Ua kauoha paha kāu kauka i kahi hoʻāʻo kūikawā e hōʻoia i kahi hōʻailona. Ua lohe anei ʻoe i ka huaʻōlelo "immunostaining"? He mea paʻakikī a paʻakikī paha ia. Akā, he hoʻokolohua hoʻokolohua holomua loa ia e kōkua iā mākou e ʻike pololei i nā maʻi e like me ke kanesa a me nā maʻi like ʻole. I kēia lā, e kamaʻilio mākou e pili ana i ia mea ma ke ʻano maʻalahi loa.
I ka ʻōlelo maʻalahi, he aha ka Immunostaining?
ʻO ka Immunostaining kahi ʻano hana kūikawā i hoʻohana ʻia e ʻike i kahi molekala protein kikoʻī a ke kauka e makemake ai e nānā i waena o nā protein he nui i loko o kou koko, ka laʻana ʻiʻo, a i ʻole ka laʻana ʻiʻo iwi.
E noʻonoʻo e heluhelu ana ʻoe i kahi puke. Hoʻohana ʻoe i kahi peni highlighter e hōʻike i nā ʻōlelo koʻikoʻi loa i loko o ka puke i hiki iā ʻoe ke ʻike maʻalahi iā lākou. ʻO ia ka hana a kēia ʻano hana. Mai nā tausani o nā protein i loko o nā cell o ko mākou kino, kōkua kēia ʻano hana iā mākou e ʻike i nā protein kikoʻī (kapa mākou i kēia mau antigens ) e pili ana i kahi maʻi ma o ka "hoʻoluʻu ʻana iā lākou."
Hana ʻia kēia "haʻawe" me ka hoʻohana ʻana i nā mea kūikawā i kapa ʻia he antibodies . I mea e pololei ai, hoʻohana ʻia kahi antibody hiki ke hoʻopaʻa wale i kahi antigen kikoʻī. Hoʻopili ʻia kēia antibody i kahi pigment kūikawā, enzyme, mole fluorescent, a i ʻole nā ʻāpana gula. No laila, ke hoʻopaʻa kēlā antibody i ka antigen i nīnau ʻia, ke nānā mākou iā ia ma lalo o kahi microscope, lilo ia i kala nani a hiki iā mākou ke ʻike maopopo.
ʻO ka mea nui, hana ʻia kēia hoʻāʻo e nā pathologists i loko o kahi keʻena hoʻokolohua. ʻOiai ua lawe ʻia kahi hāpana (e like me ke koko a i ʻole ka ʻiʻo) mai ou aku ma ka haukapila, hana ʻia ka hoʻāʻo ma ka keʻena hoʻokolohua.
He aha nā ʻano nui o nā hoʻokolohua immunostaining?
Aia kekahi mau ʻano hana i hoʻohana ʻia e hana i kēia hoʻāʻo. E koho kāu kauka i ke ʻano hana kūpono loa ma muli o kou kūlana olakino. E nānā kākou i kekahi o nā ʻano hana nui.
| ʻAno hoʻāʻo | He aha ka hana a he aha ka mea i hoʻohana ʻia no kēia? |
|---|---|
| ʻO ke kemika Immunohistochemistry | ʻO kēia ke ʻano hana i hoʻohana pinepine ʻia. Maanei, ua kau ʻia kahi hāpana ʻiʻo i lawe ʻia mai ou aku ma kahi slide aniani, ua hoʻohui ʻia ka antibody kūpono, a ua hoʻohana ʻia kahi kala kūikawā e hoʻoluʻu iā ia. A laila ua nānā ʻia ma lalo o kahi microscope. He mea kōkua nui kēia i ka ʻike ʻana i nā ʻano maʻi kanesa like ʻole , ka hoʻokaʻawale ʻana i nā kūlana neurological, a me nā maʻi koko. |
| ʻO ke kahe ʻana o ka cytometry | E noʻonoʻo e like me ke scan ʻana i nā meaʻai ma kahi hale kūʻai nui. Ma kēia ʻano hana, hoʻouna ʻia kāu mau cell ma o kahi kukuna laser, hoʻokahi a hoʻokahi, me ka hoʻohana ʻana i nā antibodies kala. A laila hiki i ka mīkini ke helu, hoʻokaʻawale, a hoʻokaʻawale i nā cell. Hoʻohana ʻia kēia no ka ʻike ʻana i nā maʻi kanesa e like me ka leukemia a me ka lymphoma, nā maʻi lele like ʻole, a me nā maʻi autoimmune . |
| ʻO ka microscopy immuno-electron (EM immunolabeling) | ʻAe kēia i kahi hāpana e nānā ʻia ma ke kiʻekiʻe loa, ma ke ʻano kikoʻī loa. Maanei, ua hoʻopili ʻia nā ʻāpana gula colloidal i nā antibodies. Ke nānā ʻia ma lalo o kahi microscope electron, ʻike maopopo ʻia kēia mau ʻāpana gula ma ke ʻano he mau kiko ʻeleʻele. No laila, i ka wā e hoʻopili ai kahi antibody i kahi antigen, hiki ke ʻike maʻalahi ʻia. Hoʻohana ʻia kēia ʻano hana no ka ʻike ʻana i nā maʻi viral a no ka ʻike ʻana i nā wahi o nā antigens viral i nā lāʻau pale. |
Pehea e hoʻomākaukau ai au no kēia hoʻokolohua?
Ma keʻano laulā, ʻaʻole pono ʻoe e hoʻomākaukau i kekahi mea kūikawā no kahi hoʻāʻo immunostaining.
Eia nō naʻe, ma mua o ka lawe ʻana i kahi hāpana (ʻo ia hoʻi, ka lawe ʻana i ke koko a i ʻole kahi ʻāpana ʻiʻo, e like me ka biopsy), e hāʻawi kāu kauka iā ʻoe i nā ʻōlelo aʻoaʻo maopopo e pili ana i ka mea e hana ai. No ka laʻana, inā ʻoe e hana ana i kahi biopsy, e ʻōlelo ʻia paha ʻoe ʻaʻole e ʻai a inu paha i kekahi mea ma mua o ke kaʻina hana. He mea nui e hahai pololei i kēlā mau ʻōlelo aʻoaʻo.
ʻEhia ka lōʻihi o ka hoʻāʻo ʻana? Āhea e loaʻa ai nā hopena?
ʻOkoʻa ka manawa e hana ai kēia ma muli o ke ʻano i hoʻohana ʻia.
- Hiki i kekahi mau ʻōnaehana ke hoʻopuka i nā hopena i loko o kekahi mau hola .
- Hiki i kekahi mau ʻano hana ʻē aʻe ke lōʻihi a hiki i 10 mau lā .
I ka lā e lawe ʻia ai kāu hāpana, e hāʻawi ke kauka a i ʻole kekahi limahana iā ʻoe i kahi manaʻo no ka lōʻihi o ka manawa e loaʻa ai nā hopena, no laila mai hopohopo no kēlā.
He aha ka ʻōlelo a nā hualoaʻa?
E nānā ke kauka ma ka hale hana i kāu hāpana a hoʻouna i kahi hōʻike piha o nā ʻike i kāu kauka lapaʻau. A laila e hoʻopili kāu kauka i nā ʻike i loko o ka hōʻike i kou kūlana a wehewehe iā ʻoe ma nā huaʻōlelo maʻalahi i kona manaʻo.
He aha ka mea e hiki mai ana inā he ʻano ʻē nā hopena?
Inā hōʻoia ka hoʻāʻo i kahi maʻi, mai hopohopo. E wehewehe piha kāu kauka i nā hopena iā ʻoe a kūkākūkā i kāu mau koho lapaʻau.
I kekahi mau hihia, e hui pū paha kahi hui o nā kauka mai nā ʻoihana like ʻole e kūkākūkā i ka hoʻolālā lapaʻau maikaʻi loa. ʻO ka pahuhopu o kēlā me kēia kanaka ke hāʻawi iā ʻoe i ka lāʻau lapaʻau maikaʻi loa. No laila, e kamaʻilio me kāu kauka e pili ana i nā nīnau a hopohopo paha āu e loaʻa ai.
Leka Lawe-Home
- ʻO ka Immunostaining kahi hoʻokolohua hoʻokolohua kūikawā e hoʻohana ana i nā antibodies e ʻike pololei i nā maʻi e like me ke kanesa a me nā maʻi.
- Aia nā ʻano like ʻole o kēia hoʻāʻo, a na kāu kauka e koho i ke ʻano kūpono loa ma muli o kou kūlana olakino.
- E haʻi aku kāu kauka iā ʻoe i nā kuhikuhi e hahai ai ma mua o ka hāʻawi ʻana i kahi hāpana no ka hoʻāʻo ʻana.
- Hiki ke ʻokoʻa ka manawa e loaʻa ai nā hopena hoʻāʻo, a i ka wā e loaʻa ai nā hopena, e wehewehe kāu kauka iā ʻoe a kūkākūkā i nā hana aʻe, inā pono.
- E kūkākūkā ākea i kāu kauka i kekahi mau nīnau e pili ana i kāu mau hopena hoʻāʻo a i ʻole ke kūlana olakino.
💬 Comments (0)
No comments yet. Be the first to share your thoughts here.
Add Your Comment