Dokotala wanu mwina adalamula mayeso apadera kuti atsimikizire matenda. Kodi mudamvapo za mawu oti "immunostaining"? Zingamveke ngati zovuta komanso zovuta. Koma kwenikweni ndi mayeso apamwamba kwambiri a labotale omwe amatithandiza kuzindikira matenda molondola monga khansa ndi matenda osiyanasiyana. Lero, tikambirana za izi m'njira yosavuta.
Mwachidule, kodi Immunostaining ndi chiyani?
Kuteteza thupi ku matenda opatsirana ndi njira yapadera yomwe imagwiritsidwa ntchito pozindikira molekyulu inayake ya puloteni yomwe dokotala akufuna kuyang'ana pakati pa mapuloteni ambiri omwe ali m'magazi anu, chitsanzo cha minofu, kapena chitsanzo cha mafuta a m'mafupa.
Tangoganizirani mukuwerenga buku. Mukugwiritsa ntchito cholembera chowunikira kuti mulembe ziganizo zofunika kwambiri m'bukuli kuti muzitha kuziona mosavuta. Ndicho chimene njira iyi imachita. Mwa mapuloteni ambirimbiri omwe ali mkati mwa maselo a thupi lathu, njira iyi imatithandiza kuzindikira mapuloteni enaake (omwe timawatcha ma antigen ) omwe ali okhudzana ndi matenda enaake mwa "kuwapaka utoto."
"Kupaka utoto" kumeneku kumachitika pogwiritsa ntchito zinthu zapadera zotchedwa ma antibodies . Kunena zoona, antibody yomwe ingangolumikizana ndi antigen inayake imagwiritsidwa ntchito. Antibody iyi imalumikizidwa ku pigment yapadera, enzyme, fluorescent molecule, kapena tinthu tagolide. Chifukwa chake antibody imeneyo ikalumikizana ndi antigen yomwe ikukambidwa, tikaiyang'ana pansi pa maikulosikopu, imasintha kukhala mtundu wokongola ndipo timatha kuiwona bwino.
Chofunika kwambiri ndichakuti mayesowa achitike ndi akatswiri a matenda m'labu. Ngakhale kuti mwatenga chitsanzo (monga magazi kapena minofu) kuchipatala, mayesowo amachitikira m'labu.
Kodi mitundu ikuluikulu ya mayeso a chitetezo chamthupi ndi iti?
Pali njira zingapo zomwe zimagwiritsidwa ntchito poyesa izi. Dokotala wanu adzasankha njira yoyenera kwambiri kutengera matenda anu. Tiyeni tiwone njira zina zazikulu.
| Njira yoyesera | Kodi izi zimagwira ntchito yanji ndipo zimagwiritsidwa ntchito pa chiyani? |
|---|---|
| Kuchiza kwa thupi ndi histochemistry | Iyi ndi njira yomwe imagwiritsidwa ntchito kwambiri. Apa, chitsanzo cha minofu yanu chimayikidwa pa galasi, antibody yoyenera imawonjezedwa, ndipo utoto wapadera umagwiritsidwa ntchito kuti uipe. Kenako umawunikidwa pogwiritsa ntchito maikulosikopu. Izi zimathandiza kwambiri kuzindikira mitundu yosiyanasiyana ya khansa , kugawa matenda amitsempha, ndi matenda a magazi. |
| Kuzungulira kwa madzi | Taganizirani izi ngati kusanthula zakudya m'sitolo yayikulu. Mwanjira imeneyi, maselo anu amadutsa mu kuwala kwa laser, limodzi ndi limodzi, pogwiritsa ntchito ma antibodies amitundu. Kenako makinawo amatha kuwerengera, kusanja, ndikulekanitsa maselo. Izi zimagwiritsidwa ntchito kuzindikira khansa monga khansa ya m'magazi ndi lymphoma, matenda osiyanasiyana opatsirana, ndi matenda odziteteza ku matenda . |
| Kujambula kwa immuno-electron microscopy (EM immunolabeling) | Izi zimathandiza kuti chitsanzo chiwonekere bwino kwambiri, mwatsatanetsatane. Apa, tinthu tagolide ta colloidal timalumikizidwa ku ma antibodies. Tikayang'ana pogwiritsa ntchito maikulosikopu ya elekitironi, tinthu tagolideti timaoneka bwino ngati madontho akuda. Chifukwa chake, antibody ikalumikizidwa ku antigen, imatha kuzindikirika mosavuta. Njirayi imagwiritsidwanso ntchito kuzindikira matenda opatsirana ndi mavairasi komanso kupeza malo omwe ma antigen a mavairasi ali m'katemera. |
Kodi ndiyenera kukonzekera bwanji mayesowa?
Kawirikawiri, simukuyenera kukonzekera chilichonse chapadera pa mayeso a chitetezo chamthupi.
Komabe, musanatenge chitsanzo (monga kutenga magazi kapena minofu, monga biopsy), dokotala wanu adzakupatsani malangizo omveka bwino okhudza zomwe mungachite. Mwachitsanzo, ngati mukuchitidwa biopsy, mungauzidwe kuti musadye kapena kumwa chilichonse musanachite opaleshoniyi. Ndikofunikira kutsatira malangizo amenewo molondola.
Kodi mayesowa amatenga nthawi yayitali bwanji? Kodi zotsatira zake zidzapezeka liti?
Nthawi yomwe imatenga kuti izi zichitike imasiyana malinga ndi njira yomwe yagwiritsidwa ntchito.
- Njira zina zimatha kupanga zotsatira m'maola ochepa .
- Njira zina zingatenge masiku 10 .
Pa tsiku limene mwatenga chitsanzo chanu, dokotala kapena wogwira ntchito adzakupatsani lingaliro la nthawi yomwe zingatenge kuti mupeze zotsatira, choncho musadandaule nazo.
Kodi zotsatira zake zikuti chiyani?
Katswiri wa matenda ku labotale adzayang'ana chitsanzo chanu ndikutumiza lipoti lonse la zomwe mwapeza kwa dokotala wanu. Kenako dokotala wanu adzagwirizanitsa zomwe zapezeka mu lipotilo ndi matenda anu ndikukufotokozerani mwachidule tanthauzo lake.
Kodi chimachitika ndi chiyani ngati zotsatira zake sizili zachilendo?
Ngati mayesowo atsimikizira kuti muli ndi vuto la matenda, musachite mantha. Dokotala wanu adzakufotokozerani zotsatira zake zonse ndikukambirana njira zomwe mungasankhire chithandizo.
Nthawi zina, gulu la madokotala ochokera m'magawo osiyanasiyana lingakumane kuti akambirane za njira yabwino kwambiri yothandizira. Cholinga cha aliyense ndikukupatsani chithandizo chabwino kwambiri. Chifukwa chake, lankhulani ndi dokotala wanu za mafunso kapena nkhawa zilizonse zomwe mungakhale nazo.
Uthenga Wopita Nawo Kunyumba
- Kuyezetsa thupi (immunostaining) ndi njira yapadera yoyezetsera thupi yomwe imagwiritsa ntchito ma antibodies kuti izindikire matenda monga khansa ndi matenda.
- Pali mitundu yosiyanasiyana ya mayeso awa, ndipo dokotala wanu adzasankha njira yoyenera kwambiri kutengera matenda anu.
- Dokotala wanu adzakuuzani malangizo oti mutsatire musanapereke chitsanzo kuti muyesedwe.
- Nthawi yomwe imatenga kuti munthu alandire zotsatira za mayeso imatha kusiyana, ndipo zotsatira zake zikapezeka, dokotala wanu adzakufotokozerani ndikukambirana njira zotsatirazi, ngati pakufunika kutero.
- Kambiranani momasuka ndi dokotala wanu mafunso aliwonse okhudza zotsatira za mayeso anu kapena matenda anu.
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