ aw ka sigida la. Ninnu ye sɛgɛsɛgɛli sɛgɛsɛgɛli dɔrɔn de ye.
• Tumu taamasiɲɛw hakɛ dɔnni ani u jateminɛni : Ninnu ye fɛn o fɛn ye aw fari la min bɛ kunnafoni di kansɛri kan. Misali dɔ ye ɲɛgɛnɛsira kansɛri sɛgɛsɛgɛli ye min bɛ kɛ ka ɲɛgɛnɛsiralabanaw furakɛ.
• Bugun ni dumuni faritanabanaw dɔnni.
• Aw bɛ a lajɛ ni aw boloci kɛra bana dɔw la: misali la, ŋɛɲɛ ni rubɛli.

Nin ELISA fɛɛrɛ in bɛ baara kɛ cogo di? O ye kabako ye dɛ!

O bɛ ɲɛ, sisan an k’a lajɛ nin ELISA sɛgɛsɛgɛli in bɛ baara kɛ cogo min na. Danfara misɛnnin dɔw bɛ yen ka kɛɲɛ ni a bɛ farikolo tangalanw walima banakisɛfagalanw ɲini . An k’a ɲini ka nin faamuya ni misali ta ye farikolo tangalanw ɲinini na. ELISA sɛgɛsɛgɛli fanba bɛ kɛ otomatiki la - o kɔrɔ ye ko baara fanba bɛ kɛ masinw fɛ. Nka walasa ka nin fɛɛrɛ in faamuya, an ka miiri kɔrɔbɔli dɔ la an bɛ min kɛ ni bolo ye.

Miiri k’a filɛ, sidabanakisɛ (adamaden farikolo tangalan banakisɛ) bɛ ka i sɛgɛsɛgɛ ni ELISA fɛɛrɛ ye, ani i ye joli ta kaban. Fɔlɔ, furakɛli laboratuwari dɔnnikɛla walima fɛɛrɛtigi dɔ bɛ sidabanakisɛ yɔrɔ dɔ (o de bɛ kɛ i n’a fɔ banakisɛfagalan) kɛ sɛgɛsɛgɛlikɛlan walima plastiki tabali kan.

O kɔ, dɔnnikɛla bɛ aw joli lajɛ fara sɛgɛsɛgɛlikɛlan walima tasa kan. Ni sidabanakisɛfagalanw bɛ aw joli la, u bɛ siri o banakisɛfagalan na (o la, sidabanakisɛ). Ni sidabanakisɛfagalanw tɛ aw joli la, foyi tɛna kɛ.

O kɔfɛ, dɔnnikɛla bɛ banakisɛfagalan wɛrɛ fara a kan, o banakisɛfagalan filanan in bɛ o sidabanakisɛw ‘dɔn’. O kɔrɔ ye ko nin banakisɛfagalan filanan in bɛ siri sidabanakisɛfagalan fɛn o fɛn na min tun sirilen bɛ banakisɛfagalan na ka kɔrɔ. Nin banakisɛfagalan filanan in bɛ tali kɛ anzimu dɔ la (fɛn min bɛ kemikɛli teliya). O yɔrɔ de la ELISA yɔrɔ ‘min bɛ tali kɛ anzimu la’, o bɛ bɔ yen.

A laban na, dɔnnikɛla bɛ fɛn dɔ fara a kan min bɛ kɛ ni anzimu ye.O kɔrɔ ye ko ni farikolojidɛsɛbanakisɛw bɛ yen, o fɛn in kulɛri bɛ Changé. O kɔrɔ ye ko ni sɛgɛsɛgɛli kɛra ko a ɲɛna, a kulɛri bɛna Changé. Ni farikolo tangalanw tɛ aw bolo o banakisɛfagalan kɛrɛnkɛrɛnnen na (nin ko in na, sidabanakisɛ), a kulɛri tɛ yɛlɛma. O jiralen bɛ iko nɔ jugu.

Min nafa ka bon, o ye ko kulɛri caman cili fanga, o kɔrɔ ye ko kulɛri barika ka bon cogo min na, o bɛ bɛn farikolo tangalan hakɛ ma. O la, ELISA fɛɛrɛ bɛ se ka a dɔn ni banakisɛfagalan bɛ yen ani a hakɛ min bɛ a la. Yala o tɛ kabako ye wa?

ELISA sɛgɛsɛgɛli jaabi ye mun ye?

ELISA sɛgɛsɛgɛli jaabiw bɛ se ka ɲɔgɔn ta ka kɛɲɛ ni fɛn min bɛ sɛgɛsɛgɛ ani laboratuwari min ye sɛgɛsɛgɛli kɛ. ELISA sɛgɛsɛgɛli fanba bɛ jaabi ɲuman walima jaabi jugu di, nka tuma dɔw la, jaabi bɛ se ka kɛ tiɲɛ ye:

• Jateminɛ ɲuman : Jateminɛ ɲuman kɔrɔ ye ko sɛgɛsɛgɛli ye a ɲininen sɔrɔ (misali la, banakisɛfagalan, ɔrimɔni).
• Jateminɛ jugu: Jateminɛ jugu kɔrɔ ye ko sɛgɛsɛgɛli ma fɛn si sɔrɔ min tun bɛ ɲini.
• Jateminɛ tiɲɛnen: Jateminɛ tiɲɛnen kɔrɔ ye ko fɛn dɔ ma ɲɛ sɛgɛsɛgɛli la. O bɛ se ka kɛ gɛlɛya ye misali tacogo la, a tacogo la, walima a sɛgɛsɛgɛli la. Ni o kɛra, aw bɛna a ɲini ka sɛgɛsɛgɛli kɛ tuguni.

Nka, aw ka dɔgɔtɔrɔ bɛna aw ka jaabiw kɔrɔ ɲɛfɔ aw ye.

Yala ne ka kan ka hami ni ne ka jaabiw tɛ cogo la wa?

K’a sababu kɛ aw ka sɛgɛsɛgɛli jaabiw tɛ cogo la, o kɔrɔ tɛ ko bana dɔ bɛ aw la. Tuma dɔw la, fili minnu bɛ kɛ misali tacogo la, u tacogo la, walima u tacogo la, olu bɛ se ka kɛ sababu ye ka jateminɛ kɛcogo juguw lase mɔgɔ ma.

ELISA fɛɛrɛ nafa dɔ ye ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ ko a bɛ se ka kɛ.

• Jateminɛ min bɛ kɛ ni nkalon ye: O bɛ tali kɛ a la ko sɛgɛsɛgɛli jaabi bɛ a jira ko fɛn min kofɔlen don, o bɛ yen, hali ni a tɛ yen tiɲɛ na.
• Jateminɛ min bɛ kɛ ni nkalon ye: O bɛ tali kɛ sɛgɛsɛgɛli jaabi la min b’a jira cogo bɛnbali la ko fɛn min kofɔlen don, o tɛ yen, hali ni a bɛ yen tiɲɛ na.

A ka ca a la dɔgɔtɔrɔw bɛ sɛgɛsɛgɛli wɛrɛw kɛ walasa ka bana dɔ sɛgɛsɛgɛ. ELISA sɛgɛsɛgɛliw bɛ kɛ ka caya i n’a fɔ sɛgɛsɛgɛli sɛgɛsɛgɛliw.

Ni aw ye jaabi dɔ sɔrɔ min tɛ bɛn, aw ka dɔgɔtɔrɔ bɛna kuma aw fɛ o ko la. Aw bɛna a latigɛ ɲɔgɔn fɛ aw bɛna min kɛ o kɔfɛ.

Aw ye aw hakili to a la ko kun o kun bɛ aw la ka ELISA sɛgɛsɛgɛli kɛ, o tɛ bana sɛgɛsɛgɛli laban ye.ELISA sɛgɛsɛgɛli jaabi min tɛ bɛn, o kɔrɔ tɛ ko furakɛli jugumanba dɔ bɛ aw la. Ni aw ye jaabi sɔrɔ min tɛ bɛn, aw ka dɔgɔtɔrɔ bɛna fɛn caman jateminɛ, wa a ka c’a la, a bɛna yamaruya di ka sɛgɛsɛgɛli wɛrɛw kɛ. Aw kana siran ka ɲininkaliw kɛ.

O la sa, cikan jumɛn ka kan ka to an hakili la ka bɔ nin maana in na?

Okey, donc n jigi b’a kan ko sisan aw ye hakilina ɲuman sɔrɔ ELISA kan an ye kuma min kan. N’an y’a fɔ cogo nɔgɔman na:

• ELISA ye laboratuwari sɛgɛsɛgɛli fɛɛrɛ kɛrɛnkɛrɛnnen ye.
• O b’a to an bɛ fɛn caman dɔn an farikolo la, i n’a fɔ farikolo tangalanw, farikolo tangalanw , ani ɔrimɔni .
• Nin fɛɛrɛ in bɛ dɛmɛ ka bana suguya caman dɔn i n’a fɔ kɔnɔmaya, banakisɛw, ɔrimɔni hakɛ, ani faritanabana.
• ELISA sɛgɛsɛgɛli jaabi bɛ se ka kɛ koɲuman ye, ka kɛ ko jugu ye, walima ka kɛ tiɲɛ ye .
• Min nafa ka bon kosɛbɛ: ELISA jaabi tɛ bana sɛgɛsɛgɛli laban ye. Ni aw ye jateminɛ kɛcogo jugu sɔrɔ, aw kana siran, aw bɛ kuma dɔgɔtɔrɔ fɛ, ka sɛgɛsɛgɛli wɛrɛw kɛ ni a mago bɛ a la.

Ni ɲininkali dɔw bɛ aw fɛ, aw kana siran ka dɔgɔtɔrɔ ɲininka. Ale de ye mɔgɔ ɲuman ye min bɛ se k’i dɛmɛ. Aw ka to kɛnɛya la!

` ELISA, joli sɛgɛsɛgɛliw, farikolo tangalanw, banakisɛfagalanw, banaw sɛgɛsɛgɛli, laboratuwari sɛgɛsɛgɛliw