Chii chinonzi Immunostaining? Ngatitaurei nezvebvunzo iyi yekuona chirwere nenzira iri nyore!

Wakamboudzwa here kuti chiremba akupe bvunzo inonzi "Immunostaining"? Kana kuti wakambonzwa izwi iri pane imwe nzvimbo? Pamwe rinonzwika serakaoma kwauri. Asi usazvidya moyo, nhasi tichataura nezve bvunzo iyi inonzi Immunostaining, zvainoita, kuti zvinoitwa sei, zvese nenzira iri nyore yaunogona kunzwisisa. Sekutaura neshamwari.

Chii chaizvo chinonzi immunostaining?

Zvichitaurwa zviri nyore, immunostaining bvunzo yakakosha inoshandiswa nenyanzvi dzedu dzezvirwere. Inoshandiswa kuona puroteni chaiyo pakati pemapuroteni akawanda mumuenzaniso unotorwa mumuviri wako, senge ropa, muro wemapfupa, kana nyama. Funga nezvazvo sekutsvaga munhu chaiye muboka revanhu. Nzira iyi inobatsira zvikuru pakuongorora zvirwere zvakasiyana-siyana, kunyanya hutachiona nekenza.

Bvunzo iyi inoshanda sei?

Zvakanaka, ngationei kuti immunostaining iyi inoshanda sei. Inoshandisa chimwe chinhu chinonzi ma antibodies . Ma antibodies aya akafanana nemasoja ari mudziviriro yemuviri wedu. Anoziva uye anonamatira kuzvinhu zvinokonzera chirwere, zvinonzi ma antigen . Saka, mukuyedza uku, vanachiremba vanoshandisa antibody chaiyo yeprotein iyoyo (inonzi antigen) yavari kutsvaga.

Zvadaro kunouya chikamu che "kusvibisa". Zvakafanana nekujekesa mutsetse wakakosha mubhuku. Asi hazvisi nyore sekupendesa T-shirt. Nzira idzi dze "kusvibisa" dzinoshandisa madhayi akakosha, ma enzymes anochinja mavara, mamorekuru e fluorescent , uye kunyange zvidimbu zvegoridhe. Izvi zvinonyora mapuroteni nemasero avari kutsvaga, kuitira kuti vaongorori vagone kuzviona zvakajeka pasi pemaikorosikopu.

Chiremba asati aita bvunzo iyi yekudzivirira muviri, anotora sampuli yeropa rako, mupfupa, kana nyama. Ichazotumirwa kuna chiremba wezvirwere kuti aongororwe. Yeuka, kungave sampuli yacho yakatorwa kuchipatara kana muhofisi yachiremba, bvunzo iyi yekudzivirira muviri inogara ichiitwa murabhoritari, kana yatinoti rabhoritari.

Ndeapi marudzi ekudzivirira muviri?

Kune nzira dzakasiyana siyana dzekudzivirira muviri dzinoshandiswa navanachiremba. Nzira imwe neimwe ine nzira chaiyo yekuongorora, kana kuti protocol, ine chekuita nayo. Heano mamwe enzira huru dzinoshandiswa:

• Kudzivirira hutachiona hwemuviri
• Kuyerera kwemasero eropa
• Kuongororwa kwe immuno-electron microscopy (EM immunolabeling)

Tinofanira kutarisa chimwe nechimwe cheizvi zvakadzama here?

Immunohistochemistry - nzira inonyanya kushandiswa

Iyi ndiyo nzira inonyanya kushandiswa yekudzivirira muviri . Mu immunohistochemistry, chidimbu chidiki chemuenzaniso chinoiswa pagirazi rinotsvedza, uye antibody inosunga kune chinangwa inowedzerwa pairi. Yobva yaiswa madhayi chaiwo kana ma enzyme. Aya anonzi ma immunostain. Izvi zvinobvumira chiremba wezvirwere kuona mamwe mapuroteni nemasero zvakajeka pasi pemaikorosikopu.

Ona mabhenefiti e immunohistochemistry:

• Inobatsira kuziva mhando dzakasiyana dzegomarara.
• Inobatsira kupatsanura zvirwere zvakawanda zvetsinga nezvirwere zveropa.
• Izvi zvinobvumirawo vanachiremba kudzidza migumisiro, kana kuti matauriro, emajini mamwe.

Kuyerera kwemasero - semuchina unoverenga nekuparadzanisa masero

Munzira inonzi flow cytometry, nyanzvi inoshandisa ma antibodies ane mavara mavara kuratidza mapuroteni avari kutsvaga. Zvadaro, danda re laser rakakosha rinoshandiswa kuverenga, kurongedza, uye kuparadzanisa masero ako rimwe nerimwe. Zvakafanana nekutarisa zvekudya musupamaketi.

Vanachiremba vanoshandisa flow cytometry kuongorora zvirwere zvakaita se:

• Zvirwere zvinotapukira
• Zvirwere zve autoimmune
• Leukemia
• Lymphoma

Kuongororwa kwe immuno-electron microscopy (EM immunolabeling)

Microscopy yeImmuno-electron inobvumira chiremba wezvirwere kuona sampu yako nekukurisa kukuru uye zvakadzama. Muizvi, vanachiremba vezvirwere vanobatanidza zvidimbu zvidiki zvegoridhe zvinonzi colloidal gold particles kuma antibodies. Goridhe iyi yecolloidal iri nyore kuona uchishandisa electron microscope. Saka, kana antibody iyoyo ikaenda yobata antigen, zvidimbu zvegoridhe zvinoita kuti ionekwe zvakajeka semadonhwe matema.

Kuwedzera pakuona hutachiona hwakasiyana-siyana nezvirwere, immuno-electron microscopy inogonaKutowana maantigen ehutachiona mumajekiseni.

Ndinofanira kugadzirira sei bvunzo iyi?

Kazhinji, haufanirwe kugadzirira bvunzo yekudzivirira muviri. Zvisinei, chiremba wako anokupa mirairo usati watora sampuli yeropa rako, nyama, kana mumongo wemapfupa. Izvi zvichakubatsira kuziva chaizvo zvekutarisira. Zvakakosha kunyatsoteerera zvinotaurwa nachiremba wako.

Bvunzo yacho inotora nguva yakareba sei? Zvinotora nguva yakareba sei kuti mhinduro dzidzoke?

Izvi zvinoenderana nenzira inoshandiswa nachiremba wako yekudzivirira chirwere. Dzimwe nzira dzinongotora maawa mashoma chete , nepo dzimwe dzichitora kusvika mazuva gumi . Chiremba wako achakwanisa kukuudza kuti zvichatora nguva yakareba sei zvichienderana nemamiriro ako ezvinhu.

Nguva inotora kuti mhedzisiro idzoke yakafananawo. Dzimwe nzira dzekudzivirira muviri dzinogona kukupa mhedzisiro mukati mezuva rimwe chete. Dzimwe dzinogona kutora vhiki kana kupfuura. Paunoenda kunopa sampuro, chiremba wako achakuzivisa nguva yaunogona kutarisira mhedzisiro.

Saka, panguva yakaita seiyi, zvakanaka kusatya asi kuva nemoyo murefu. Taura nachiremba wotsanangura zvese.

Mhedzisiro yacho inoti chii? Chii chinoitika kana zvisina kujairika?

Chiremba wezvirwere achaongorora zvabuda mubvunzo yenyu ozvitumira kuna chiremba wenyu. Zvamunowana zvinogona kusiyana zvichienderana nerudzi rwebvunzo rwakaitwa nechiremba wezvirwere. Chiremba wenyu achatsanangura zvabuda mubvunzo yenyu zvakadzama uye zvazvinoreva pamamiriro enyu ezvinhu chaiwo.

Yeukai, kana mhinduro dzebvunzo dzisina kujairika , zvichireva kuti chiremba wezvirwere akawana zviratidzo zvechirwere, musavhunduka. Chiremba wenyu achakutsanangurirai mhinduro dzacho uye achakurukura nemi nzira dzenyu dzekurapa. Mune zvimwe zviitiko, timu yevanachiremba inogona kushanda pamwe chete kugadzira hurongwa hwakanakisa hwekurapa.

Chekupedzisira, zvinhu zvinonyanya kukosha zvekurangarira

Zvakanaka, kubva pane zvatakurukura, ndinovimba une pfungwa yakanaka nezvekudzivirira muviri kubva muzvirwere. Heano zvimwe zvinhu zvakakosha zvekurangarira:

• Kudzivirira mabhakitiriya (immunostaining) inzira yekuongorora ma antibodies mumasero edu inoshandisa ma antibodies kuti ione uye inyore ma antigen chaiwo ari mukati memasero edu.
• Kuongororwa uku kunogona kubatsira kuona zvirwere zvakasiyana-siyana, kusanganisira hutachiona hwemavhairasi nemabhakitiriya, pamwe nemamwe marudzi ekenza.
• Kana uchifanira kuongororwa kuti une immunostaining, hapana chikonzero chekutya.Chiremba wako achakutsanangurira zvakadzama.
• Kana zvabuda muongororo, taurai nachiremba wenyu pachena kuti zvinorevei padambudziko renyu uye kana muchida kurapwa kana kuti kwete.

Yeukai, sainzi yezvekurapa iri kuramba ichichinja. Kuburikidza nemiedzo yakaita seiyi ndiko kunoita kuti tikwanise kuona zvirwere zvakawanda nekukurumidza uye kuzvirapa nemazvo. Saka, kana chiremba akakurudzira bvunzo yakadaro, zvakakosha kugadzirira.

Kuchengetedza Dziviriro Mumuviri , Pathology, Antibodies, Antigens, Kuongororwa kweKenza, Kuongororwa kweLaboratory