Ni dɔgɔtɔrɔ y’a fɔ aw ye ko aw ka sɛgɛsɛgɛli kɛ min tɔgɔ ye ko `Flow Cytometry`, aw bɛ se ka siran dɔɔni ni aw ye a tɔgɔ mɛn. N’a sɔrɔ i b’i yɛrɛ ɲininka ko: “Nin kɔrɔbɔli tɔgɔ kabakoma in ye mun ye?” Nka kun t’a la ka siran. A man gɛlɛn walima a tɛ siran i n’a fɔ i bɛ se k’a miiri cogo min na. N’an y’a fɔ cogo nɔgɔman na, o ye laboratuwari sɛgɛsɛgɛli kɛrɛnkɛrɛnnen ye min bɛ an farikolo kɔnɔfɛnw lajɛ ka ɲɛ kosɛbɛ. An ka kuma o kan cogo jɛlen na ani cogo nɔgɔman na.
N’an y’a fɔ cogo nɔgɔman na, `Flow Cytometry` ye mun ye?
Miiri k’a filɛ, biɲɛw kulu dɔ b’i bolo minnu kulɛriw tɛ kelen ye. Aw ka kan ka bilenmanw dɔrɔn jate ani ka u faranfasi ani ka buluw dɔrɔn de fara ɲɔgɔn kan. Nin sɛgɛsɛgɛli in min bɛ wele ko `Flow Cytometry`, o bɛ o ɲɔgɔn kɛ, nka biɲɛ nɔ na, a bɛ baara kɛ ni selilɛriw ye minnu bɛ bɔ an farikolo la.
Nin ye kɔrɔbɔli ye min bɛ baara kɛ ni fɛɛrɛ kuraw ye minnu ka bon kosɛbɛ. Aw bɛ min kɛ yan, o ye ko joli, kolotuguda walima farikolo yɔrɔ dɔ la, farikolokisɛ minnu bɔra aw la, olu bɛ bila ji la ka ci masin kɛrɛnkɛrɛnnen dɔ kɔnɔ . Nin masin in bɛ o seliw sɛgɛsɛgɛ kelen kelen, teliya la kosɛbɛ. O masin bɛ se ka selilu 10 000 ɲɔgɔn sɛgɛsɛgɛ miniti kelen kɔnɔ.
Dɔgɔtɔrɔw bɛ baara kɛ ni nin sɛgɛsɛgɛli in ye kosɛbɛ fɛnw na i n’a fɔ:
- Jate seliw: Seli suguya kɛrɛnkɛrɛnnen dɔ hakɛ tigitigi jate min bɛ misali dɔ kɔnɔ.
- Sort cellules: Aw bɛ seli suguya wɛrɛw fara ɲɔgɔn kan ani k’u dɔn.
- Aw bɛ farikolokisɛw taamacogo n’u ka baara lajɛ: Aw bɛ a lajɛ ni farikolokisɛw ka kɛnɛ ani ni fɛn dɔw b’a la ka yɛlɛma u ka baara la.
- Aw bɛ joli ni kolotugudaw kansɛriw sɛgɛsɛgɛ : O nafa ka bon kosɛbɛ banaw sɛgɛsɛgɛli ni u furakɛli bolodacogo la i n’a fɔ jolidɛsɛ ni jolidɛsɛ.
- Aw bɛ biyomarkɛriw ɲini: Aw bɛ fɛn dɔw (markɛriw) dɔn minnu bɛ taamasiyɛnw di kansɛri kɛcogo kan walima a jaabi furakɛli la.
- Immunophenotyping: Aw bɛ farikolokisɛ suguya wɛrɛw dɔn an ka farikolo tangacogo la.
O sɛgɛsɛgɛli bɛ kɛ tuma jumɛn?
Nin sɛgɛsɛgɛli in ka ɲi ni dɔgɔtɔrɔ b’a fɛ ka fɛn caman dɔn aw farikolo kɔnɔ farikolokisɛw kan. Kɛrɛnkɛrɛnnenya la, ni aw ye fɛn dɔw kɔlɔsi joli sɛgɛsɛgɛli kɛcogo kɔrɔ la (Full Blood Count - FBC/CBC), o sɛgɛsɛgɛli bɛ se ka yamaruya ka kɛ fɛɛrɛ nata ye. Misali la, ni FBC ka kunnafoni y’a jira ko dɔ farala joli-sira-funu hakɛ kan, o b’a jira ko joli-sira-funu-kɔgɔbaliw bɛ yen, nin sɛgɛsɛgɛli in bɛ se ka o lajɛ ka taa a fɛ.
Koba damadɔ bɛ yen minnu na nin kɔrɔbɔli in nafa ka bon:
- Joli kansɛriw : Ka bana dɔw sɛgɛsɛgɛ i n’a fɔ jolidɛsɛ ni jolidɛsɛ ani ka u suguya tigitigi dɔn.
- Jolilabana wɛrɛw: Walasa ka bana dɔw sɛgɛsɛgɛ minnu man ca, nka minnu ka jugu ni joli ye.
- Banakisɛfagalanw ka baara : Ni bana dɔw kɛra i n’a fɔ sidabanakisɛ walima farikolo tangacogo bangenen, aw bɛ farikolo tangalanw hakɛ ni u baara jateminɛ.
- Kansɛri furakɛli la : Nin sɛgɛsɛgɛli in fana nafa ka bon kosɛbɛ walasa ka kansɛri suguya lajɛ (a ka jugu cogo min na), ni a bɛ furakɛli kɛrɛnkɛrɛnnen dɔ jaabi, ani ni bana in `seginna` furakɛli kɔfɛ.
`Flow Cytometry` sɛgɛsɛgɛli bɛ baara kɛ cogo di tiɲɛ na?
Hali n’o tɔgɔ ka gɛlɛn, a kɛcogo ka nɔgɔn kosɛbɛ n’i y’a faamuya senfɛ-senfɛ. A bɛna kɛ lafiyaba ye i bolo k’a dɔn o bɛna min kɛ i ye.
| Taabolo minnu bɛ kɛ | Min bɛ kɛ (nɔgɔya la) . |
|---|---|
| 1. Ka misali sɔrɔ | Aw ka dɔgɔtɔrɔ bɛna aw joli, kolotugudaw walima farikolo yɔrɔ dɔ ta ka taa a bila laboratuwari la walasa ka a sɛgɛsɛgɛ. |
| 2. Seli labɛncogo | Laboratowari kɔnɔ, o selilɛri in bɛ wuli ji la. O kɔfɛ, selilu suguya kɛrɛnkɛrɛnnenw bɛ "tag" kɛ ni kalali kɛrɛnkɛrɛnnenw ye minnu bɛ yeelen bɔ. A bɛ i n’a fɔ ka stika kulɛri suguya wɛrɛw bila seli suguya wɛrɛw kan. |
| 3. Ka don masin kɔnɔ | Nin selilu ɲagaminen min nɔgɔlen don ni kalali ye, o bɛ don Flow Cytometer kɔnɔ . |
| 4. Laser-fiɲɛ ni sɛgɛsɛgɛli | Masina bɛ laser yeelen dɔ tɛmɛ seliw kɔnɔ, kelen kelen, ka tugu ɲɔgɔn na. Masina bɛ yeelen jɛnsɛncogo suman n’a bɛ seli kelen-kelen bɛɛ gosi. O jɛnsɛnni cogoya bɛ se ka kɛ ka seli suguya, a bonya ani a gɛlɛya dɔn. |
| 5. Kunnafoni-falen-falen | Kunnafoni minnu bɛ sɔrɔ, olu bɛ sɛgɛsɛgɛ ɔridinatɛri fɛ, ka kunnafoni dɔ dilan ka kɛ jatebɔsɛbɛnw ni kunnafonidilanw ye. |
| 6. Rapport ɲɛfɔli | O baara kɛrɛnkɛrɛnnen dɔ, walima banakɔtaa dɔnbaga , bɛna nin kunnafoni in sɛgɛsɛgɛ, k’u ka dantigɛliw don a kɔnɔ, ka kunnafoni dafalen ci aw ka dɔgɔtɔrɔ ma. |
Min nafa ka bon kosɛbɛ, o ye ko nin wale in bɛɛ bɛ kɛ laboratuwari kɔnɔ. I ka kan ka min kɛ dɔrɔn, o ye ka misali di. O la, aw man kan ka hami nin masin in na walima a kɛcogo la.
O jaabiw bɛ mun fɔ?
A bɛ se ka tile damadɔ walima dɔgɔkun kelen kɛ walasa ka a nɔw sɔrɔ. Banakisɛfagalan bɛna taamasiɲɛw walima antigènes (banakisɛfagalanw) cogoyaw lajɛ kɛrɛnkɛrɛnnenya la aw ka farikolokisɛw sanfɛ.
- Kɔnɔnatumu kɛnɛmanw: Kɔnɔnatumu kɛnɛman bɛ ni taamasiɲɛw cogoya ye min bɛ bɛn o farikolokisɛ suguya ni o kɔgɔli ma.
- Kɔnɔnatumuw: Kɔnɔnatumu dɔ, i n’a fɔ kansɛribana, taamasiɲɛw cogoya tɛ kelen ye, min tɛ deli ka kɛ.
`Banako dɔnbaga` bɛ kunnafoni labɛn ni o misaliw dɔnni ye.
Ni aw ka dɔgɔtɔrɔ ye nin kunnafoni in sɔrɔ, a tɛ a lajɛ dɔrɔn ka desizɔn ta. A b’a suma ni i ka bana taamasiɲɛw ye, i ka kɛnɛyako bɛɛ lajɛlen, ani farikolo sɛgɛsɛgɛli fɛn o fɛn kɛra kɔsa in na . O dɔrɔn de la, a b’a ɲɛfɔ i ye o nɔfɛkow kɔrɔ ye min ye.
Jateminɛ minnu tɛ kɛ cogo la, olu bɛ se ka bana dɔw jira i n’a fɔ:
- Jolidɛsɛ jugumanba min bɛ wele ko lymphoblastique
- Jolidɛsɛ miyɛloyi jugumanba
- Jolidɛsɛ basigilen min bɛ sɔrɔ lanposoti fɛ
- Miyɛloma caman
- Limfoma minnu tɛ Hodgkin ye
Ni o nɔw ma kɛ cogo la, mun bɛ kɛ o kɔfɛ?
Ni fɛn dɔw bɛ a sɔrɔcogo la, aw ka dɔgɔtɔrɔ bɛna baro kɛ o kan ni aw ye ka ɲɛ ani ka fɛɛrɛ nataw ni furakɛli suguyaw ɲɛfɔ.
Nin `Flow Cytometry` sɛgɛsɛgɛli in jaabiw nafa ka bon kosɛbɛ, bawo a bɛ kunnafoni nafamaw di fɛnw kan i n’a fɔ:
- Bana in ka jugu cogo di?
- Yala furakɛli kɛrɛnkɛrɛnnen dɔ bɛna ɲɛ wa?
- Bana in seginni farati ye mun ye furakɛli kɔfɛ?
Furakɛli bolodacogo min bɛ bɛn aw ma kosɛbɛ, o bɛ labɛn ni nin ko ninnu bɛɛ jateminɛ ye.
Tuma dɔw la, sɛgɛsɛgɛli caman ka kan ka kɛ walasa ka bana dɔ sɛgɛsɛgɛ ka ɲɛ. A bɛ se ka kɛ i sɛgɛn hakili ni farikolo ta fan fɛ. Nka aw hakili to a la ko kunnafoni minnu bɛ sɔrɔ sɛgɛsɛgɛli la i n’a fɔ `Flow Cytometry`, olu nafa ka bon kosɛbɛ aw ka dɔgɔtɔrɔ bolo. A tɛ dɛmɛ dɔrɔn bana sɛgɛsɛgɛli la, nka a bɛ dɛmɛ fana ka furakɛli bolodacogo `mɔgɔ yɛrɛ ta` labɛn min kɛrɛnkɛrɛnnen don i ma.
Take-Home cikan
- `Flow Cytometry` tɛ opereli walima fɛɛrɛ ye min bɛ siran mɔgɔ ɲɛ. O ye sɛgɛsɛgɛli kɛrɛnkɛrɛnnen ye min bɛ kɛ laboratuwari kɔnɔ ni sɛgɛsɛgɛli ye min bɔra aw la.
- Nin sɛgɛsɛgɛli in bɛ kunnafoni caman di aw ka farikolokisɛw kan, o min nafa ka bon kosɛbɛ joli kansɛriw ni farikolo tangacogo gɛlɛyaw sɛgɛsɛgɛli la.
- Aw ka kan ka min kɛ dɔrɔn, o ye ka sɛgɛsɛgɛli kɛ, i n’a fɔ joli. A tɔ bɛ kɛ laboratuwari kɔnɔ.
- Ni aw ye aw ka sɛgɛsɛgɛli rapɔɔri sɔrɔ, aw kana a ɲini k’a kalan ani k’a faamu aw yɛrɛ ma. Aw bɛ kuma aw ka dɔgɔtɔrɔ fɛ o ko la tuma bɛɛ. Ale de bɛna kɛ sababu ye ka jaabiw ni aw ka bana kɛcogo bɛn ɲɔgɔn ma, k’u ɲɛfɔ ka jɛya, ka aw ladɔnniya fɛɛrɛ nataw la.
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