Wan stori bɔt wi sɛl dɛn: Lɛ wi lan bɔt di Flɔ Saytometri tɛst insay wan simpul we!

Yu dɔn ɛva yɛri bɔt wan tɛst we dɛn kɔl Flow Cytometry? Sɔntɛm yu dɔktɔ dɔn tɛl yu bɔt dis tɛst. Ɔ sɔntɛm na nyu tin to yu. Bɔt na wan rili impɔtant tɛst we de ɛp wi fɔ no bɔku tin bɔt di sɛl dɛn na wi bɔdi. Tide, wi go tɔk bɔt am simpul wan we yu go ɔndastand.

Wetin na Flɔ Saytometri?

Fɔ tɔk am simpul wan, flɔ saytometri na lɛbɔraytri tɛst we dɛn kin yuz fɔ no di ditel dɛn bɔt sɛl ɔ smɔl smɔl tin dɛn na wi bɔdi. Tink bɔt am lɛk smɔl ditektiv we de luk insay wi sɛl dɛn.

Fɔ du dis, fɔs yu kin sɔlv wan sɛmpul pan yu sɛl dɛn (we kin bi blɔd, bon mɛro, ɔ ɔda tisu) insay wata. Dɔn, wan man we de wok na di lɛb kin ad spɛshal fluorescent antibodi dyes to di sɛl dɛn. dis day dεm de no εn atak spεshal protin dεm we de pan כ insay di sεl dεm. In ɔda wɔd dɛn, dɛn kin put smɔl lɛbl ɔ ‘tɛg’ pan dɛn prɔtin dɛn de. sכmtεm dεn kכl dis prכsεs Fluorescence-Activated Cell Sorting (FACS) .

Dɔn dɛn kin put dɛn sɛl dɛn ya we dɛn dɔn pripia insay wan mashin we dɛn kɔl flɔ saytomita. Di wɔndaful tin na dat, di kɔmpyuta we dɛn kɔnɛkt to dis mashin kin ebul fɔ analayz lɛk 10,000 sɛl dɛn insay jɔs wan minit! Yu nɔ tink se dat kin rili fast?

So, wetin dɛn kin yuz dis tɛst fɔ?

Dɔktɔ dɛn kin yuz dis flɔ saytometri tɛst fɔ bɔku tin dɛn. Di men wan:

• fכ kכnt di sεl dεm: Fכ no כl di sεl dεm we de insay wan sεmpl.
• sכt sεl dεm: Separet εn no difrεn kayn sεl dεm.
• Fɔ no aw di sɛl dɛn tan ɛn aw i de wok: Fɛn tin dɛn lɛk di shep we sɛl gɛt ɛn aw i de wok.
• Fɔ no ɛn trit blɔd ɛn bon mɛro kansa: Dis rili impɔtant fɔ no sik dɛn lɛk lukimiya ɛn limfoma.
• Fɔ fɛn bayɔmakɛr: Dɛn tin ya na tin dɛm wae kin no aw kansa go biev ɛn aw i go ansa to tritmɛnt.
• Immunophenotyping: fכ no εn klas di difrεn kayn imyun sεl dεm na wi bכdi.

Ustɛm dɛn kin du di flɔ saytometri tɛst?

Dɔktɔ dɛn kin yuz dis tɛst fɔ gɛt dip infɔmeshɔn bɔt di sɛl dɛn we de insay wi bɔdi. Speshal wan:

• Lan bɔt di sɛl dɛn we de na di imyun sistɛm: Si ɔmɔs sɛl dɛn we de fɛt sik na wi bɔdi ɛn us kayn dɛn.
• No di kansa sel dεm εn כndastand aw dεn tan: Fכ no aw kansa sεl dεm difrεn frכm כda sεl dεm εn wetin dεn bi.
• Risach kɔmpleks mɛdikal kɔndishɔn dɛn:
• Blɔd kansa: Fɔ ɛgzampul, sik dɛn lɛk lukimiya ɛn limfoma.
• Ɔda blɔd dizayd: Fɔ ɛgzampul, tin dɛn lɛk paroxysmal nocturnal hemoglobinuria (PNH) ɛn antithrombin dɛfisiɛns.
• di imyun sistεm fכ wok: Chεk fכ sik dεm lεk AIDS (HIV) εn di imyun sistεm dεm we dεn gεt wit inhεrit.

Wan ɔda tin na dat, sɔmtɛm afta dɛn dɔn du yu kɔmplit blɔd kɔnt (CBC) , if dɛn nid mɔ infɔmeshɔn bay di rizɔlt, dɛn kin du dis flɔ saytometri tɛst. spεshal wan, if di CBC sho inkrεs pan wan kayn sεl we dεn kכl limfosayt, if abnכmal tin de na כda sεl kכnt dεm, כ if i sho se blכd sεl dεm we nכ machכ de, dεn kin כda dis tεst.

Dis tɛst rili impɔtant nɔto fɔ no bɔt kansa nɔmɔ, bɔt fɔ trit kansa bak. Dat na:

• Gɛs aw di agresiv we di kansa gɛt tan lɛk.
• Fɔ no if di kansa go ansa wan patikyula tritmɛnt .
• Dis kin ɛp bak fɔ no if di kansa dɔn kam bak afta dɛn dɔn trit am.

Aw dis flɔ saytometri mashin de wok? I rili wɔndaful!

dis fכlכ saytכmetri mashin de analכz di fכs εn kεmikכl prכpati dεm fכ di sεl dεm kwik kwik wan. Tink bɔt am lɛk se yu de luk sɛl dɛn tru wan kamɛra we fast pasmak. Na dis na aw i de wok:

1. Fכ pripia di sεmpl: fכs, di lab tεknishian de tek sεmpl fכ yu blכd, tisu, כ bon mכro εn sכlv am insay wan likwid (dεn kכl am `suspension` ). afta dat, lεk aw wi bin dכn tכk, dεn de ad wan spεshal day we dεn kכl `fluorescently tagged antibodies` to di sεl dεm. afta dat dεn kin put di sεl sכlushכn we dεn pripia insay di fכlכ saytכmita.

2. Sεl alaynsmεnt εn lεzεr bim: Insay di mashin, dεn sεl dεm ya de arenj insay wan rכw, wan afta di כda. Imajin, lɛk we dɛn de layn skul pikin dɛn. Dɔn, dis row we gɛt sɛl dɛn kin pas bifo wan lɛsa bim, wan bay wan. We di lɛsa bim nak di sɛl dɛn, di layt kin skata, dat na layt we de skata.

3. Kכnt εn sכt sεl dεm: di fכlכ saytomita de kכnt sεl dεm εn kכl dεm bay di we aw di lεzεr bim de skata layt we i hit di sεl dεm. Ɛni kayn sɛl kin skata layt difrɛn we. Di mashin de du dis bay we i de no da difrɛns de.

4. 4. .Data to kɔmpyuta: Ɔl di infɔmeshɔn (data) we di mashin gɛda dis we, dɛn kin sɛn am to kɔmpyuta we kɔnɛkt to am.

5. Ripɔt jenɛreshɔn: Di kɔmpyuta de analayz dis data ɛn mek wan ripɔt, bɔku tɛm na dot ɔ bar grafik .

6. Patɔlɔjis in analisis: Fɔ dɔn, wan patɔlɔjis – we na mɛdikal spɛshal pɔsin we de no bɔt sik dɛn – de chɛk dɛn grafik ɛn data ya ɛn ad wan dɔkyumɛnt we gɛt wetin i fɛn ɛn wetin i dɔn tɔk to di ɛgzamin ripɔt.

Wetin a fɔ du bifo a du flɔ saytometri tɛst? Ɛnitin de we wi fɔ fred?

Bɔrku tɛm, pas yu dɔktɔ gi yu spɛshal instrɔkshɔn, nɔr spɛshal prɛparashɔn nɔr nid bifo dis flɔ saytometri tɛst.

Yu dɔktɔ go nid fɔ tek blɔd, tisu, ɔ bon mɛro fɔ du dis tɛst. Bɔku tɛm yu kin tek dis sampul na yu dɔktɔ in ɔfis we yu de go. Afta dɛn dɔn tek di sampul, dɛn kin sɛn am na lab fɔ mek dɛn tɛst am.

We di patɔlɔjis dɔn dɔn di flɔ saytometri tɛst, i go analayz di data ɛn sɛn ripɔt to yu dɔktɔ we aks fɔ di tɛst.

di mכst imכtant tin na dat, nכ risk dεm we dεn no naw fכ yu fכ du dis fכ fכlכ saytכmetri tεst. So nɔ fred dis we yu nɔ nid fɔ fred.

Aw di tin dɛn we kin apin kin tan lɛk? Wetin dɛn de tɛl wi?

I kin tek sɔm dez to wan wik fɔ gɛt di rizɔlt fɔ wan flɔ saytometri tɛst, i kin dipen pan di lab we yu dɔktɔ de yuz.

Di dɔktɔ we de mɛn di sik kin luk di mak dɛn we de na yu sɛl dɛn, we dɛn kɔl antijen .

• wan hεlty sεl gεt nכmal antijεnik patεn we fit fכ da kayn sεl de εn in stej fכ divεlכpmεnt.
• Dis patεn de difrεn na wan sεl we nכ nכmal . Dɛn chenj ya kin sho se yu gɛt lukimiya, lymphoma, ɔ ɔda mɛrɛsin.

We yu dɔktɔ gɛt di tɛst ripɔt, i go tek tɛm luk di patɔlɔjis in not, we na sɔm pan di data we di kɔmpyuta dɔn analayz frɔm di flɔ saytomita.

We yu de ɛksplen yu flɔ saytometri rizɔlt, yu dɔktɔ go tink bɔt yu sik dɛn, yu wɛlbɔdi istri, ɛn wan ɛgzam we yu jɔs dɔn du . I go ɛksplen to yu di tin dɛn we dɔn apin ɛn ɛksplen wetin dɛn min. I go yuz dis infɔmeshɔn bak fɔ disayd wetin fɔ du nɛks.

Us kayn sik dɛn we nɔrmal rizɔlt kin sho?

If di rizɔlt nɔr nɔrmal, i kin sho se yu gɛt tin dɛm lɛk:

• Akyu limfoblastik lukimiya
• Akyu mayloid lukimiya
• Kronik limfosaytik lukimiya
• Bɔku bɔku mayloma
• di nכn-Hכdgkin limfoma dεm (dεn kin inklud כl tu di B-sεl εn T-sεl tכp dεm) .

If di tin dɛn we kin apin nɔ kin fayn, wetin kin apin afta dat?

Yu dɔktɔ go tɔk bɔt yu flɔ saytometri rizɔlt ɛn di tritmɛnt opshɔn dɛn we yu kin gɛt wit yu. Dis tɛst ripɔt go ɛp yu dɔktɔ fɔ ɔndastand aw yu sik de ambɔg yu, if wan patikyula tritmɛnt fayn fɔ am, ɛn aw i go kam bak afta yu dɔn trit am . I go yuz ɔl dis infɔmeshɔn fɔ mek wan tritmɛnt plan we go fayn fɔ yu.

Sɔm sik dɛn kin tek sɔm tɛm fɔ no if dɛn gɛt am. Yu kin dɔn gɛt bɔku tɛst, bɔt yu stil nɔ gɛt di rayt ansa, ɛn yu kin de wɔnda se, "Wetin if a du ɔda tɛst?" Bɔt biliv mi, dis flɔ saytometri tɛst kin gi yu dɔktɔ bɔku valyu infɔmeshɔn bɔt di sɛl dɛn na yu bɔdi. Nɔto jɔs i go ɛp fɔ no di sik, bɔt i go ɛp yu bak fɔ mek yu yon tritmɛnt plan we go spɛshal fɔ yu.

So, wetin na di tin dɛn we wi nid fɔ mɛmba frɔm ɔl dɛn tin ya? (Mɛsej we dɛn kin tek go na os)

Okay, mek a sɔma sɔm pan di impɔtant pɔynt dɛn frɔm wetin wi dɔn tɔk bɔt:

• Flɔ saytometri na wan tɛst we rili advans we de stɔdi di sɛl dɛn dip wan.
• Dis kin ɛp fɔ no if pɔsin gɛt kansa na blɔd, prɔblɛm dɛn we gɛt fɔ du wit di imyun sistɛm, ɛn bɔku ɔda sik dɛn .
• Bɔku tɛm, di tɛst kin min fɔ tek blɔd, bon mɛro, ɔ tisu sɛmpul .
• Dis tɛst nɔ nid fɔ pripia bɔku bifo yu tek am, ɛn no risk nɔ de.
• De rizulyt de gi di dɔktɔ impɔtant infɔmeshɔn bɔt yu sik, di rayt tritmɛnt, ɛn aw di sik go go bifo .
• Dis na wan tin we rili valyu we de ɛp yu dɔktɔ fɔ mek wan tritmɛnt plan we go fayn fɔ yu .

So, if dɔktɔ tɛl yu fɔ du flɔ saytometri tɛst fɔ yu ɔ pɔsin we yu sabi, nɔ fred. Si am as we fɔ ɔndastand yu wɛl bɔdi fayn fayn wan ɛn ɛp yu fɔ gɛt di bɛst tritmɛnt. If yu gɛt ɛni kwɛstyɔn, aks yu dɔktɔ.

` Flɔ saytometri, sɛl tɛst, blɔd kansa, diagnosis, imuniti, mɛdikal tɛst, sɛl analisis