Lɛ wi jɔs lan bɔt di immunophenotyping test we de ɛp fɔ no di kansa sɛl dɛn.

Yu nɔ go dɔn yɛri bɔt di wɔd `(Immunophenotyping)` bifo. Bɔt dis na tɛst we rili impɔtant, mɔ fɔ no sɔm sik dɛn, fɔ ɛgzampul, lukimiya, limfoma, ɛn ɔda kansa dɛn. So, tide wi go tok abaut dis test fo simpul we, so dat yu go andastan am gud gud wan.

Wetin na Immunophenotyping? Lɛ wi ɔndastand am simpul wan.

Okay, naw lɛ wi si wetin dis wɔd `(Immunophenotyping)` min. Pan ɔl we dis tan lɛk sayɛns wɔd smɔl, di stori rili simpul.

Di wɔd ‘Immuno’ de tɔk bɔt sɔntin we gɛt fɔ du wit wi imyun sistɛm . insay dis tεst, wan patכlayz (dכkta we spεshal fכ no εn trit sik dεm) de yuz כda kayn protin we dεn kכl ‘antibodies’ frכm wi כwn imyun sistεm fכ luk fכ spεsifi k protin dεm we dεn kכl ‘antigens’ insay wi sεl dεm. Tink bɔt dɛn ‘antibodi’ ya as ki, ɛn ‘antijen’ lɛk lɔk. lεk lכk we fit εvri ki, wan spεsifi k ‘antibodi’ go כnli atak wan spεsifi k 'antijen'. In ɔda wɔd, wan kayn pe de we de mach bitwin di tu.

di tεm we dεn kכl ‘fεnotayp’ de tכk bכt di kכntribyushכn dεm we yu kin si na do . so, we da `(antibodi)` de tay pan di rilevεnt `(antigen)`, di patכlayzm de no if da sεl de gεt da `(antigen)` de כ nכ de bays pan di sayn dεm we i de sho.

fכ sכmtεm, dis `(Immunophenotyping)` tεst de luk pan dεn `(antigens)` mak dεm we de na di sכfayz fכ wi sεl dεm εn fכ no if dεn sεl dεm na hεlty כ kεnsar sεl dεm. כlso, dεn `(antijen)` dεm kin no us kayn kεnsar sεl i bi.

Ustɛm dɛn kin du dis (Immunophenotyping) tɛst?

Bɔku tin dɛn de we yu dɔktɔ kin tɛl yu fɔ du dis tɛst.

Imajin se yu padi, lɛ wi se Nimal, dɔn de fil bad bad wan ɛn taya fɔ sɔm dez. We di dɔktɔ du blɔd tɛst (a ``Complete Blood Count'', ɔ ``CBC''), i si se in wayt blɔd sɛl kɔnt nɔ bin bɔku. Dɔn di dɔktɔ go tink se dis kin bi blɔd kansa. Na da tɛm de i go rɛkɔmɛnd fɔ du dis ``Immunophenotyping'' tɛst fɔ no mɔ bɔt wetin ɛksaktɔli de apin.

Dat min se:

• If di rizɔlt fɔ kɔmplit blɔd kɔnt (`(CBC)`) sho se yu wayt blɔd sɛl kɔnt nɔrmal.
• If pɔsin we dɛn tink se gɛt sɔm kayn blɔd kansa, fɔ ɛgzampul, lukimiya ɔ limfoma, dis kin ɛp fɔ kam nia smɔl nia di sik we dɛn no.

pan tap dat, risecharch dεm de yuz dis mεtכd bak fכ ``Immunophenotyping'' fכ stכdi aw kεnsar sεl dεm de rεspכnd to spεsifi k ``antigen'' mak dεm, spεshal wan ``immunotherapy''.

Aw dis tɛst de wok?

כl dis `(Immunophenotyping)` tεst de apin na lab, afta dεn tek tisu כ fluid sεmpl frכm yu. Fɔ tɔk am strikt wan, ɔl wetin yu fɔ du na fɔ gi di sampul, ɛn di masta sabi pipul dɛn na di lab de du di ɔda tin dɛn.

כl di dכkta dεm de yuz wan lab tεknik we dεn kכl ``Flow Cytometry'' fכ du dis tεst. pan tap dat, sכmtεm dεn kin yuz wan tεknik we dεn kכl ``Immunohistochemistry - IHC'' bak. Lɛ wi tek wan luk pan dɛn tu tin ya.

Tu men we dɛn de...

(Flow Cytometry) we dɛn kin yuz (we dɛn kin yuz mɔ) .

If yu yuz dis we, yu kin chɛk big big blɔd ɔ blɔd sɛl dɛn we dɛn tek frɔm di tisu dɛn. Na dis na aw i de wok:

• Fɔ gɛda di sɛmpul: Dis kin min fɔ tek wan sɛmpul pan bon mɛro frɔm bon mɛro bayɔpsi, ɔ smɔl blɔd. sכmtεm, dεn kin tek di sεmpl frכm spεnal fכluid כ fכ fכm we de na di bכdi.
• fכ kip di sεmpl: di lab wokman dεm de pripia di sεmpl fכ di ``Immunophenotyping'' tεst. Dɛn kin ad sɔm tin dɛn to am fɔ mek di sɛl dɛn nɔ day.
• ad (Antibody): nεks, dεn de ad di ``antibodies`` we dεn dכn disayn fכ biεn di ``antigen`` we dεn want fכ fכn. dis ``antibodi`` dεm de atak wan ``fכrosεnt kכmpawnd`` כ ``imyunofluoresεns mak``. biכs fכ dis mak, we di ``antibodi`` de tay pan di ``antijen``, di sεl dεm de kכmכt layt.
• fכ du (Flow Cytometry): Naw dεn put di sεmpl we dεn pripia wit dεn `(antibodies)` dεm insay wan mashin we dεn kכl `(Flow Cytometer)`. dis mashin gεt spεshal sεns dεm (`detectors`), we kin detekt di layt we de kכmכt frכm dat `(immunofluorescence marker)`. so, dis `(Flow Cytometer)` de mכsu aw mכl sεl dεm de we dεn bכnd `(antigen-antibody)` (i.e. emit layt).

(Immunohistochemistry - IHC) we de du tin

imyunohistochemistry na wan we we kin no if spεsifi k antijen dεm de insay wan tisu sεmpl. di stεp dεm we de insay di lab rili sכm lεk di wan dεm we de insay fכlכ saytכmetri. di men difrεns na dat insay IHC, dεn de ad wan εnzym to di antibodi dεm insted fכ wan fכrosεnt kכmpawnd. dis εnzym de stεn di sεl dεm wit wan spεsifi k kכla if di antijen de. If di patɔlɔjis si wan stɛyn ɔnda maykroskɔp, dat min se di sɛl dɛn gɛt di antijen.

Wetin na di limiteshɔn dɛm fɔ dis (Immunophenotyping) tɛst?

di big drawback na dat nכto evri lab we de du dis tεst gεt di sem ``standard prosidכs .'' So, sכmtεm sכmtεm i kin bi sכm vεryushכn na di risכlt.

Bɔt wan tin we yu fɔ mɛmba. Dɔktɔ dɛn nɔ kin no di sik bay dɛn `(Immunophenotyping)` rizɔlt ya nɔmɔ. Dɛn kin kɔmpia am wit bɔku ɔda tin dɛn, lɛk di sayn dɛn we yu kin gɛt ɛn di tin dɛn we kin kɔmɔt frɔm ɔda tɛst dɛn, fɔ mek dɛn ebul fɔ no sɔntin. Dis min se dis tɛst nɔmɔ nɔ de tɛl yu se yu gɛt kansa. Na jɔs wan pat pan big pikchɔ, jɔs wan sɔs we go ɛp yu fɔ lan mɔ bɔt yu wɛlbɔdi.

Aw di tin dɛn we kin apin kin tan lɛk? Wetin dɛn de tɛl wi?

Yu dɔktɔ go ɛksplen to yu wetin yu rizɔlt min bay di patɔlɔjis in analisis. di rizulεt dεm fכ wan ``Immunophenotyping'' tεst nכto jכs ``gud'' כ ``bad''. i tek wan tren pathologist fכ כndastand di ``data'' we di ``Flow Cytometer'' de jεnarεt bכt di sεmpl εn fכ intaprit wetin i se kכrekt wan.

Frɔm dis data, i de analayz tin dɛn lɛk:

• Wetin na di `tayp wayt blɔd sɛl`?
• Us stej fɔ divɛlɔp dɛn de insay?
• כl di sεl dεm gεt di antijen we yu de luk fכ?

Di patɔlɔjis kin kɔmpia di data frɔm yu sampul wit di data frɔm pipul dɛn we gɛt wɛlbɔdi ɛn we nɔ gɛt kansa, ɛn bak di data frɔm pipul dɛn we gɛt wan patikyula kayn lukimiya ɔ limfoma. We i yuz dɛn patɛns ya ɛn di rizɔlt fɔ ɔda tɛst dɛn, i de no aw yu go gɛt di kansa we yu de tɔk bɔt.

Ustɛm a fɔ kɔl mi dɔktɔ?

If yu gɛt ɛni kwɛstyɔn ɔ wɔri bɔt yu rizɔlt ɔ wetin dɛn min, mek shɔ se yu kɔl yu dɔktɔ . Immunophenotyping na komplex lab test we de luk di kכntribyushכn dεm fכ di sεl dεm. Bɔt yu dɔktɔ kin ɛksplen aw i kin afɛkt yu wɛlbɔdi insay simpul wɔd dɛn.

Sɔntɛm yu nɔ bin fil ɛni pen, ɛn yu bin sɔprayz fɔ no se yu wayt blɔd sɛl dɛn bin ay pan wan rutin blɔd tɛst (wan ``CBC''). Ɔ sɔntɛm yu dɔn de fil bad fɔ sɔm tɛm. Bɔt, dɛn ``CBC'' rizɔlt dɛn de na jɔs di fɔs step fɔ no wetin de apin, so yu go gɛt fɔ peshɛnt.

Yu sɛl dɛn gɛt bɔku impɔtant tin dɛn bɔt yu wɛlbɔdi. Immunophenotyping na test we de luk yu sel dεm εn tεl yu bכt yu hεlth. Sɔntɛnde lɛk dis, fɔ wet fɔ di tin dɛn we go apin kin rili mek yu fred ɛn mek yu wɔri. If yu nid sɔpɔt pan aw yu de fil, tɔk to yu dɔktɔ bɔt am. Dɔn bak, if yu gɛt ɛni kwɛstyɔn bɔt di tɛst rizɔlt, nɔ shek fɔ aks.

Di impɔtant tin dɛn we wi nid fɔ lan frɔm dis

Okay, so lɛ wi sɔmariz di impɔtant tin dɛm fɔ mɛmba bɔt dis `(Immunophenotyping)` tɛst we wi tɔk bɔt tide.

• Dis na spɛshal lab tɛst. I kin ɛp mɔ fɔ no bɔt kansa lɛk lukimiya ɛn limfoma.
• dis kin bi bay we dεn de no spεshal protin dεm we dεn kכl ``antijen'' na di sεf fכ wi sεl dεm.
• di tu we dεm we dεn kin yuz mכst na ``Flow cytometry'' εn ``Immunohistochemistry - IHC''.
• Di rizɔlt dɛn kin kɔmplikt smɔl, so wan dɔktɔ we de mɛn sik dɛn kin analayz dɛn.
• Yu fɔ mɛmba dis: Nɔbɔdi nɔ go tɛl yu se yu gɛt kansa jɔs bay we yu luk di rizɔlt fɔ dis tɛst. Na wan pat nɔmɔ pan di diagnostik prɔses.
• If yu gɛt ɛni kwɛstyɔn bɔt di rizɔlt, nɔ fred fɔ aks yu dɔktɔ. Dɛn de de fɔ ɛp yu.

` Imyunofenotayp, kansa, lukimiya, limfoma, blɔd tɛst, sɛl, antijen