Nyaya pamusoro pemasero edu: Ngatidzidzei nezvebvunzo yeFlow Cytometry nenzira iri nyore!

Wakambonzwa nezvebvunzo inonzi Flow Cytometry here? Pamwe chiremba wako akakuudza nezvebvunzo iyi. Kana kuti pamwe ichangobva kuitika kwauri. Zvisinei, bvunzo inokosha zvikuru inotibatsira kuwana ruzivo rwakawanda nezvemasero ari mumuviri wedu. Nhasi, tichataura nezvazvo nenzira iri nyore yaunogona kunzwisisa.

Chii chinonzi Flow Cytometry?

Zvichitaurwa zviri nyore, flow cytometry ibvunzo yemurabhoritari inoshandiswa kutsvaga ruzivo nezvemasero kana zvidimbu zvidiki mumiviri yedu. Funga nezvazvo semuongorori mudiki ari kutarisa mukati memasero edu.

Kuti uite izvi, tanga waisa muenzaniso wemasero ako (anogona kunge ari ropa, mupfupa, kana imwe nyama) inonyungudutswa mumvura. Zvadaro, nyanzvi yekurabhoritari inowedzera madhayi ekudzivirira masero e fluorescent kumasero. Madhayi aya anoziva uye anonamira kumapuroteni chaiwo ari mukati kana mukati memasero. Nemamwe mashoko, anonamira chidimbu chidiki, kana kuti 'tag,' kumapuroteni iwayo. Maitiro aya dzimwe nguva anonzi Fluorescence-Activated Cell Sorting (FACS) .

Masero aya akagadzirwa anobva aiswa mumuchina unonzi flow cytometer. Chinhu chinoshamisa ndechekuti, komputa yakabatana nemuchina uyu inogona kuongorora masero angangoita zviuru gumi muminiti imwe chete! Hazvisi zvekukurumidzira here izvozvo?

Saka, bvunzo iyi inoshandiswa pai?

Vanachiremba vanoshandisa bvunzo iyi ye flow cytometry pazvinhu zvakawanda. Zvikuru:

• Kuverenga masero: Tsvaga kuti masero mangani ari mumuenzaniso.
• Kuronga masero: Kuparadzanisa uye kuona mhando dzakasiyana dzemasero.
• Kuziva hunhu hwesero uye mashandiro aro: Tsvaga zvinhu zvakaita sechimiro chesero uye mashandiro aro.
• Kuongororwa uye kurapwa kwegomarara remuropa neremumapfupa: Izvi zvakakosha zvikuru kuti uzive zvirwere zvakaita seleukemia nelymphoma.
• Kutsvaga zviratidzo zvekenza: Izvi zviyero zvinogona kufanotaura kuti kenza ichaita sei uye kuti ichapindura sei kana yarapwa.
• Kugadzira masero ekudzivirira muviri: Kuronga uye kuona kuti ndeapi marudzi akasiyana emasero ekudzivirira muviri ari mumuviri wedu.

Kuongororwa kwe flow cytometry kunoitwa rini?

Vanachiremba vanoshandisa bvunzo iyi kuti vawane ruzivo rwakadzama nezvemasero ari mumiviri yedu. Zvikuru sei:

• Dzidza nezvemasero emuviri wemuviri: Ona kuti tine masero mangani anorwisa zvirwere mumiviri yedu uye kuti ndeapi marudzi awo.
• Ziva masero egomarara uye nzwisisa hunhu hwawo: Tsvaga kuti masero egomarara akasiyana sei nemamwe masero uye hunhu hwawo.
• Ongorora mamiriro ezvinhu akaomarara ehutano:
• Gomarara reropa: Semuenzaniso, zvirwere zvakaita seleukemia nelymphoma.
• Zvimwe zvirwere zveropa: Semuenzaniso, zvirwere zvakaita se paroxysmal nocturnal hemoglobinuria (PNH) uye antithrombin deficiency.
• Kushanda kwemasoja emuviri: Tarisa zvirwere zvakaita seAIDS (HIV) uye kushomeka kwemasoja emuviri anogarwa nhaka.

Chimwe chinhu ndechekuti, dzimwe nguva mushure mekunge ropa rako razara (CBC) rapera, kana paine rumwe ruzivo rwakawanda runodiwa zvichibva pane zvabuda, bvunzo iyi ye flow cytometry inogona kuitwa. Kunyanya, kana CBC ichiratidza kuwedzera kwerudzi rwesero runonzi lymphocytes, kana paine kusarongeka kwemamwe masero, kana kana ichiratidza kuvapo kwemasero eropa asina kuibva, bvunzo iyi inogona kurairwa.

Kuongororwa uku kwakakosha zvikuru kwete chete pakuongorora kenza, asiwo pakurapa kenza. Ndizvo:

• Fungidzira kuti kenza yacho inorwisa sei.
• Sarudza kana kenza ichapindura kurapwa kwakati .
• Izvi zvinobatsirawo kuona kana kenza yakadzoka mushure mekurapwa.

Muchina uyu we flow cytometry unoshanda sei? Zvinoshamisa!

Muchina uyu we flow cytometry unoongorora hunhu hwemuviri nemakemikari emasero nekukurumidza. Funga nezvazvo sekutarisa masero uchishandisa kamera inokurumidza zvikuru. Heino mashandiro ayo:

1. Kugadzirira sampuro: Kutanga, nyanzvi yekurabhoritari inotora sampuro yeropa rako, nyama, kana mupfupa woinyungudutsa mumvura (inonzi `suspension` ). Zvadaro, sezvambotaurwa, dhayi yakakosha inonzi `fluorescently tagged antibodies` inowedzerwa kumasero. Mhinduro yemasero yakagadzirwa inobva yaiswa mu flow cytometer.

2. Kurongeka kwemasero uye danda relaser: Mukati memuchina, masero aya akarongwa mumutsara mumwe chete, rimwe mushure merimwe. Fungidzira, sevana vechikoro vari mumutsara. Zvadaro, mutsara uyu wemasero unopfuura pamberi pedanda relaser, rimwe nerimwe. Kana danda relaser rarova masero, chiedza chinopararira, kureva chiedza chakapararira.

3. Kuverenga nekusarudza masero: flow cytometer inoverenga masero uye inoaisa muzvikamu zvichienderana nekuti laser beam inoparadzira sei chiedza painorova masero. Rudzi rumwe nerumwe rwesero runoparadzira chiedza zvakasiyana. Muchina unoita izvi nekuona mutsauko iwoyo.

4.Data kukombiyuta: Ruzivo rwese (data) runounganidzwa nemuchina nenzira iyi runotumirwa kukombiyuta yakabatana nayo.

5. Kugadzira mishumo: Kombiyuta inoongorora data iri uye inogadzira mushumo, kazhinji muchimiro chedoti kana girafu yebhawa .

6. Kuongororwa kwenyanzvi yezvirwere: Pakupedzisira, nyanzvi yezvirwere - nyanzvi yezvehutano inoongorora zvirwere - inoongorora magirafu aya nedata uye inowedzera gwaro rine zvaakawana uye mhedziso kumushumo wekuongorora.

Chii chandinofanira kuita ndisati ndaongororwa flow cytometry? Pane chekutya here?

Kazhinji, kunze kwekunge chiremba wako akupa mirairo yakakosha, hapana gadziriro yakakosha inodiwa usati watanga bvunzo iyi ye flow cytometry.

Chiremba wako achada sampuli yeropa, nyama, kana munyu wemapfupa kuti aite bvunzo iyi. Muenzaniso uyu unogona kutorwa kuhofisi yachiremba wako panguva yekushanya kwako. Mushure mekunge sampuli yatorwa, inotumirwa kurabhoritari kuti inoongororwa.

Kana chiremba wezvirwere apedza bvunzo ye flow cytometry, achaongorora data racho otumira mushumo kuna chiremba wako akakumbira bvunzo yacho.

Chinhu chinonyanya kukosha ndechekuti hapana njodzi dzinozivikanwa pari zvino dzaungasangana nadzo kubva pakuita bvunzo iyi ye flow cytometry. Saka usatya izvi zvisina basa.

Zvakaita sei? Zvinotiudza kuti chii?

Zvinowanzotora mazuva mashoma kusvika pavhiki kuti uwane mhedzisiro yeongororo ye flow cytometry, zvichienderana nerabhoritari inoshandiswa nachiremba wako.

Chiremba wezvirwere anotarisa zviratidzo zviri pamusoro pemasero ako, zvinonzi maantigen .

• Sero rine hutano rine maitiro akajairika ekudzivirira chirwere akakodzera rudzi rwesero iroro uye danho raro rekukura.
• Maitiro aya akasiyana musero risina kujairika . Kuchinja uku kunogona kuratidza kuvapo kweleukemia, lymphoma, kana zvimwe zvirwere.

Kana chiremba wako agamuchira mushumo wekuongororwa, achanyatsoongorora gwaro renyanzvi yezvirwere, iro riri pfupiso yedata rakaongororwa nekombiyuta kubva ku flow cytometer.

Pakududzira zvabuda muongororo ye flow cytometry yako, chiremba wako achaongorora zviratidzo zvako, nhoroondo yehutano hwako, uye bvunzo yemuviri yakaitwa nguva pfupi yadarika . Iye achakutsanangurira zvabuda uye achatsanangura zvazvinoreva. Iye achashandisawo ruzivo urwu kusarudza zvekuita zvinotevera.

Ndezvipi zvirwere zvinogona kuratidzwa nemhedzisiro isina kujairika?

Kana mhedzisiro yacho isina kujairika, inogona kuratidza zvirwere zvakaita se:

• Leukemia yeropa inouraya zvikuru
• Chirwere chemukondombera chinouraya myeloid leukemia
• Leukemia isingaperi yelymphocytic
• Multiple myeloma
• Non-Hodgkin lymphomas (idzi dzinogona kusanganisira mhando dzeB-cell neT-cell)

Kana mhedzisiro yacho isina kujairika, chii chinozoitika?

Chiremba wako achakurukura newe zvabuda muongororo yako ye flow cytometry pamwe nesarudzo dzekurapa dzinogona kuitwa. Mushumo uyu webvunzo uchabatsira chiremba wako kunzwisisa kuti chirwere chako chakanyanya sei, kana kurapwa kwakakodzera iwe, uye kuti kenza yako inogona kudzoka sei mushure mekurapwa . Iye achashandisa ruzivo urwu rwese kugadzira hurongwa hwekurapa hwakakunakira iwe.

Zvimwe zvirwere zvinotora nguva kuti zviongororwe. Ungave wakaongororwa kakawanda, asi hausati wawana mhinduro chaiyo, uye ungave uchishamisika kuti, "Ko kana ndikaita imwe bvunzo?" Asi nditende, bvunzo iyi ye flow cytometry inogona kupa chiremba wako ruzivo rwakawanda rwakakosha nezvemasero ari mumuviri wako. Hazvizongobatsiri chete kuongorora chirwere chacho, asi zvichakubatsirawo kugadzira hurongwa hwekurapa hwakagadzirirwa iwe pachako .

Saka, ndezvipi zvinhu zvatinofanira kurangarira kubva pane izvi zvese? (Mharidzo Yekuenda Kumba)

Zvakanaka, regai ndipfupise zvimwe zvezvinhu zvakakosha kubva pane zvatataura nezvazvo:

• Flow cytometry bvunzo yepamusoro-soro inodzidza masero zvakadzama.
• Izvi zvinobatsira kuona kenza yeropa, matambudziko ekudzivirira muviri, nezvimwe zvirwere zvakawanda .
• Kuongororwa kunowanzo sanganisira kutora ropa, mupfupa, kana tissue .
• Bvunzo iyi haidi kugadzirira zvakanyanya usati waitora, uye hapana njodzi.
• Mhedzisiro yacho inopa chiremba ruzivo rwakakosha nezvechirwere chako, kurapwa kwakakodzera, uye kuti chirwere chichaenderera mberi sei .
• Ichi chishandiso chinokosha zvikuru chinobatsira chiremba wako kugadzira hurongwa hwekurapa hwakakunakira .

Saka, kana chiremba akakurudzira kuti iwe kana mumwe munhu waunoziva muedze flow cytometry test, usatya. Ione senzira yekunzwisisa hutano hwako zviri nani uye kukubatsira kuti uwane kurapwa kwakanakisisa. Kana uine mibvunzo, bvunza chiremba wako.

` Kuyerera kwemasero, kuongororwa kwekenza yeropa, kuongororwa, dziviriro yemuviri, bvunzo dzekurapa, kuongororwa kwemasero