Yu dɔktɔ go dɔn ɔda fɔ mek dɛn du spɛshal tɛst fɔ no if yu gɛt di sik. yu don eva yehri boht di tehm "immunostaining"? I kin tan lɛk se i kɔmplikt smɔl ɛn i nɔ izi. Bɔt fɔ tru, na wan rili advans laboratori tɛst we de ɛp wi fɔ no kɔrɛkt wan sik dɛn lɛk kansa ɛn difrɛn infɛkshɔn dɛn. Tide, wi go tɔk bɔt am insay wan rili simpul we.
Fɔ tɔk am simpul wan, wetin na Immunostaining?
Immunostaining na spɛshal we we dɛn kin yuz fɔ no wan patikyula prɔtin mɔlyul we di dɔktɔ want fɔ luk fɔ bitwin di bɔku bɔku prɔtin dɛn we de na yu blɔd, tisu sɛmpul, ɔ bon mɛrɔ sɛmpul.
Imajin se yu de rid wan buk. Yu kin yuz aylayta pen fɔ aylayt di sɛntɛns dɛn we impɔtant pas ɔl na di buk so dat yu go si dɛn izi wan. Na dat dis we ya de du. pan di tawzin protin dεm we de insay wi bכdi in sεl dεm, dis mεtכd de εp wi fכ no spεshal protin dεm (wi kכl dεn antijen dεm ya ) we riliyt to wan patikyula sik bay we i de "kכla dεm."
dis "staining" dεn de du am bay we dεn de yuz spεshal tin dεm we dεn k כl antibodi . fכ bi prεsis, dεn de yuz wan antibodi we kin כnli biεn to wan spεsifi k antijen. dis antibodi de atak wan spεshal pigmεnt, εnzym, fכrosεnt mכlekyul, כ gold patikyula dεm. So we da antibodi de tay to di antijen we wi de kכl, we wi luk am כnda maykroskכp, i de tכn to fayn fayn kכla εn wi kin si am klia wan.
Di impɔtant tin na dat na patɔlɔjis dɛn we de du dis tɛst na lɛbɔraytri. Ivin if dɛn tek sɛmpul (lɛk blɔd ɔ tisu) frɔm yu na ɔspitul, di tɛst kin apin na di lab.
Wetin na di men kayn tɛst dɛm fɔ immunostaining?
Bɔku we dɛn de we dɛn kin yuz fɔ du dis tɛst. Yu dɔktɔ go pik di we we go fayn fɔ yu bay aw yu de mɛn yu. Lɛ wi luk sɔm pan di men we dɛn.
| Di we aw dɛn de du di tɛst | Wetin dis kin du ɛn wetin dɛn kin yuz am fɔ? |
|---|---|
| Immunohistochemistry we dɛn kin du | Dis na di we aw dɛn kin yuz mɔ. Na ya, dɛn kin put wan tisu sɛmpul we dɛn tek frɔm yu pan glas slayd, ad di rayt antibodi, ɛn yuz spɛshal day fɔ stɛyn am. Dɔn dɛn kin chɛk am ɔnda maykroskɔp. Dis kin rili ɛp fɔ no difrɛn kayn kansa , fɔ klas di nyurolɔjik kɔndishɔn, ɛn blɔd disɔda. |
| Flɔ saytometri fɔ yuz | Tink bɔt am lɛk we yu de skan tin fɔ it na supamakit. Insay dis we, dɛn kin pas yu sɛl dɛn tru wan lɛsa bim, wan bay wan, ɛn dɛn kin yuz antibodi dɛn we gɛt kɔlɔ. Dɔn di mashin kin kɔnt, sɔt, ɛn separet di sɛl dɛn. Dɛn kin yuz dis fɔ no kansa lɛk lukimiya ɛn limfoma, difrɛn sik dɛn we pɔsin kin gɛt, ɛn sik dɛn we pɔsin kin gɛt we i de fɛt insɛf . |
| Imyuno-ilɛktrɔn maykroskɔpi (EM immunolabeling) . | Dis kin mek dɛn ebul fɔ si wan sampul wit rili ay magnifyeshɔn, insay wan we we rili ditayli. na ya, di kolכyd gold patikyula dεm de atak di antibodi dεm. We yu luk dɛn gold ya ɔnda ilɛktron maykroskɔp, yu kin si dɛn gold patikyula dɛn ya klia wan lɛk blak dɔt dɛn. fכ dat, we wan antibodi dכn atak wan antijen, i kin izi fכ no am. dis mכt dεn kin yus bak fכ no di vayral infεkshכn dεm εn fכ fכn di lכkεshכn dεm fכ di vayral antijen dεm na di vaysin dεm. |
Aw a fɔ rɛdi fɔ dis tɛst?
Jɛnɛral wan, yu nɔ nid fɔ pripia ɛnitin we spɛshal fɔ wan immunostaining tɛst.
Bɔt bifo yu tek sɛmpul (i.e., tek blɔd ɔ wan pat pan di tisu, lɛk bayɔpsi), yu dɔktɔ go gi yu klia instrɔkshɔn bɔt wetin fɔ du. Fɔ ɛgzampul, if yu de du bayɔpsi, dɛn kin tɛl yu nɔ fɔ it ɔ drink ɛnitin bifo dɛn du di ɔpreshɔn. I impɔtant fɔ fala dɛn instrɔkshɔn dɛn de di rayt we.
Aw lɔng di tɛst kin tek? Ustɛm di rizɔlt dɛn go de?
Di tɛm we i kin tek fɔ mek dis apin kin difrɛn difrɛn wan bay di we aw dɛn yuz am.
- Sɔm we dɛn kin du tin kin mek pɔsin gɛt rizɔlt insay sɔm awa .
- Sɔm ɔda we dɛn kin tek 10 dez .
Di de we dɛn tek yu sampul, di dɔktɔ ɔ wan pan di wan dɛn we de wok de go gi yu wan rɔf aidia bɔt aw lɔng i go tek fɔ gɛt di rizɔlt, so nɔ wɔri bɔt dat.
Wetin di rizɔlt dɛn se?
Di patɔlɔjis na di lɛbɔraytri go chɛk yu sampul ɛn sɛn wan ful ripɔt bɔt wetin dɛn fɛn to yu dɔktɔ we de trit yu. Dɔn yu dɔktɔ go rilayt wetin dɛn dɔn fɛn na di ripɔt to yu sik ɛn ɛksplen to yu insay simpul wɔd dɛn wetin i min.
Wetin kin apin afta dat if di tin dɛn we kin apin nɔ kin apin?
If di tɛst kɔnfirm se yu gɛt mɛdikal kɔndishɔn, nɔ panik. Yu dɔktɔ go ɛksplen to yu ɔl di tin dɛn we go apin to yu ɛn tɔk bɔt di tritmɛnt dɛn we yu go gɛt.
Sɔntɛnde, wan tim we gɛt dɔktɔ dɛn we kɔmɔt na difrɛn say dɛn kin kam togɛda fɔ tɔk bɔt di bɛst tritmɛnt plan. Ɔlman in gol na fɔ gi yu di bɛst tritmɛnt. So, tɔk to yu dɔktɔ bɔt ɛni kwɛstyɔn ɔ tin we de mɔna yu.
Mɛsej we dɛn kin kɛr go na os
- Immunostaining na spɛshal laboratori tɛst we de yuz antibodi fɔ no kɔrɛkt wan sik dɛn lɛk kansa ɛn infɛkshɔn.
- Difrɛn kayn dɛn de fɔ dis tɛst, ɛn yu dɔktɔ go pik di we we go fayn fɔ yu bay aw yu de mɛn yu.
- Yu dɔktɔ go tɛl yu bɔt di instrɔkshɔn dɛn we yu fɔ fala bifo yu gi yu sampul fɔ tɛst.
- Di tɛm we yu kin tek fɔ gɛt di tɛst rizɔlt kin difrɛn, ɛn we yu dɔn gɛt di rizɔlt, yu dɔktɔ go ɛksplen am to yu ɛn tɔk bɔt di nɛks tin dɛn we yu fɔ du, if nid de.
- Tɔk bɔt ɛni kwɛstyɔn we yu gɛt bɔt yu tɛst rizɔlt ɔr di mɛrɛsin kɔndishɔn opin wan wit yu dɔktɔ.
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