Chiremba wako angangodaro akaraira bvunzo yakakosha yekusimbisa chirwere. Wakambonzwa here nezveshoko rekuti "immunostaining"? Ringanzwika serakaoma uye rakaoma. Asi chaizvoizvo ibvunzo yepamusoro-soro yerabhoritari inotibatsira kuongorora zvirwere zvakaita sekenza nezvirwere zvakasiyana-siyana. Nhasi, tichataura nezvazvo nenzira iri nyore.
Zvichitaurwa zviri nyore, chii chinonzi Immunostaining?
Kuchengetedza muviri (immunostaining) inzira yakakosha inoshandiswa kuona puroteni chaiyo iyo chiremba anoda kutsvaga pakati pemapuroteni akawanda ari muropa rako, sampuli yenyama, kana sampuli yemongo wemapfupa.
Fungidzira uri kuverenga bhuku. Unoshandisa peni yekusimbisa kuti uratidze mitsara inonyanya kukosha mubhuku kuitira kuti ugone kuiona zviri nyore. Ndizvo zvinoita nzira iyi. Kubva pazviuru zvemapuroteni ari mumasero emuviri wedu, nzira iyi inotibatsira kuziva mapuroteni chaiwo (atinoti maantigen ) ane chekuita nechirwere chakati ne "kuapenda."
"Kutsveyamisa" uku kunoitwa uchishandisa zvinhu zvakakosha zvinonzi ma antibodies . Kutaura chokwadi, antibody inogona kungobatana neantigen chaiyo inoshandiswa. Antibody iyi inosungirirwa kune pigment chaiyo, enzyme, fluorescent molecule, kana gold particles. Saka kana antibody iyoyo ikabatana neantigen iri kutaurwa nezvayo, patinoitarisa pasi pe microscope, inoshanduka kuita ruvara rwakanaka uye tinogona kuiona zvakajeka.
Chinhu chakakosha ndechekuti bvunzo iyi iitwe nenyanzvi dzezvirwere murabhoritari. Kunyangwe sampuli (yakadai seropa kana nyama) ikatorwa kubva kwauri kuchipatara, bvunzo yacho inoitirwa murabhoritari.
Ndedzipi mhando huru dzemiedzo yekudzivirira muviri?
Pane nzira dzakawanda dzinoshandiswa pakuita bvunzo iyi. Chiremba wako achasarudza nzira yakakodzera zvichienderana nemamiriro ako ehutano. Ngatitarisei dzimwe dzenzira huru.
| Nzira yekuedza | Izvi zvinobatsirei uye zvinoshandisirwei? |
|---|---|
| Kudzivirira hutachiona hwemuviri | Iyi ndiyo nzira inonyanya kushandiswa. Pano, tissue sampuro inotorwa kubva kwauri inoiswa pagirazi, antibody yakakodzera inowedzerwa, uye dhayi yakakosha inoshandiswa kuisvibisa. Inozoongororwa nemaikorosikopu. Izvi zvinobatsira zvikuru pakuona mhando dzakasiyana dzekenza , kupatsanura mamiriro etsinga, uye matambudziko eropa. |
| Kuyerera kwemasero eropa | Funga nezvazvo sekuskena zvekudya musupamaketi. Nenzira iyi, masero ako anopfuudzwa nemwaranzi yelaser, rimwe nerimwe, uchishandisa ma antibodies ane mavara. Muchina uyu unogona kuverenga, kurongedza, uye kupatsanura masero. Izvi zvinoshandiswa kuona kenza dzakadai seleukemia ne lymphoma, zvirwere zvakasiyana-siyana zvinotapukira, uye zvirwere zve autoimmune . |
| Kuongororwa kwe immuno-electron microscopy (EM immunolabeling) | Izvi zvinoita kuti sampuro ionekwe nekukwidziridzwa kwakanyanya, nenzira yakadzama. Pano, zvidimbu zvegoridhe zvecolloidal zvinonamirwa kuma antibodies. Kana zvikaonekwa uchishandisa maikorosikopu e electron, zvidimbu zvegoridhe izvi zvinoonekwa zvakajeka semadonhwe matema. Saka, kana antibody yabatana neantigen, inogona kuonekwa zviri nyore. Nzira iyi inoshandiswawo kuona hutachiona hwehutachiona uye kuwana nzvimbo dzemaantigen ehutachiona mumajekiseni. |
Ndinofanira kugadzirira sei bvunzo iyi?
Kazhinji, haufanirwe kugadzirira chero chinhu chakakosha pakuongororwa kwekudzivirira muviri.
Zvisinei, usati watora sampuro (kureva, kutora ropa kana chidimbu chenyama, senge biopsy), chiremba wako achakupa mirairo yakajeka yezvaunofanira kuita. Semuenzaniso, kana uri kuita biopsy, unogona kuudzwa kuti usadye kana kunwa chero chinhu usati waita oparesheni. Zvakakosha kutevedzera mirairo iyoyo nemazvo.
Bvunzo yacho inotora nguva yakareba sei? Mhinduro dzichawanikwa riini?
Nguva inotora kuti izvi zviitike inosiyana zvichienderana nenzira yakashandiswa.
- Dzimwe nzira dzinogona kuburitsa mhedzisiro mumaawa mashoma .
- Dzimwe nzira dzinogona kutora kusvika mazuva gumi .
Pazuva raunotorwa bvunzo yako, chiremba kana mushandi achakuudza kuti zvinotora nguva yakareba sei kuti uwane mhinduro, saka usazvidya mwoyo nazvo.
Mhedzisiro yacho inoti chii?
Chiremba wezvirwere ari murabhoritari achaongorora muenzaniso wako uye achatumira mushumo wakazara wezvakawanikwa kuna chiremba wako ari kukurapa. Chiremba wako achabatanidza zvawanikwa mumushumo nemamiriro ako ezvinhu uye achakutsanangurira zviri nyore kuti zvinorevei.
Chii chinoitika kana mhedzisiro yacho isina kujairika?
Kana bvunzo ikaratidza kuti une chirwere, usavhunduka. Chiremba wako achakutsanangurira zvabuda zvizere uye achakurukura nzira dzekurapa nadzo.
Dzimwe nguva, chikwata chevanachiremba vanobva kumatunhu akasiyana chinogona kuungana kuti vakurukure hurongwa hwekurapa hwakanakisa. Chinangwa chemunhu wese ndechekukupa mushonga wakanakisa. Saka, taura nachiremba wako nezvemibvunzo kana zvinokunetsa zvaungave nazvo.
Mharidzo Yekuenda Nayo Kumba
- Immunostaining iongororo yemurabhoritari inoshandisa ma antibodies kunyatsoongorora zvirwere zvakaita sekenza nehutachiona.
- Kune mhando dzakasiyana dzebvunzo iyi, uye chiremba wako achasarudza nzira yakakodzera zvichienderana nemamiriro ako ehutano.
- Chiremba wako achakuudza mirairo yekutevera usati wapa sampuro yekuongororwa.
- Nguva inotora kuti uwane mhinduro dzebvunzo inogona kusiyana, uye kana mhinduro dzangowanikwa, chiremba wako achakutsanangurira uye achakurukura matanho anotevera, kana zvichidikanwa.
- Kurukurai mibvunzo yenyu nezvemhinduro dzebvunzo dzenyu kana mamiriro ehutano pachena nachiremba wenyu.
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